A8IPV3 (ARGB_AZOC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	AZC_0701

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_B
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_B.
Sequence similarities	Belongs to the acetylglutamate kinase family.
Sequence caution	The sequence BAF86699.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	Acetylglutamate kinase HAMAP MF_00082_B		PRO_0000335608
Regions
	Region	72 – 73	2	Substrate binding By similarity
Sites
	Binding site	94	1	Substrate By similarity
	Binding site	199	1	Substrate By similarity
	Site	37	1	Transition state stabilizer By similarity
	Site	259	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8IPV3 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: 37D1487878CF7FEC	FASTA	301	31,667
	10 20 30 40 50 60 MSASIADNVA EAQLQAEVLT KALPHMQRYD DAIVVVKYGG HAMGNDELAR HFAQDIVLLE 70 80 90 100 110 120 QSGVNPVVVH GGGPQIGAML EKLGIKSEFA AGLRITDKAT IEIVEMVLAG SINKQIVGFI 130 140 150 160 170 180 NEAGGKAIGL CGKDGNMVRA RKATRTIVDP QSHIEEVVDL GFVGEPDTVD TMVLDQILGR 190 200 210 220 230 240 ELIPVLAPVA SAVDGGTYNV NADTFAGAIA GALGAKRLLL LTDVPGVLDK NKQLIPRLTI 250 260 270 280 290 300 DECRALIADG TVSGGMIPKV ETCIYALEKG VEGVVIMDGK LPHSVLLELL TDHGIGTLIT R

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43989 / DSM 5975 / ORS 571.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009384 Genomic DNA. Translation: BAF86699.1. Different initiation.
RefSeq	YP_001523617.1. NC_009937.1.
3D structure databases
ProteinModelPortal	A8IPV3.
ModBase	Search...
Protein-protein interaction databases
STRING	A8IPV3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5689789.
GenomeReviews	Gene locus AZC_0701 in contig AP009384_GR.
KEGG	azc:AZC_0701.
PATRIC	21017915. VBIAzoCau17976_0733.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG497643.
ProtClustDB	PRK00942.
Enzyme and pathway databases
BioCyc	ACAU438753:AZC_0701-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_AZOC5

Accession

Primary (citable) accession number: A8IPV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program