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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei630 – 6301ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciACAU438753:GJF3-570-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:AZC_0567
OrganismiAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)
Taxonomic identifieri438753 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeAzorhizobium
ProteomesiUP000000270 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 874874Leucine--tRNA ligasePRO_1000071108Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi438753.AZC_0567.

Structurei

3D structure databases

ProteinModelPortaliA8IMK0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi627 – 6315"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8IMK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDRYNARD AEPRWQAIWN ERGIFRTRND DPRPKFFVME MFPYPSGRIH
60 70 80 90 100
IGHGRNYVMG DVLARYKRMQ GFNVLHPMGW DSFGLPAENA AIERGIHPKS
110 120 130 140 150
WTYENIASMK EQLQLLGLSL DWNREVATCD PSYYAEQQRL FLDLFENDLA
160 170 180 190 200
YRKESEVNWD PVDNTVLANE QVIDGRGWRS GAVVERRKLN QWFFRITAFA
210 220 230 240 250
QELLDAIDTL DRWPDKVRLM QRNWIGRSEG LEVLFELSKG SVAAAGTSAV
260 270 280 290 300
KVYTTRPDTL FGASFLALSP DHPLTQHLAA TRPDLAAFVA DCKTGGTSAE
310 320 330 340 350
AIETQEKKGF DTGLTVVHPL DAARTVPVYV ANFVLMDYGT GAIFGCPAHD
360 370 380 390 400
QRDLDFARKY GLTVTPVVLP PGADPAVFAV GSEAYDGEGT LYNSQFLDGL
410 420 430 440 450
SIADAKEQVA RRLERFRVGE QPQGTRKVNF RLRDWGISRQ RYWGCPIPII
460 470 480 490 500
HCDSCGPVAV PREQLPVELP DDVTFDRPGN PLDRAKAWRD VPCPKCGAPA
510 520 530 540 550
RRETDTMDTF VDSSWYFLRY ASDNPDKPLD KDAVRHWLPV DQYIGGIEHA
560 570 580 590 600
ILHLLYSRFF TRALKACGRV DVAEPFAGLF TQGMIVHETY KDKDGRWLFP
610 620 630 640 650
EEVKLLGDGK AEKLDDGSPV TVGPPEKMSK SKKNVVPPEV VVDTYGVDAG
660 670 680 690 700
RWFMLSDTPP ERDSEWTQSG IEGAWRFVQR VWRQVQEAIE LGAPKGADKP
710 720 730 740 750
ASFDAAATAL RRAAHGLAHQ VADDVERLRF NVAVAHVHEF ANAFGQAIAS
760 770 780 790 800
ARTASPVPAD LAFALREAAE IVTRVVGPMV PHLAEECWAA LGYDTLLAEA
810 820 830 840 850
AWPKAEPELL VENTITLPIQ INGKKRDDIT VPRDATAAEV EAAVLEMESV
860 870
RRALDGKAPK RIIVVPQRIV NVVA
Length:874
Mass (Da):97,619
Last modified:December 4, 2007 - v1
Checksum:iB7DC946BFD5677C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009384 Genomic DNA. Translation: BAF86565.1.
RefSeqiWP_012169098.1. NC_009937.1.
YP_001523483.1. NC_009937.1.

Genome annotation databases

EnsemblBacteriaiBAF86565; BAF86565; AZC_0567.
KEGGiazc:AZC_0567.
PATRICi21017619. VBIAzoCau17976_0591.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009384 Genomic DNA. Translation: BAF86565.1.
RefSeqiWP_012169098.1. NC_009937.1.
YP_001523483.1. NC_009937.1.

3D structure databases

ProteinModelPortaliA8IMK0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi438753.AZC_0567.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF86565; BAF86565; AZC_0567.
KEGGiazc:AZC_0567.
PATRICi21017619. VBIAzoCau17976_0591.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciACAU438753:GJF3-570-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571."
    Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571.

Entry informationi

Entry nameiSYL_AZOC5
AccessioniPrimary (citable) accession number: A8IMK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 4, 2007
Last modified: May 27, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.