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A8IMK0 (SYL_AZOC5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:AZC_0567
OrganismAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]
Taxonomic identifier438753 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeAzorhizobium

Protein attributes

Sequence length874 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 874874Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000071108

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif627 – 6315"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6301ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8IMK0 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: B7DC946BFD5677C8

FASTA87497,619
        10         20         30         40         50         60 
MSTDRYNARD AEPRWQAIWN ERGIFRTRND DPRPKFFVME MFPYPSGRIH IGHGRNYVMG 

        70         80         90        100        110        120 
DVLARYKRMQ GFNVLHPMGW DSFGLPAENA AIERGIHPKS WTYENIASMK EQLQLLGLSL 

       130        140        150        160        170        180 
DWNREVATCD PSYYAEQQRL FLDLFENDLA YRKESEVNWD PVDNTVLANE QVIDGRGWRS 

       190        200        210        220        230        240 
GAVVERRKLN QWFFRITAFA QELLDAIDTL DRWPDKVRLM QRNWIGRSEG LEVLFELSKG 

       250        260        270        280        290        300 
SVAAAGTSAV KVYTTRPDTL FGASFLALSP DHPLTQHLAA TRPDLAAFVA DCKTGGTSAE 

       310        320        330        340        350        360 
AIETQEKKGF DTGLTVVHPL DAARTVPVYV ANFVLMDYGT GAIFGCPAHD QRDLDFARKY 

       370        380        390        400        410        420 
GLTVTPVVLP PGADPAVFAV GSEAYDGEGT LYNSQFLDGL SIADAKEQVA RRLERFRVGE 

       430        440        450        460        470        480 
QPQGTRKVNF RLRDWGISRQ RYWGCPIPII HCDSCGPVAV PREQLPVELP DDVTFDRPGN 

       490        500        510        520        530        540 
PLDRAKAWRD VPCPKCGAPA RRETDTMDTF VDSSWYFLRY ASDNPDKPLD KDAVRHWLPV 

       550        560        570        580        590        600 
DQYIGGIEHA ILHLLYSRFF TRALKACGRV DVAEPFAGLF TQGMIVHETY KDKDGRWLFP 

       610        620        630        640        650        660 
EEVKLLGDGK AEKLDDGSPV TVGPPEKMSK SKKNVVPPEV VVDTYGVDAG RWFMLSDTPP 

       670        680        690        700        710        720 
ERDSEWTQSG IEGAWRFVQR VWRQVQEAIE LGAPKGADKP ASFDAAATAL RRAAHGLAHQ 

       730        740        750        760        770        780 
VADDVERLRF NVAVAHVHEF ANAFGQAIAS ARTASPVPAD LAFALREAAE IVTRVVGPMV 

       790        800        810        820        830        840 
PHLAEECWAA LGYDTLLAEA AWPKAEPELL VENTITLPIQ INGKKRDDIT VPRDATAAEV 

       850        860        870 
EAAVLEMESV RRALDGKAPK RIIVVPQRIV NVVA 

« Hide

References

[1]"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571."
Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43989 / DSM 5975 / ORS 571.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009384 Genomic DNA. Translation: BAF86565.1.
RefSeqYP_001523483.1. NC_009937.1.

3D structure databases

ProteinModelPortalA8IMK0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING438753.AZC_0567.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF86565; BAF86565; AZC_0567.
GeneID5691868.
KEGGazc:AZC_0567.
PATRIC21017619. VBIAzoCau17976_0591.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAVVHCDAC.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycACAU438753:GJF3-570-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_AZOC5
AccessionPrimary (citable) accession number: A8IMK0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 4, 2007
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries