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Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

pckA

Organism
Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611SubstrateUniRule annotation
Binding sitei195 – 1951SubstrateUniRule annotation
Metal bindingi201 – 2011ManganeseUniRule annotation
Binding sitei201 – 2011ATPUniRule annotation
Binding sitei201 – 2011SubstrateUniRule annotation
Metal bindingi220 – 2201Manganese; via tele nitrogenUniRule annotation
Binding sitei220 – 2201ATPUniRule annotation
Metal bindingi257 – 2571ManganeseUniRule annotation
Binding sitei285 – 2851ATPUniRule annotation
Binding sitei323 – 3231ATPUniRule annotation
Binding sitei323 – 3231SubstrateUniRule annotation
Binding sitei448 – 4481ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi236 – 2449ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciACAU438753:GJF3-4110-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCKUniRule annotation
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckAUniRule annotation
Ordered Locus Names:AZC_4063
OrganismiAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)
Taxonomic identifieri438753 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeAzorhizobium
Proteomesi
  • UP000000270 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 537537Phosphoenolpyruvate carboxykinase [ATP]PRO_1000072373Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi438753.AZC_4063.

Structurei

3D structure databases

ProteinModelPortaliA8ILP8.
SMRiA8ILP8. Positions 11-533.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiPOG091H03CD.

Family and domain databases

CDDicd00484. PEPCK_ATP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP. 1 hit.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.

Sequencei

Sequence statusi: Complete.

A8ILP8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIETGLRNSS QGIETFGLRN LAGVHWNLTE PQLYEHAIAK GEARLAAGGA
60 70 80 90 100
LAANTGVHTG RSPKDKFVVR DATTEGEVWW DNNGSITPEQ FETLYQDFLA
110 120 130 140 150
AAEGKTLFAQ DLYGGADPAH RIAARVYTEY AWHSLFIRTM LRRPAREELP
160 170 180 190 200
GYVPELTIID LPSFKADPAR HGVRSDTIIA VNFTRRIVLI GSSSYAGEMK
210 220 230 240 250
KSVFTFLNYL LPAKGVMPMH CSANAGKDGD VALFFGLSGT GKTTLSADPA
260 270 280 290 300
RTLLGDDEHG WSNTGVFNFE GGCYAKTIRL SKEAEPEIFA ASDRFGTILE
310 320 330 340 350
NVILHEDTRV PDFDDGSLTE NTRSAYPLDF IPNASPTGRA GVPKNIIMLT
360 370 380 390 400
ADAFGVMPPI ARLTPAQAMY HFLSGYTAKV AGTEKGVKDP EATFSTCFGA
410 420 430 440 450
PFMPRHPSVY GNLLRDLIAK YQVDCWLVNT GWTGGKYGVG RRMPIKVTRT
460 470 480 490 500
LLTAALDGSL KDAAFRTDPY FGFSVPSSVP GIEPHILYPS KTWADKADFD
510 520 530
ATARKLVAMF RDNFAKFEGH VDAAVRDAQP QVRIAAE
Length:537
Mass (Da):58,634
Last modified:December 4, 2007 - v1
Checksum:i9E17CEC9EBAD0525
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009384 Genomic DNA. Translation: BAF90061.1.
RefSeqiWP_012172583.1. NC_009937.1.

Genome annotation databases

EnsemblBacteriaiBAF90061; BAF90061; AZC_4063.
KEGGiazc:AZC_4063.
PATRICi21025009. VBIAzoCau17976_4242.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009384 Genomic DNA. Translation: BAF90061.1.
RefSeqiWP_012172583.1. NC_009937.1.

3D structure databases

ProteinModelPortaliA8ILP8.
SMRiA8ILP8. Positions 11-533.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi438753.AZC_4063.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF90061; BAF90061; AZC_4063.
KEGGiazc:AZC_4063.
PATRICi21025009. VBIAzoCau17976_4242.

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiPOG091H03CD.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciACAU438753:GJF3-4110-MONOMER.

Family and domain databases

CDDicd00484. PEPCK_ATP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP. 1 hit.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPCKA_AZOC5
AccessioniPrimary (citable) accession number: A8ILP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 4, 2007
Last modified: September 7, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.