ID   MDH_AZOC5               Reviewed;         321 AA.
AC   A8ILC6;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 1.
DT   16-JUN-2009, entry version 12.
DE   RecName: Full=Malate dehydrogenase;
DE            EC=1.1.1.37;
GN   Name=mdh; OrderedLocusNames=AZC_4016;
OS   Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Xanthobacteraceae; Azorhizobium.
OX   NCBI_TaxID=438753;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T.,
RA   Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B.,
RA   Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M.,
RA   Oyaizu H.;
RT   "Complete genome sequence of the nitrogen-fixing bacterium
RT   Azorhizobium caulinodans ORS571.";
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the reversible oxidation of malate to
CC       oxaloacetate (By similarity).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = oxaloacetate + NADH.
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 3 family.
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DR   EMBL; AP009384; BAF90014.1; -; Genomic_DNA.
DR   RefSeq; YP_001526932.1; -.
DR   GeneID; 5687996; -.
DR   GenomeReviews; AP009384_GR; AZC_4016.
DR   KEGG; azc:AZC_4016; -.
DR   OMA; A8ILC6; MGWTTQE.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:HAMAP.
DR   GO; GO:0006096; P:glycolysis; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:HAMAP.
DR   HAMAP; MF_00487; -; 1.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR001236; Lactate/malate_DH.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR011275; Malate_DH_NAD-dep_bac.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   PRINTS; PR00086; LLDHDRGNASE.
DR   TIGRFAMs; TIGR01763; MalateDH_bact; 1.
PE   3: Inferred from homology;
KW   Complete proteome; NAD; Oxidoreductase; Tricarboxylic acid cycle.
FT   CHAIN         1    321       Malate dehydrogenase.
FT                                /FTId=PRO_1000072417.
FT   NP_BIND      10     15       NAD (By similarity).
FT   NP_BIND     119    121       NAD (By similarity).
FT   ACT_SITE    176    176       Proton acceptor (By similarity).
FT   BINDING      34     34       NAD (By similarity).
FT   BINDING      83     83       Substrate (By similarity).
FT   BINDING      89     89       Substrate (By similarity).
FT   BINDING      96     96       NAD (By similarity).
FT   BINDING     121    121       Substrate (By similarity).
FT   BINDING     152    152       Substrate (By similarity).
SQ   SEQUENCE   321 AA;  33677 MW;  2032149C7B46F9AE CRC64;
     MARSKIALIG AGQIGGTLAL LAGLKELGDV VLFDLVEGVP QGKALDLAEL SPVAGFDAAF
     AGTGAYEQIA GADVVIVTAG VPRKPGMSRD DLLAVNLKVM EQAGAGIAKY APDAFVICVT
     NPLDAMVWAL QKASGLPRHK VVGMAGVLDS ARLRYFLADE FNVSVEDVTA MVLGGHGDTM
     VPLMRYCTVA GIPVPDLIRI GWTSTDRIEA IVTRTRNGGA EIVELLKSGS AFYAPAASAI
     VMAESYLKDK KRVLPVAAPL SGEYGFRDLY VGVPAVIGAR GVERIVELEL DRAERAQFEQ
     SVASVQGLVD ACAKIAPDLV R
//