Reviewed, UniProtKB/Swiss-Prot A8ILC6 (MDH_AZOC5)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase
    EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	AZC_4016

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD(+) = oxaloacetate + NADH.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Malate dehydrogenase		PRO_1000072417
Regions
	Nucleotide binding	10 – 15	6	NAD By similarity
	Nucleotide binding	119 – 121	3	NAD By similarity
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	89	1	Substrate By similarity
	Binding site	96	1	NAD By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8ILC6-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 2032149C7B46F9AE	FASTA	321	33,677
	10 20 30 40 50 60 MARSKIALIG AGQIGGTLAL LAGLKELGDV VLFDLVEGVP QGKALDLAEL SPVAGFDAAF 70 80 90 100 110 120 AGTGAYEQIA GADVVIVTAG VPRKPGMSRD DLLAVNLKVM EQAGAGIAKY APDAFVICVT 130 140 150 160 170 180 NPLDAMVWAL QKASGLPRHK VVGMAGVLDS ARLRYFLADE FNVSVEDVTA MVLGGHGDTM 190 200 210 220 230 240 VPLMRYCTVA GIPVPDLIRI GWTSTDRIEA IVTRTRNGGA EIVELLKSGS AFYAPAASAI 250 260 270 280 290 300 VMAESYLKDK KRVLPVAAPL SGEYGFRDLY VGVPAVIGAR GVERIVELEL DRAERAQFEQ 310 320 SVASVQGLVD ACAKIAPDLV R

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP009384 Genomic DNA. Translation: BAF90014.1.
RefSeq	YP_001526932.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5687996.
GenomeReviews	Gene locus AZC_4016 in contig AP009384_GR.
KEGG	azc:AZC_4016.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00487. [Tree]
InterPro	IPR001557. L-lactate/malate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR011275. Malate_DHase_NAD-dep_bac. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_AZOC5

Accession

Primary (citable) accession number: A8ILC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 4, 2007
Last modified:	November 25, 2008
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)