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A8IJU8

- BIOB_AZOC5

UniProt

A8IJU8 - BIOB_AZOC5

Protein

Biotin synthase

Gene

bioB

Organism
Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi58 – 581Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi102 – 1021Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi133 – 1331Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi193 – 1931Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi266 – 2661Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciACAU438753:GJF3-364-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:AZC_0361
    OrganismiAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571)
    Taxonomic identifieri438753 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeAzorhizobium
    ProteomesiUP000000270: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 325325Biotin synthasePRO_0000381211Add
    BLAST

    Proteomic databases

    PRIDEiA8IJU8.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi438753.AZC_0361.

    Structurei

    3D structure databases

    ProteinModelPortaliA8IJU8.
    SMRiA8IJU8. Positions 18-320.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiFEIANEC.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A8IJU8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAAIAATAR PAAEMRTRAL YDLPLPELMF RAQAVHRAHF DACSVETAQL    50
    LSIKTGGCPE DCGYCSQSAH HDTGVAATRL MDVEAVLAEA RKAKAAGAAR 100
    FCMGAAWRAP KDRDMDAVCA MISGVRALGL ETCVTLGMLT PAQVDRLSAA 150
    GLDYYNHNVD TSPAYYPRIT STRTLDDRLE TLGHVRAGGI KVCCGGIVGM 200
    GETIEDRLAM LELLAGLDPH PESVPLNMWT PIDGTPVKAF EEKVDPIAFV 250
    RLVAVARLLM PRSVVRLSAG RHTLSDEAQA LCFLAGANSI FVGDTLLTTP 300
    NMEESRDAVL LARLGLRPAP AQPSA 325
    Length:325
    Mass (Da):34,656
    Last modified:December 4, 2007 - v1
    Checksum:i62ABD145AEB3B8C6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009384 Genomic DNA. Translation: BAF86359.1.
    RefSeqiWP_012168892.1. NC_009937.1.
    YP_001523277.1. NC_009937.1.

    Genome annotation databases

    EnsemblBacteriaiBAF86359; BAF86359; AZC_0361.
    GeneIDi5690093.
    KEGGiazc:AZC_0361.
    PATRICi21017191. VBIAzoCau17976_0377.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009384 Genomic DNA. Translation: BAF86359.1 .
    RefSeqi WP_012168892.1. NC_009937.1.
    YP_001523277.1. NC_009937.1.

    3D structure databases

    ProteinModelPortali A8IJU8.
    SMRi A8IJU8. Positions 18-320.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 438753.AZC_0361.

    Proteomic databases

    PRIDEi A8IJU8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAF86359 ; BAF86359 ; AZC_0361 .
    GeneIDi 5690093.
    KEGGi azc:AZC_0361.
    PATRICi 21017191. VBIAzoCau17976_0377.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi FEIANEC.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci ACAU438753:GJF3-364-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571."
      Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.
      Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43989 / DSM 5975 / ORS 571.

    Entry informationi

    Entry nameiBIOB_AZOC5
    AccessioniPrimary (citable) accession number: A8IJU8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3