A8IHK8 (KATG_AZOC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	AZC_3290

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	744 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 744	722	Catalase-peroxidase HAMAP MF_01961		PRO_0000354723
Sites
	Active site	115	1	Proton acceptor By similarity
	Metal binding	275	1	Iron (heme axial ligand) By similarity
	Site	111	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	114 ↔ 234		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-260) By similarity
	Cross-link	234 ↔ 260		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-114) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8IHK8 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 7CBBA65A5AE19CFB	FASTA	744	81,788
	10 20 30 40 50 60 MSPRARRCTD RCARMSERSM NATTETPTGK CPVAHGTGGT QNRDWWPNQL RVDLLNLHSP 70 80 90 100 110 120 KSDPLGAAFD YRAEFKKLDY EALKNDLRKL MTDSQDWWPA DFGNYGPQFV RMAWHSAGTY 130 140 150 160 170 180 RLADGRGGGG RGQQRFAPLN SWPDNVNIDK SRRLLWPIKQ KYGQKISWAD LMILTGNVAL 190 200 210 220 230 240 ETMGFRTFGF AGGREDTWEP DQDVFWGSET AWLSHRTLEK FDAPLGATEM GLIYVNPEGP 250 260 270 280 290 300 DRNGDPISAA KFIRETFARM AMNDEETVAL IGGGHTFGKT HGAAAESHKG PDPEAAALEA 310 320 330 340 350 360 QGLGWASNYG TGHGADTIGS GLEVTWTQTP AQWSNFFFEN LFKYEWVQTR SPAGAIQWEA 370 380 390 400 410 420 KDGPDIIPDA HNPEKKHKPT MLTTDLSLRF DPIYEKISRR FLENPQAFAE AFARAWFKLT 430 440 450 460 470 480 HRDLGPRSRY LGPEVPREVL LWQDPVPAVD HPLIDDADAA ALKAKVLASG LTVSELVGTA 490 500 510 520 530 540 WASASTFRGG DKRGGANGAR IRLAPQKDWA VNQPEQIDKV LKALTRIQGE FNLNASDGKK 550 560 570 580 590 600 VSLADVIVLA GNAGVEEAAK AAGHDVSVPF APGRTDASQA ETDADSFKWL EPAADGFRNY 610 620 630 640 650 660 QKDGLAVPAE VALIDKAQLL TLTAPELTVL IGGLRAININ VDGAKHGVFT DKPGALTTDF 670 680 690 700 710 720 FTNLLDMSTQ WKAAGESNDV YEGRDRQTGE LKWTGTRVDL VFGSNSILRA LAEVYAASDA 730 740 KDKFVTDFVA AWTKVMNLDR FDLA

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43989 / DSM 5975 / ORS 571.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009384 Genomic DNA. Translation: BAF89288.1.
RefSeq	YP_001526206.1. NC_009937.1.
3D structure databases
ProteinModelPortal	A8IHK8.
SMR	A8IHK8. Positions 39-744.
ModBase	Search...
Protein-protein interaction databases
STRING	A8IHK8.
Protein family/group databases
PeroxiBase	6665. AZcaCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5688109.
GenomeReviews	Gene locus AZC_3290 in contig AP009384_GR.
KEGG	azc:AZC_3290.
PATRIC	21023399. VBIAzoCau17976_3446.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	ACAU438753:AZC_3290-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_AZOC5

Accession

Primary (citable) accession number: A8IHK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program