Reviewed, UniProtKB/Swiss-Prot A8IFY6 (AROE_AZOC5)

Names and origin

Protein names

Recommended name:
    Shikimate dehydrogenase
    EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	AZC_0003

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Shikimate dehydrogenase HAMAP MF_00222		PRO_0000325101
Regions
	Nucleotide binding	127 – 131	5	NADP By similarity
Sites
	Active site	65	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	A8IFY6-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: B671580DF7A5C194	FASTA	281	29,633
	10 20 30 40 50 60 MKRVAIFGHP VIHSRSPLVH GYWLKQHGIA GDYVRHDVAP DEAEAFFARF AEQGYAGGNV 70 80 90 100 110 120 TIPHKEVAFN ALAEADPVAR ALGVANTLWL EDGRLFGANT DAPGFLSNLD VTAPGWDANP 130 140 150 160 170 180 RVALVLGAGG ASRAVLYGFL QRGFDKVLLA NRTLARAEAL AAHFGPRVVP IGWDGVSASL 190 200 210 220 230 240 KEAQVVANTT SLGMKGQPPL DLDLSVLADD AVVADVVYVP LETPLLKAAK ARGLRTVDGL 250 260 270 280 GMLLHQAVPG FARWFGVTPQ VDAGLRDLIV ADLAAKGQLG A

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP009384 Genomic DNA. Translation: BAF86001.1.
RefSeq	YP_001522919.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5690391.
GenomeReviews	Gene locus AZC_0003 in contig AP009384_GR.
KEGG	azc:AZC_0003.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8IFY6. YRAHEVT.
Family and domain databases
HAMAP	MF_00222. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Quinate/shikimate_5-DH. IPR013708. Shikimate_DH-bd_N. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. aroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_AZOC5

Accession

Primary (citable) accession number: A8IFY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	December 4, 2007
Last modified:	June 16, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)