A8IFX1 (SYD_AZOC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	AZC_3659

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000071083

Sequences

	Sequence		Length	Mass (Da)
	A8IFX1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: F45AEAB7B54AB16C	FASTA	590	66,324
	10 20 30 40 50 60 MHRYRTHTCG ALTEAQVGDT VRVSGWCHRI RDHGGVLFVD LRDHYGLTQV VFDPDSAAFA 70 80 90 100 110 120 AAEKVRAEWV IRVDGRVRLR PEGTENSELA TGKVEIYATD LEVLGPAAEL PLPVFGDQDY 130 140 150 160 170 180 PEDIRLRYRF LDLRRERIHG NIMKRGQIID SLRQRMKGQG FFEFQTPILT ASSPEGARDF 190 200 210 220 230 240 LVPSRIHPGK FYALPQAPQQ YKQLIMMSGF DRYFQIAPCF RDEDPRADRL PGEFYQLDLE 250 260 270 280 290 300 MSFVTQDDVF AAVEPVIRGV FEEFGGGKPV TQNWPRIPYA EALRKYGSDK PDLRNPLVMQ 310 320 330 340 350 360 NVSEHFRGSN FKVFARLLED PKNEVWAIPG PTGGSRAFCD RMNSWAQGEG QPGLGYIMWR 370 380 390 400 410 420 EGGEGAGPLA NNIGPERTAA IREQLGLKDG DAAFFVAGNP EKFYKFAGLA RTKVGEELKL 430 440 450 460 470 480 IDTERFELAW IVDFPFYEWS DEEKKIDFSH NPFSMPQGGL EALNGQDPLT IKAFQYDIAC 490 500 510 520 530 540 NGYEIASGGI RNHRPEAMVK AFELAGYDEA TVIERFGGMY RAFQYGAPPH GGMAAGIDRI 550 560 570 580 590 VMLLCGVSNL REISLFPMNQ QALDLLMGAP NEATPKQMKE LHIRPALPAK

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43989 / DSM 5975 / ORS 571.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009384 Genomic DNA. Translation: BAF89657.1.
RefSeq	YP_001526575.1. NC_009937.1.
3D structure databases
ProteinModelPortal	A8IFX1.
ModBase	Search...
Protein-protein interaction databases
STRING	A8IFX1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5687877.
GenomeReviews	Gene locus AZC_3659 in contig AP009384_GR.
KEGG	azc:AZC_3659.
PATRIC	21024167. VBIAzoCau17976_3826.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	YQLDVEM.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	ACAU438753:AZC_3659-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_AZOC5

Accession

Primary (citable) accession number: A8IFX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program