A8IES7 (ILVD_AZOC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	AZC_3530

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	617 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 617	617	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000070949
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A8IES7 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 3651B0272B565D2E	FASTA	617	65,732
	10 20 30 40 50 60 MPAYRSRTST HGRNMAGARG LWRATGMKDG DFGKPIIAVV NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AREIEKAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSREII ADSVEYMVNA HCADAMVCIS 130 140 150 160 170 180 NCDKITPGML MAAMRLNIPA VFVSGGPMEA GKVLLSTGEK KVDLIDAMIA AADDRVSDED 190 200 210 220 230 240 VKVMERSACP TCGSCSGMFT ANSMNCLTEA LGLALPGNGS VLATHADRER LFVEAGHLVV 250 260 270 280 290 300 DLARRYYEQD DAGVLPRTIA SFKAFENAMT LDISMGGSTN TVLHLLAAAN EGEVPFTMAD 310 320 330 340 350 360 IDRLSRKVPV LCKVAPAVAN IHMEDVHRAG GIMGILGELD RAGLIHTDLP TVHAPSMKDA 370 380 390 400 410 420 LERWDVTRTK AESVTTFYRA APGGVPTQVA FSQSRRWDDL DTDRASGVIR DFEHAYSKDG 430 440 450 460 470 480 GLAVLFGNIA EDGCIVKTAG VDASILKFTG PARIFESQDA AVEAILANGK IKAGDIVLIR 490 500 510 520 530 540 YEGPRGGPGM QEMLYPTSYL KSKGLGKACA LVTDGRFSGG SSGLSIGHVS PEAAEGGAIG 550 560 570 580 590 600 LVEEGDTIEI DIPNRRIHLA ISDEEMARRR AAMEAKGDAA WKPKDRQRVV SQALQAYAAL 610 TTSAARGAVR DVKSLRR

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43989 / DSM 5975 / ORS 571.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009384 Genomic DNA. Translation: BAF89528.1.
RefSeq	YP_001526446.1. NC_009937.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8IES7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5689396.
GenomeReviews	Gene locus AZC_3530 in contig AP009384_GR.
KEGG	azc:AZC_3530.
PATRIC	21023891. VBIAzoCau17976_3691.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	ACAU438753:AZC_3530-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_AZOC5

Accession

Primary (citable) accession number: A8IES7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program