ID   A8IE48_HUMAN            Unreviewed;       454 AA.
AC   A8IE48;
DT   04-DEC-2007, integrated into UniProtKB/TrEMBL.
DT   04-DEC-2007, sequence version 1.
DT   24-JAN-2024, entry version 126.
DE   SubName: Full=GA binding protein transcription factor alpha subunit 60kDa {ECO:0000313|EMBL:AFH41795.1};
DE   SubName: Full=GA binding protein transcription factor, alpha subunit 60kDa {ECO:0000313|EMBL:ACD11490.1, ECO:0000313|EMBL:EAX09967.1};
DE   SubName: Full=Nuclear respiratory factor 2 alpha subunit {ECO:0000313|EMBL:ABV90873.1};
GN   Name=GABPA {ECO:0000313|EMBL:ACD11490.1};
GN   ORFNames=hCG_401151 {ECO:0000313|EMBL:EAX09967.1};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606 {ECO:0000313|EMBL:ABV90873.1};
RN   [1] {ECO:0000313|EMBL:EAX09967.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=11181995; DOI=10.1126/science.1058040;
RA   Venter J.C., Adams M.D., Myers E.W., Li P.W., Mural R.J., Sutton G.G.,
RA   Smith H.O., Yandell M., Evans C.A., Holt R.A., Gocayne J.D., Amanatides P.,
RA   Ballew R.M., Huson D.H., Wortman J.R., Zhang Q., Kodira C.D., Zheng X.H.,
RA   Chen L., Skupski M., Subramanian G., Thomas P.D., Zhang J.,
RA   Gabor Miklos G.L., Nelson C., Broder S., Clark A.G., Nadeau J.,
RA   McKusick V.A., Zinder N., Levine A.J., Roberts R.J., Simon M., Slayman C.,
RA   Hunkapiller M., Bolanos R., Delcher A., Dew I., Fasulo D., Flanigan M.,
RA   Florea L., Halpern A., Hannenhalli S., Kravitz S., Levy S., Mobarry C.,
RA   Reinert K., Remington K., Abu-Threideh J., Beasley E., Biddick K.,
RA   Bonazzi V., Brandon R., Cargill M., Chandramouliswaran I., Charlab R.,
RA   Chaturvedi K., Deng Z., Di Francesco V., Dunn P., Eilbeck K.,
RA   Evangelista C., Gabrielian A.E., Gan W., Ge W., Gong F., Gu Z., Guan P.,
RA   Heiman T.J., Higgins M.E., Ji R.R., Ke Z., Ketchum K.A., Lai Z., Lei Y.,
RA   Li Z., Li J., Liang Y., Lin X., Lu F., Merkulov G.V., Milshina N.,
RA   Moore H.M., Naik A.K., Narayan V.A., Neelam B., Nusskern D., Rusch D.B.,
RA   Salzberg S., Shao W., Shue B., Sun J., Wang Z., Wang A., Wang X., Wang J.,
RA   Wei M., Wides R., Xiao C., Yan C., Yao A., Ye J., Zhan M., Zhang W.,
RA   Zhang H., Zhao Q., Zheng L., Zhong F., Zhong W., Zhu S., Zhao S.,
RA   Gilbert D., Baumhueter S., Spier G., Carter C., Cravchik A., Woodage T.,
RA   Ali F., An H., Awe A., Baldwin D., Baden H., Barnstead M., Barrow I.,
RA   Beeson K., Busam D., Carver A., Center A., Cheng M.L., Curry L.,
RA   Danaher S., Davenport L., Desilets R., Dietz S., Dodson K., Doup L.,
RA   Ferriera S., Garg N., Gluecksmann A., Hart B., Haynes J., Haynes C.,
RA   Heiner C., Hladun S., Hostin D., Houck J., Howland T., Ibegwam C.,
RA   Johnson J., Kalush F., Kline L., Koduru S., Love A., Mann F., May D.,
RA   McCawley S., McIntosh T., McMullen I., Moy M., Moy L., Murphy B.,
RA   Nelson K., Pfannkoch C., Pratts E., Puri V., Qureshi H., Reardon M.,
RA   Rodriguez R., Rogers Y.H., Romblad D., Ruhfel B., Scott R., Sitter C.,
RA   Smallwood M., Stewart E., Strong R., Suh E., Thomas R., Tint N.N., Tse S.,
RA   Vech C., Wang G., Wetter J., Williams S., Williams M., Windsor S.,
RA   Winn-Deen E., Wolfe K., Zaveri J., Zaveri K., Abril J.F., Guigo R.,
RA   Campbell M.J., Sjolander K.V., Karlak B., Kejariwal A., Mi H., Lazareva B.,
RA   Hatton T., Narechania A., Diemer K., Muruganujan A., Guo N., Sato S.,
RA   Bafna V., Istrail S., Lippert R., Schwartz R., Walenz B., Yooseph S.,
RA   Allen D., Basu A., Baxendale J., Blick L., Caminha M., Carnes-Stine J.,
RA   Caulk P., Chiang Y.H., Coyne M., Dahlke C., Mays A., Dombroski M.,
RA   Donnelly M., Ely D., Esparham S., Fosler C., Gire H., Glanowski S.,
RA   Glasser K., Glodek A., Gorokhov M., Graham K., Gropman B., Harris M.,
RA   Heil J., Henderson S., Hoover J., Jennings D., Jordan C., Jordan J.,
RA   Kasha J., Kagan L., Kraft C., Levitsky A., Lewis M., Liu X., Lopez J.,
RA   Ma D., Majoros W., McDaniel J., Murphy S., Newman M., Nguyen T., Nguyen N.,
RA   Nodell M., Pan S., Peck J., Peterson M., Rowe W., Sanders R., Scott J.,
RA   Simpson M., Smith T., Sprague A., Stockwell T., Turner R., Venter E.,
RA   Wang M., Wen M., Wu D., Wu M., Xia A., Zandieh A., Zhu X.;
RT   "The sequence of the human genome.";
RL   Science 291:1304-1351(2001).
RN   [2] {ECO:0000313|EMBL:EAX09967.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000313|EMBL:ABV90873.1}
RP   NUCLEOTIDE SEQUENCE.
RC   TISSUE=Hepatoma {ECO:0000313|EMBL:ABV90873.1};
RA   Yang S.J., Li K.N., Zhang Y.Q.;
RL   Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases.
RN   [4] {ECO:0000313|EMBL:ACD11490.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Livingston R.J., Rieder M.J., Shaffer T., Bertucci C., Baier C.N.,
RA   Rajkumar N., Willa H.T., Stanaway I.B., Nguyen C.P., Gildersleeve H.,
RA   Johnson E.J., Swanson J.E., McFarland I., Park C., Nickerson D.A.;
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN   [5] {ECO:0000313|EMBL:AFH41795.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Baron B.M.;
RT   "Isolation of a novel GABPalpha-interacting co-factor, E4TF-1 Binding
RT   Methyltransferase (EBM), which encodes a putative methyltransferase.";
RL   Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC       ECO:0000256|RuleBase:RU004019}.
CC   -!- SIMILARITY: Belongs to the ETS family. {ECO:0000256|ARBA:ARBA00005562,
CC       ECO:0000256|RuleBase:RU004019}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; EU159453; ABV90873.1; -; mRNA.
DR   EMBL; EU627790; ACD11490.1; -; Genomic_DNA.
DR   EMBL; JQ313900; AFH41795.1; -; mRNA.
DR   EMBL; CH471079; EAX09967.1; -; Genomic_DNA.
DR   RefSeq; NP_001184226.1; NM_001197297.1.
DR   RefSeq; NP_002031.2; NM_002040.3.
DR   RefSeq; XP_005260995.1; XM_005260938.4.
DR   RefSeq; XP_011527822.1; XM_011529520.2.
DR   RefSeq; XP_011527823.1; XM_011529521.2.
DR   RefSeq; XP_016883802.1; XM_017028313.1.
DR   SMR; A8IE48; -.
DR   Antibodypedia; 919; 448 antibodies from 32 providers.
DR   DNASU; 2551; -.
DR   GeneID; 2551; -.
DR   KEGG; hsa:2551; -.
DR   CTD; 2551; -.
DR   PharmGKB; PA28485; -.
DR   VEuPathDB; HostDB:ENSG00000154727; -.
DR   HOGENOM; CLU_037064_0_0_1; -.
DR   OMA; YSFWLQD; -.
DR   OrthoDB; 3915960at2759; -.
DR   BioGRID-ORCS; 2551; 546 hits in 1155 CRISPR screens.
DR   ChiTaRS; GABPA; human.
DR   GenomeRNAi; 2551; -.
DR   ExpressionAtlas; A8IE48; baseline and differential.
DR   GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR   GO; GO:0003682; F:chromatin binding; IEA:Ensembl.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IEA:Ensembl.
DR   GO; GO:0001825; P:blastocyst formation; IEA:Ensembl.
DR   GO; GO:0045653; P:negative regulation of megakaryocyte differentiation; IEA:Ensembl.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR   CDD; cd08534; SAM_PNT-GABP-alpha; 1.
DR   CDD; cd17039; Ubl_ubiquitin_like; 1.
DR   Gene3D; 1.10.150.50; Transcription Factor, Ets-1; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR000418; Ets_dom.
DR   InterPro; IPR046328; ETS_fam.
DR   InterPro; IPR024668; GABP_asu_N.
DR   InterPro; IPR003118; Pointed_dom.
DR   InterPro; IPR013761; SAM/pointed_sf.
DR   InterPro; IPR016312; TF_GA-bd_asu.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR11849; ETS; 1.
DR   PANTHER; PTHR11849:SF195; GA-BINDING PROTEIN ALPHA CHAIN; 1.
DR   Pfam; PF00178; Ets; 1.
DR   Pfam; PF11620; GABP-alpha; 1.
DR   Pfam; PF02198; SAM_PNT; 1.
DR   PIRSF; PIRSF001703; GABP_alpha; 1.
DR   PRINTS; PR00454; ETSDOMAIN.
DR   SMART; SM00413; ETS; 1.
DR   SMART; SM00251; SAM_PNT; 1.
DR   SUPFAM; SSF47769; SAM/Pointed domain; 1.
DR   SUPFAM; SSF54236; Ubiquitin-like; 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR   PROSITE; PS00345; ETS_DOMAIN_1; 1.
DR   PROSITE; PS00346; ETS_DOMAIN_2; 1.
DR   PROSITE; PS50061; ETS_DOMAIN_3; 1.
DR   PROSITE; PS51433; PNT; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004019};
KW   Nucleus {ECO:0000256|RuleBase:RU004019};
KW   Transcription {ECO:0000256|ARBA:ARBA00023163};
KW   Transcription regulation {ECO:0000256|ARBA:ARBA00023015}.
FT   DOMAIN          168..251
FT                   /note="PNT"
FT                   /evidence="ECO:0000259|PROSITE:PS51433"
FT   DOMAIN          320..400
FT                   /note="ETS"
FT                   /evidence="ECO:0000259|PROSITE:PS50061"
FT   REGION          297..316
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   454 AA;  51295 MW;  1AF2ABBBC79191DD CRC64;
     MTKREAEELI EIEIDGTEKA ECTEESIVEQ TYAPAECVSQ AIDINEPIGN LKKLLEPRLQ
     CSLDAHEICL QDIQLDPERS LFDQGVKTDG TVQLSVQVIS YQGIEPKLNI LEIVKPADTV
     EVVIDPDAHH AESEAHLVEE AQVITLDGTK HITTISDETS EQVTRWAAAL EGYRKEQERL
     GIPYDPIQWS TDQVLHWVVW VMKEFSMTDI DLTTLNISGR ELCSLNQEDF FQRVPRGEIL
     WSHLELLRKY VLASQEQQMN EIVTIDQPVQ IIPASVQSAT PTTIKVINSS AKAAKVQRAP
     RISGEDRSSP GNRTGNNGQI QLWQFLLELL TDKDARDCIS WVGDEGEFKL NQPELVAQKW
     GQRKNKPTMN YEKLSRALRY YYDGDMICKV QGKRFVYKFV CDLKTLIGYS AAELNRLVTE
     CEQKKLAKMQ LHGIAQPVTA VALATASLQT EKDN
//