A8ICW8 (SYA_AZOC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	AZC_2854

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	881 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 881	881	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000347496
Sites
	Metal binding	563	1	Zinc Potential
	Metal binding	567	1	Zinc Potential
	Metal binding	672	1	Zinc Potential
	Metal binding	676	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	A8ICW8 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: C967993014278427	FASTA	881	94,871
	10 20 30 40 50 60 MSGVNEIRSS FIDYFVKNGH EAVASSPLVP RNDPTLMFTN AGMVQFKNVF TGVEKRPYSR 70 80 90 100 110 120 AVTAQKCVRA GGKHNDLDNV GYTARHHTFF EMLGNFSFGD YFKDRAIELA WNLITKEWDL 130 140 150 160 170 180 PKDRLLATVY YDDDVAYDLW KKVAGLPDSR IIRIPTSDNF WAMGDTGPCG PCSEIFFDHG 190 200 210 220 230 240 DHIPGGPPGS PDEDGDRFIE IWNLVFMQYE QLPGERLNLP RPSIDTGMGL ERISALLQGT 250 260 270 280 290 300 HDNYETDLMR AIIAEVQELT NVPSNGPQKA SHRVIADHLR SSVFLVADGV LPSNEGRGYV 310 320 330 340 350 360 LRRIMRRAMR HAQLLGAREP LMHRLVPVLV REMGRAYPEI VRAEALATET LLLEETRFRR 370 380 390 400 410 420 TLERGLSLLE EESAGLVSGA RFPGEVAFKL YDTYGFPLDL TEDALRGRGI EVEREAFDAA 430 440 450 460 470 480 MERQKAEARA NWSGSGEAAT DTVWFGVRER EGATEFLGYD TETAEGAVRA LVQEGKEVSE 490 500 510 520 530 540 LPAGARGFVV LNQTPFYGES GGQVGDSGLV SGEGVKACVL NTQKKLGDVF VHDVEVTEGT 550 560 570 580 590 600 LKIGTPLALE VDHARRSAVR ANHSATHLLH EALRRVLGDH VAQKGSLVAP DRLRFDFSHP 610 620 630 640 650 660 KPLTADELAQ VEDIANTYVL RNEPVETRLM AVDDAVASGA RALFGEKYGD EVRVVSMGTD 670 680 690 700 710 720 SGNGAPFSVE LCGGTHVKRT GDIGIVTVLA DSGVAAGVRR LEALTATAAR RHLNAASTAL 730 740 750 760 770 780 QTTAGTLKVS TAEVEARVAA LVEERRKLER DLAEARKKLA MGGGGESGPA VIEVNGTRYL 790 800 810 820 830 840 RIAISGADPK DLKAIVDEGK TKIGSGVVAV ANTAADGKGA LVVGVTADLA GKFDAQKLVR 850 860 870 880 IGVEVLGGKG GGGRPDMAQG GGPDGAKADA ALAAIESALG A

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43989 / DSM 5975 / ORS 571.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009384 Genomic DNA. Translation: BAF88852.1.
RefSeq	YP_001525770.1. NC_009937.1.
3D structure databases
ProteinModelPortal	A8ICW8.
ModBase	Search...
Protein-protein interaction databases
STRING	A8ICW8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5688279.
GenomeReviews	Gene locus AZC_2854 in contig AP009384_GR.
KEGG	azc:AZC_2854.
PATRIC	21022479. VBIAzoCau17976_2989.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG354397.
OMA	MFTNSGM.
ProtClustDB	PRK00252.
Enzyme and pathway databases
BioCyc	ACAU438753:AZC_2854-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_AZOC5

Accession

Primary (citable) accession number: A8ICW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program