Reviewed, UniProtKB/Swiss-Prot A8IBS1 (DXS_AZOC5)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	AZC_3111

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	641 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity. HAMAP MF_00315
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 641	641	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000071993

Sequences

	Sequence		Length	Mass (Da)
	A8IBS1-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: CF49AAB6CF0AFA0E	FASTA	641	68,121
	10 20 30 40 50 60 MTVTKTPLLD TVRDASDVRR LPEEKLSQLA DELRAETISA VSVTGGHLGA GLGVVELTVA 70 80 90 100 110 120 LHHVFNTPHD RLIWDVGHQC YPHKILTGRR DRIRTLRQGG GLSGFTRRSE SEYDPFGAAH 130 140 150 160 170 180 SSTSISAGLG MAVARDLSGG DRNVIAVIGD GAMSAGMAYE AMNNAGAMDS RLIVILNDND 190 200 210 220 230 240 MSIAPPTGAM SAYLARLISG RTYLSLREIG KQLAGHLPKF VKDGAAKAEE FARGFWTGGT 250 260 270 280 290 300 LFEEMGFYYV GPIDGHNLEH LLPVLKNVRD AKTGPILVHV VTQKGKGYPP AEASADKYHG 310 320 330 340 350 360 VVKFDVVTGA QVKAKSNAPS YTRVFAETLI DEARRDDKVV AITAAMPSGT GLDLFGQAFP 370 380 390 400 410 420 QRTFDVGIAE QHAVTFAAGL AAEGYRPFCA LYSTFLQRGY DQVVHDVALQ GLPVRFAIDR 430 440 450 460 470 480 AGLVGADGAT HAGAFDLAML TCLPGFTVMA PADEAELTHM IATAVGHDAG PIAFRFPRGE 490 500 510 520 530 540 GVGVERPEKG ERLPIGKGRV VREGQDIAFL SLGTRLAACL DVAERLSSLG ISATVADARF 550 560 570 580 590 600 AKPIDRELIA HLARTHAALV VVEEGSVGGF GSQVLQFLSD EGLMDGGLKV RALTLPDTYI 610 620 630 640 DHDTPAAQLA EAGLDADGIY KRALALVQPA ERRGAVATKR A

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009384 Genomic DNA. Translation: BAF89109.1.
RefSeq	YP_001526027.1.
3D structure databases
SMR	A8IBS1. Positions 2-627.
ModBase	Search...
Genome annotation databases
GeneID	5690373.
GenomeReviews	Gene locus AZC_3111 in contig AP009384_GR.
KEGG	azc:AZC_3111.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571647.
OMA	QRFPDRY.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_AZOC5

Accession

Primary (citable) accession number: A8IBS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 4, 2007
Last modified:	February 9, 2010
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)