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Protein

Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase

Gene

phnN

Organism
Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of ribose 1,5-bisphosphate to 5-phospho-D-ribosyl alpha-1-diphosphate (PRPP).By similarity

Catalytic activityi

ATP + alpha-D-ribose 1,5-bisphosphate = ADP + 5-phospho-alpha-D-ribose 1-diphosphate.
Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate.

Pathwayi: 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route II).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase (phnN), Ribose 1,5-bisphosphate phosphokinase PhnN (phnN)
This subpathway is part of the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route II), the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis and in Metabolic intermediate biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 21ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Multifunctional enzyme, Transferase
Biological processAlkylphosphonate uptake
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00087; UER00175

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase
Including the following 2 domains:
Ribose 1,5-bisphosphate phosphokinase PhnN (EC:2.7.4.23)
Alternative name(s):
Ribose 1,5-bisphosphokinase
Putative thymidine phosphorylase (EC:2.4.2.4)
Alternative name(s):
TdRPase
Gene namesi
Name:phnN
Ordered Locus Names:AZC_2467
OrganismiAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)
Taxonomic identifieri438753 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeAzorhizobium
Proteomesi
  • UP000000270 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003146961 – 693Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylaseAdd BLAST693

Interactioni

Protein-protein interaction databases

STRINGi438753.AZC_2467

Structurei

3D structure databases

ProteinModelPortaliA8IA58
SMRiA8IA58
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 193Ribose 1,5-bisphosphokinaseAdd BLAST193
Regioni194 – 693ThymidinephosphorylaseAdd BLAST500

Sequence similaritiesi

In the N-terminal section; belongs to the ribose 1,5-bisphosphokinase family.Curated
In the C-terminal section; belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.Curated

Phylogenomic databases

eggNOGiENOG4108EB8 Bacteria
COG0213 LUCA
COG3709 LUCA
HOGENOMiHOG000252767
KOiK00758
OMAiQMVASIM
OrthoDBiPOG091H02BC

Family and domain databases

Gene3Di3.40.1030.102 hits
3.90.1170.301 hit
HAMAPiMF_00836 PhnN, 1 hit
MF_00703 Thymid_phosp_2, 1 hit
InterProiView protein in InterPro
IPR008145 GK/Ca_channel_bsu
IPR000312 Glycosyl_Trfase_fam3
IPR017459 Glycosyl_Trfase_fam3_N_dom
IPR036320 Glycosyl_Trfase_fam3_N_dom)sf
IPR035902 Nuc_phospho_transferase
IPR027417 P-loop_NTPase
IPR012699 PhnN
IPR036566 PYNP-like_C_sf
IPR013102 PYNP_C
IPR017872 Pyrmidine_PPase_CS
IPR028579 Thym_Pase_Put
IPR013466 Thymidine/AMP_Pase
IPR000053 Thymidine/pyrmidine_PPase
PANTHERiPTHR10515 PTHR10515, 1 hit
PfamiView protein in Pfam
PF02885 Glycos_trans_3N, 1 hit
PF00591 Glycos_transf_3, 1 hit
PF07831 PYNP_C, 1 hit
SMARTiView protein in SMART
SM00072 GuKc, 1 hit
SM00941 PYNP_C, 1 hit
SUPFAMiSSF47648 SSF47648, 1 hit
SSF52418 SSF52418, 1 hit
SSF52540 SSF52540, 1 hit
SSF54680 SSF54680, 1 hit
TIGRFAMsiTIGR02645 ARCH_P_rylase, 1 hit
TIGR02322 phosphon_PhnN, 1 hit
PROSITEiView protein in PROSITE
PS00647 THYMID_PHOSPHORYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

A8IA58-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVMGSGLFF FVVGPSGSGK DTLIEGAKAA LGPTGRYVFA RRAITRPADA
60 70 80 90 100
GGEAHEALSV DQFDAVLAQG GFLIHWEAHG LKYGLRATLL DDMAAGRHVI
110 120 130 140 150
ANGSRAMVAA LAERVPHLVV VEITAPEAVL AERLKGRGRE GAENIAARLE
160 170 180 190 200
RKVPPFPESV TVIQVPNDST PRAGIEKFVA ALVAQTARLR LVRMAIETGR
210 220 230 240 250
RNVAFLARGN TVVAAPDYLG PGRVDLIGEG RSIRAEVALV GDALLPSDAV
260 270 280 290 300
GLSSEAFGAL GLPEGAELVL TRTPVPESRA ALRRKIQGAT LDEGAYAQVV
310 320 330 340 350
GDIVEGRYPD SEVAGFLVAA DRSLSDDEVL ALAKVRAKFA SRITWDEPMV
360 370 380 390 400
VDKHSMGGIP GSRITMIVVP IVAAHGLAIP KTSSRAITSA AGTADAMETL
410 420 430 440 450
ARVDLDSAEV RRTVERARGC IAWNGRLSHS ALDDVMNAIT RPLGLDSTRW
460 470 480 490 500
SVASILSKKL AAGSTHVVID LPFGARARVK GAGEAHEMAR LFEQVGAGLG
510 520 530 540 550
LTVEAIPTDG SAPIGRGIGP ALEVRDVLWV LEDHSEAPPD LKEKALFFAS
560 570 580 590 600
RILAWDPAVG PDRARARAEE LLASGAARAA LERIVEAQGR WSEPVRPARL
610 620 630 640 650
THTVTAQNDG QVSDIDGFAV AGIARLAGAP LDKGAGIDLK ARVGDVVRAG
660 670 680 690
DPLFVIHAST AADLEAAAGA ALAFDGYGIM MDGKAFRRIN PER
Length:693
Mass (Da):72,760
Last modified:December 4, 2007 - v1
Checksum:i8847CD4C7646E9EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009384 Genomic DNA Translation: BAF88465.1

Genome annotation databases

EnsemblBacteriaiBAF88465; BAF88465; AZC_2467
KEGGiazc:AZC_2467

Similar proteinsi

Entry informationi

Entry nameiRBKTP_AZOC5
AccessioniPrimary (citable) accession number: A8IA58
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 4, 2007
Last modified: March 28, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome