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A8IA58 (RBKTP_AZOC5) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase

Including the following 2 domains:

  1. Ribose 1,5-bisphosphate phosphokinase PhnN
    EC=2.7.4.23
    Alternative name(s):
    Ribose 1,5-bisphosphokinase
  2. Putative thymidine phosphorylase
    EC=2.4.2.4
    Alternative name(s):
    TdRPase
Gene names
Name:phnN
Ordered Locus Names:AZC_2467
OrganismAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]
Taxonomic identifier438753 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeAzorhizobium

Protein attributes

Sequence length693 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of ribose 1,5-bisphosphate to 5-phospho-D-ribosyl alpha-1-diphosphate (PRPP) By similarity. HAMAP-Rule MF_00703

Catalytic activity

ATP + alpha-D-ribose 1,5-bisphosphate = ADP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_00703

Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate. HAMAP-Rule MF_00703

Pathway

Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route II): step 3/3. HAMAP-Rule MF_00703

Sequence similarities

In the N-terminal section; belongs to the ribose 1,5-bisphosphokinase family.

In the C-terminal section; belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 693693Bifunctional ribose 1,5-bisphosphokinase-thymidine phosphorylase HAMAP-Rule MF_00703
PRO_0000314696

Regions

Nucleotide binding14 – 218ATP By similarity
Region1 – 193193Ribose 1,5-bisphosphokinase HAMAP-Rule MF_00703
Region194 – 693500Thymidinephosphorylase HAMAP-Rule MF_00703

Sequences

Sequence LengthMass (Da)Tools
A8IA58 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: 8847CD4C7646E9EB

FASTA69372,760
        10         20         30         40         50         60 
MSVMGSGLFF FVVGPSGSGK DTLIEGAKAA LGPTGRYVFA RRAITRPADA GGEAHEALSV 

        70         80         90        100        110        120 
DQFDAVLAQG GFLIHWEAHG LKYGLRATLL DDMAAGRHVI ANGSRAMVAA LAERVPHLVV 

       130        140        150        160        170        180 
VEITAPEAVL AERLKGRGRE GAENIAARLE RKVPPFPESV TVIQVPNDST PRAGIEKFVA 

       190        200        210        220        230        240 
ALVAQTARLR LVRMAIETGR RNVAFLARGN TVVAAPDYLG PGRVDLIGEG RSIRAEVALV 

       250        260        270        280        290        300 
GDALLPSDAV GLSSEAFGAL GLPEGAELVL TRTPVPESRA ALRRKIQGAT LDEGAYAQVV 

       310        320        330        340        350        360 
GDIVEGRYPD SEVAGFLVAA DRSLSDDEVL ALAKVRAKFA SRITWDEPMV VDKHSMGGIP 

       370        380        390        400        410        420 
GSRITMIVVP IVAAHGLAIP KTSSRAITSA AGTADAMETL ARVDLDSAEV RRTVERARGC 

       430        440        450        460        470        480 
IAWNGRLSHS ALDDVMNAIT RPLGLDSTRW SVASILSKKL AAGSTHVVID LPFGARARVK 

       490        500        510        520        530        540 
GAGEAHEMAR LFEQVGAGLG LTVEAIPTDG SAPIGRGIGP ALEVRDVLWV LEDHSEAPPD 

       550        560        570        580        590        600 
LKEKALFFAS RILAWDPAVG PDRARARAEE LLASGAARAA LERIVEAQGR WSEPVRPARL 

       610        620        630        640        650        660 
THTVTAQNDG QVSDIDGFAV AGIARLAGAP LDKGAGIDLK ARVGDVVRAG DPLFVIHAST 

       670        680        690 
AADLEAAAGA ALAFDGYGIM MDGKAFRRIN PER 

« Hide

References

[1]"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571."
Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43989 / DSM 5975 / ORS 571.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009384 Genomic DNA. Translation: BAF88465.1.
RefSeqYP_001525383.1. NC_009937.1.

3D structure databases

ProteinModelPortalA8IA58.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING438753.AZC_2467.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF88465; BAF88465; AZC_2467.
GeneID5687914.
KEGGazc:AZC_2467.
PATRIC21021651. VBIAzoCau17976_2580.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0213.
HOGENOMHOG000252767.
KOK00758.
OMAMNAITRP.
OrthoDBEOG61ZTGG.
ProtClustDBCLSK956389.

Enzyme and pathway databases

BioCycACAU438753:GJF3-2497-MONOMER.
UniPathwayUPA00087; UER00175.

Family and domain databases

Gene3D3.40.1030.10. 1 hit.
3.40.50.300. 2 hits.
3.90.1170.30. 1 hit.
HAMAPMF_00703. Thymid_phosp_2.
MF_00836. PhnN.
InterProIPR008145. GK/Ca_channel_bsu.
IPR000312. Glycosyl_Trfase_fam3.
IPR017459. Glycosyl_Trfase_fam3_N_dom.
IPR027417. P-loop_NTPase.
IPR012699. PhnN.
IPR013102. PYNP_C.
IPR000053. Pyrmidine_PPase.
IPR017872. Pyrmidine_PPase_CS.
IPR028579. Thym_Pase_Put.
IPR013466. Thymidine/AMP_Pase.
[Graphical view]
PANTHERPTHR10515. PTHR10515. 1 hit.
PfamPF02885. Glycos_trans_3N. 1 hit.
PF00591. Glycos_transf_3. 1 hit.
PF07831. PYNP_C. 1 hit.
[Graphical view]
SMARTSM00072. GuKc. 1 hit.
SM00941. PYNP_C. 1 hit.
[Graphical view]
SUPFAMSSF47648. SSF47648. 1 hit.
SSF52418. SSF52418. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54680. SSF54680. 1 hit.
TIGRFAMsTIGR02645. ARCH_P_rylase. 1 hit.
TIGR02322. phosphon_PhnN. 1 hit.
PROSITEPS00647. THYMID_PHOSPHORYLASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRBKTP_AZOC5
AccessionPrimary (citable) accession number: A8IA58
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 4, 2007
Last modified: April 16, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways