A8I8B0 (PURL_AZOC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	AZC_2231

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 735	735	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000080546
Regions
	Nucleotide binding	106 – 117	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A8I8B0 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 683DD1ECFB04DE0B	FASTA	735	78,145
	10 20 30 40 50 60 MIPNTPSITP ELVAEHGLKP DEYQRFVELI GREPTITELG IVSAMWNEHC SYKSSKVWLR 70 80 90 100 110 120 TLPTKGPRVI QGPGENAGVV DIGDGLAVVF KMESHNHPSF IEPYQGAGTG VGGILRDVFT 130 140 150 160 170 180 MGARPIAALN ALRFGDPHHP RTRRLLAGVV AGIGGYGNSF GVPTVGGSVG FHERYNGNIL 190 200 210 220 230 240 VNAMAVGLAK TDEIFYAAAA GVGRSIVYLG SKTGRDGIHG ATMASAEFGA DAEEKRPTVQ 250 260 270 280 290 300 VGDPFAEKLL LEACLEIMQA GCVVAIQDMG AAGLTCSAVE MGAKGDLGVE LDLNAVPCRE 310 320 330 340 350 360 TGMTAYEMML SESQERMLMV IADGKEDQAE AIFRKWGLDF AIIGKTTDTL RFVVKHDGDV 370 380 390 400 410 420 KADLPIKELG DEAPEYYRPF TETPPRPVLG PSDVNHAVSV ADALERLIAS PDLCSKRWVW 430 440 450 460 470 480 EQYDHLILGN TVQRPGGDAA VVRVNEGPKA LALTTDVTPR YCEADPFEGG KQAVAEAWRN 490 500 510 520 530 540 LTAVGATPIA VTDNLNFGNP EKPEIMGQFV GCVKGIGAAC EALDFPVVSG NVSLYNETNG 550 560 570 580 590 600 QGILPTPTIG GVGLIDDVEQ SMTLAFKAAG EAIFVVGKTD GWLGSSAYLY TVCDREDGAP 610 620 630 640 650 660 PPVDLVAEKR NGDFVRGLIK DDFITAAHDV SDGGLLVAIA EMAMAGRIGA SVDGVPAGMP 670 680 690 700 710 720 AHSFWFGEDQ ARYVVTLPAN QAAEMVRRAE AAGVPVTKLG KTGGDKITLA NERPIFVEGL 730 RDRHDSWLPI YMGAN

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43989 / DSM 5975 / ORS 571.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009384 Genomic DNA. Translation: BAF88229.1.
RefSeq	YP_001525147.1. NC_009937.1.
3D structure databases
ProteinModelPortal	A8I8B0.
ModBase	Search...
Protein-protein interaction databases
STRING	A8I8B0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5689460.
GenomeReviews	Gene locus AZC_2231 in contig AP009384_GR.
KEGG	azc:AZC_2231.
PATRIC	21021157. VBIAzoCau17976_2335.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG311214.
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	ACAU438753:AZC_2231-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_AZOC5

Accession

Primary (citable) accession number: A8I8B0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program