A8I819 (SYE_AZOC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	AZC_2199

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium › Azorhizobium caulinodans

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00022
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Sequence caution	The sequence BAF88197.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Glutamate--tRNA ligase HAMAP-Rule MF_00022		PRO_0000330954
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP-Rule MF_00022
	Motif	239 – 243	5	"KMSKS" region HAMAP-Rule MF_00022
Sites
	Binding site	242	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8I819 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: C97C2CFD1F9AF53A	FASTA	471	52,534
	10 20 30 40 50 60 MSQIVTRFAP SPTGFLHIGG ARTALFNWLY AKAKGGKMLL RIEDTDRQRS TKEAIDAILE 70 80 90 100 110 120 GLTWLGIDWD GDVIYQFARA ERHRQAVEEM LSRGNAYPCY ATPEELDEMR ELARKEGRPP 130 140 150 160 170 180 RYDGRWRDRP ASERPTDRKP VIRLRAPQEG VTVIEDQVQG TVTFPNKDLD DLVLLRSDGT 190 200 210 220 230 240 PTYMLAVVVD DHDMGVTNII RGDDHLTNAA RQTQIYNALG WDVPRMAHIP LIHGPDGAKL 250 260 270 280 290 300 SKRHGALGVD AYRDMGYLPA ALRNYLVRLG WSHGDQEIFS TEEMVEHFDL DRVGRSAARF 310 320 330 340 350 360 DFQKLENING HYMRASSNAE LFDAVMGLIP HLPDAEHRLA RLTDARMEQL RAALPGLKER 370 380 390 400 410 420 AKTLTELLDG AEFIFATRPL LLEEKAQAVL TAEARAHIAA MIPQLEAADW SAAGTETVVR 430 440 450 460 470 AYAEQAGVKL GAVAQPLRAA LTGRTTSPPV FDVFAVLGRE ESLARLKDQT A

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43989 / DSM 5975 / ORS 571.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009384 Genomic DNA. Translation: BAF88197.1. Different initiation.
RefSeq	YP_001525115.1. NC_009937.1.
3D structure databases
ProteinModelPortal	A8I819.
ModBase	Search...
Protein-protein interaction databases
STRING	438753.AZC_2199.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAF88197; BAF88197; AZC_2199.
GeneID	5689283.
KEGG	azc:AZC_2199.
PATRIC	21021093. VBIAzoCau17976_2303.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000252722.
KO	K01885.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	ACAU438753:GJF3-2227-MONOMER.
Family and domain databases
Gene3D	1.10.10.350. 1 hit. 1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_00022_B. Glu_tRNA_synth_B.
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-ligase_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_AZOC5

Accession

Primary (citable) accession number: A8I819

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	May 1, 2013
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program