Reviewed, UniProtKB/Swiss-Prot A8I711 (DADA_AZOC5)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase small subunit
    EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	AZC_2066

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity.
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity.
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_1000073101
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	A8I711-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 1C3C7A1786C3BAAF	FASTA	417	45,246
	10 20 30 40 50 60 MRIIVLGAGV IGVTSAYFLA KAGHEVTVLD RQAGPALETS YANAGEVSPG YSSPWAAPGI 70 80 90 100 110 120 PMKAAKWLFM KHAPLIVRPT LDPVTWRWML QMLANCTSAR YAVNKGRMVR IAEYSRDVLM 130 140 150 160 170 180 QLRADTGIRY DERMQGTLEV FRSQKQLDGI AKDIAVLKAD GVPFEVLDRE GCVQVEPGLK 190 200 210 220 230 240 PAAHKIVGGL RLPGDETGDC FLFTNALAKL AEGLGVRFVY NVDLKRLRRD GDRIAAVETA 250 260 270 280 290 300 QGDYIADSYV AALGSYMPGF LAPLGLDLPI YPVKGYSITV PILDEAKAPV STVMDEYYKI 310 320 330 340 350 360 AITRLGSRIR VGGMAEIARF NKDLPPARQA TLTLSVEDLF GGAGDQKKAE FWCGLRPMTP 370 380 390 400 410 DGTPIIGKTK FGNLFLNGGH GTLGWTMSCG SARLLSDIIS GAKPEISTEG LDLSRYR

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP009384 Genomic DNA. Translation: BAF88064.1.
RefSeq	YP_001524982.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5688154.
GenomeReviews	Gene locus AZC_2066 in contig AP009384_GR.
KEGG	azc:AZC_2066.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8I711. MFQKHAP.
Family and domain databases
HAMAP	MF_01202. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

DADA_AZOC5

Accession

Primary (citable) accession number: A8I711

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 4, 2007
Last modified:	June 16, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)