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Protein

Tetraacyldisaccharide 4'-kinase

Gene

lpxK

Organism
Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).UniRule annotation

Catalytic activityi

ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate).UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Lipid-A-disaccharide synthase (AZC_1706)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi53 – 60ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00359; UER00482.

Names & Taxonomyi

Protein namesi
Recommended name:
Tetraacyldisaccharide 4'-kinaseUniRule annotation (EC:2.7.1.130UniRule annotation)
Alternative name(s):
Lipid A 4'-kinaseUniRule annotation
Gene namesi
Name:lpxKUniRule annotation
Ordered Locus Names:AZC_2383
OrganismiAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)
Taxonomic identifieri438753 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeAzorhizobium
Proteomesi
  • UP000000270 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000722741 – 338Tetraacyldisaccharide 4'-kinaseAdd BLAST338

Interactioni

Protein-protein interaction databases

STRINGi438753.AZC_2383.

Structurei

3D structure databases

SMRiA8I6A3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxK family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CHD. Bacteria.
COG1663. LUCA.
HOGENOMiHOG000004954.
KOiK00912.
OMAiMDDGFQN.
OrthoDBiPOG091H000L.

Family and domain databases

HAMAPiMF_00409. LpxK. 1 hit.
InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR003758. Tetraacyldisaccharide_4-kinase.
PANTHERiPTHR42724. PTHR42724. 1 hit.
PfamiView protein in Pfam
PF02606. LpxK. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00682. lpxK. 1 hit.

Sequencei

Sequence statusi: Complete.

A8I6A3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGQAPAFWS RRNSLMGWVL SPIGALVGAL TLKRMAGPST GVPVPVICIG
60 70 80 90 100
NPTVGGSGKT PTALMVLARL QEQGARPFAL LRGHGGRLAG PVLVDPQTHT
110 120 130 140 150
AADVGDEALL LAQATPTVVS RDRPAGAAHA VALGASHVVM DDGFQNPSLA
160 170 180 190 200
KDVSLLVIDG EAGVGNGRVL PAGPLRAPLA PQLDRADALL VAGGGRCADA
210 220 230 240 250
LGRLAHSHGK VVLRGQVRPD PSVIATLEAG TVLAFAGIGR PQKLADTLAA
260 270 280 290 300
AGVRIGRLQP FPDHHPYQPS DIAPLIAEAA RERWQLVTTT KDHVRLADRR
310 320 330
FADMAGAITA LPVTMQLDAP EALDDILRLA EARAAARS
Length:338
Mass (Da):34,919
Last modified:December 4, 2007 - v1
Checksum:iC69D2613DB86EAEA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009384 Genomic DNA. Translation: BAF88381.1.
RefSeqiWP_012170909.1. NC_009937.1.

Genome annotation databases

EnsemblBacteriaiBAF88381; BAF88381; AZC_2383.
KEGGiazc:AZC_2383.

Similar proteinsi

Entry informationi

Entry nameiLPXK_AZOC5
AccessioniPrimary (citable) accession number: A8I6A3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 4, 2007
Last modified: August 30, 2017
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families