A8I4X6 (SYC_AZOC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	AZC_1784

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Cysteine--tRNA ligase HAMAP MF_00041		PRO_1000071069
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	272 – 276	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	214	1	Zinc By similarity
	Metal binding	239	1	Zinc By similarity
	Metal binding	243	1	Zinc By similarity
	Binding site	275	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8I4X6 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 6EE7AED407ABF226	FASTA	462	52,099
	10 20 30 40 50 60 MELRLYNTLT RSKDAFRPLD PNLVRLYVCG PTVYDHAHIG NARPVIVFDV LFRLLRRLYG 70 80 90 100 110 120 ADHVRYVRNI TDVDDKINAR AAERSISIRE LTEETYAWFR DDTAALGCLR PDVEPRATEH 130 140 150 160 170 180 ILEMQALIER LVASGHAYVA EEHVLFHVPS MPEYGKLSRR PLDEMLNGAR VDVAPFKRDP 190 200 210 220 230 240 MDFVLWKPST EGQPGWPSPC GIATPGRPGW HIECSAMSWR HLGETFDIHG GGIDLVFPHH 250 260 270 280 290 300 ENEIAQSRCA FDSGSMAQVW MHNGFLLVEG EKMSKSLGNF VTIRELLTDW PGEVLRLAML 310 320 330 340 350 360 STHYRQPINW TRQGIGFAAK TLDKWYRVIG DVEAETGEGN PFESEIADRL GDDLNSPSAI 370 380 390 400 410 420 THLHHLADVA EHEDASSALK RRFKSAANLM GLLHETETEW RARQRAAVSV DPDAIAALIA 430 440 450 460 DRNAARKARD FATADHIREQ LASQGIVLMD NKDGTTSWEL TR

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43989 / DSM 5975 / ORS 571.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009384 Genomic DNA. Translation: BAF87782.1.
RefSeq	YP_001524700.1. NC_009937.1.
3D structure databases
ProteinModelPortal	A8I4X6.
ModBase	Search...
Protein-protein interaction databases
STRING	A8I4X6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5689325.
GenomeReviews	Gene locus AZC_1784 in contig AP009384_GR.
KEGG	azc:AZC_1784.
PATRIC	21020221. VBIAzoCau17976_1871.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG327651.
OMA	NVFDIHG.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	ACAU438753:AZC_1784-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_AZOC5

Accession

Primary (citable) accession number: A8I4X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program