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A8I4L8

- LIPA_AZOC5

UniProt

A8I4L8 - LIPA_AZOC5

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Protein

Lipoyl synthase

Gene

lipA

Organism
Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi57 – 571Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi83 – 831Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi87 – 871Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi90 – 901Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciACAU438753:GJF3-1769-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:AZC_1747
OrganismiAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571)
Taxonomic identifieri438753 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeAzorhizobium
ProteomesiUP000000270: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Lipoyl synthasePRO_1000071727Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi438753.AZC_1747.

Structurei

3D structure databases

ProteinModelPortaliA8I4L8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiTIRAVRH.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A8I4L8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVTVVNTLNR PRHPEKQNRP ETEVLRKPDW IRVKAPGSAG WSNTAGIVRA
60 70 80 90 100
NGLHTVCEEA GCPNIGECWE KKHATFMIMG DTCTRACSFC NVRTGMPKAL
110 120 130 140 150
DLDEPQKVGE AVAKLGLSHV VITSVDRDDL TDGGAEHFAR TIASIRKLSP
160 170 180 190 200
GTTIEILTPD FLRKPGAIEV VVAARPDVFN HNLETVPGKY LTVRPGARYF
210 220 230 240 250
HSLRLLQQVK ELDPSIFTKS GIMVGLGEER NEVLQLMDDL RAAEVDFMTI
260 270 280 290 300
GQYLQPTRKH HKVERFVTPD EFKAYETVAY AKGFLMVSSS PLTRSSHHAG
310
DDFAKLRAAR EAKLGRI
Length:317
Mass (Da):35,168
Last modified:December 4, 2007 - v1
Checksum:i0155BF76D29D96D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009384 Genomic DNA. Translation: BAF87745.1.
RefSeqiYP_001524663.1. NC_009937.1.

Genome annotation databases

EnsemblBacteriaiBAF87745; BAF87745; AZC_1747.
GeneIDi5688143.
KEGGiazc:AZC_1747.
PATRICi21020139. VBIAzoCau17976_1832.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009384 Genomic DNA. Translation: BAF87745.1 .
RefSeqi YP_001524663.1. NC_009937.1.

3D structure databases

ProteinModelPortali A8I4L8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 438753.AZC_1747.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAF87745 ; BAF87745 ; AZC_1747 .
GeneIDi 5688143.
KEGGi azc:AZC_1747.
PATRICi 21020139. VBIAzoCau17976_1832.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi TIRAVRH.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci ACAU438753:GJF3-1769-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571."
    Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43989 / DSM 5975 / ORS 571.

Entry informationi

Entry nameiLIPA_AZOC5
AccessioniPrimary (citable) accession number: A8I4L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 4, 2007
Last modified: November 26, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3