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A8I4L8

- LIPA_AZOC5

UniProt

A8I4L8 - LIPA_AZOC5

Protein

Lipoyl synthase

Gene

lipA

Organism
Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi57 – 571Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi62 – 621Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi83 – 831Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi87 – 871Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi90 – 901Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciACAU438753:GJF3-1769-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:AZC_1747
    OrganismiAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571)
    Taxonomic identifieri438753 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeAzorhizobium
    ProteomesiUP000000270: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 317317Lipoyl synthasePRO_1000071727Add
    BLAST

    Proteomic databases

    ProMEXiA8I4L8.

    Interactioni

    Protein-protein interaction databases

    STRINGi438753.AZC_1747.

    Structurei

    3D structure databases

    ProteinModelPortaliA8I4L8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiTIRAVRH.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A8I4L8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVTVVNTLNR PRHPEKQNRP ETEVLRKPDW IRVKAPGSAG WSNTAGIVRA    50
    NGLHTVCEEA GCPNIGECWE KKHATFMIMG DTCTRACSFC NVRTGMPKAL 100
    DLDEPQKVGE AVAKLGLSHV VITSVDRDDL TDGGAEHFAR TIASIRKLSP 150
    GTTIEILTPD FLRKPGAIEV VVAARPDVFN HNLETVPGKY LTVRPGARYF 200
    HSLRLLQQVK ELDPSIFTKS GIMVGLGEER NEVLQLMDDL RAAEVDFMTI 250
    GQYLQPTRKH HKVERFVTPD EFKAYETVAY AKGFLMVSSS PLTRSSHHAG 300
    DDFAKLRAAR EAKLGRI 317
    Length:317
    Mass (Da):35,168
    Last modified:December 4, 2007 - v1
    Checksum:i0155BF76D29D96D6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009384 Genomic DNA. Translation: BAF87745.1.
    RefSeqiYP_001524663.1. NC_009937.1.

    Genome annotation databases

    EnsemblBacteriaiBAF87745; BAF87745; AZC_1747.
    GeneIDi5688143.
    KEGGiazc:AZC_1747.
    PATRICi21020139. VBIAzoCau17976_1832.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009384 Genomic DNA. Translation: BAF87745.1 .
    RefSeqi YP_001524663.1. NC_009937.1.

    3D structure databases

    ProteinModelPortali A8I4L8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 438753.AZC_1747.

    Proteomic databases

    ProMEXi A8I4L8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAF87745 ; BAF87745 ; AZC_1747 .
    GeneIDi 5688143.
    KEGGi azc:AZC_1747.
    PATRICi 21020139. VBIAzoCau17976_1832.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi TIRAVRH.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci ACAU438753:GJF3-1769-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571."
      Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.
      Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43989 / DSM 5975 / ORS 571.

    Entry informationi

    Entry nameiLIPA_AZOC5
    AccessioniPrimary (citable) accession number: A8I4L8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3