A8I4J4 (SYS_AZOC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine--tRNA ligase
EC=6.1.1.11

Alternative name(s):
Seryl-tRNA synthetase
Short name=SerRS
Seryl-tRNA(Ser/Sec) synthetase

Gene names

Name:	serS
Ordered Locus Names:	AZC_1739

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity. HAMAP MF_00176
Catalytic activity	ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). HAMAP MF_00176 ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec). HAMAP MF_00176
Pathway	Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. HAMAP MF_00176
Subunit structure	Homodimer. The tRNA molecule binds across the dimer By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00176.
Domain	Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity. HAMAP MF_00176
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	seryl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Serine--tRNA ligase HAMAP MF_00176		PRO_1000071631
Regions
	Nucleotide binding	281 – 283	3	ATP By similarity
	Nucleotide binding	368 – 371	4	ATP By similarity
	Region	250 – 252	3	Serine binding By similarity
Sites
	Binding site	304	1	Serine By similarity
	Binding site	404	1	Serine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8I4J4 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: BB7E435A966625BF	FASTA	445	49,325
	10 20 30 40 50 60 MHDIKWIREE PEALVRALVR RQVAKDEAVA LRDKLLSLDT ARRETLTKLE GLLARRNAAS 70 80 90 100 110 120 KEIGQAKAAK DEARAAALMD EVAKLKVDVP ELEQSAKAIE GELDGLLAAI PNVPLESVPE 130 140 150 160 170 180 GKDEHDNVEL KVVGTKRNYA FTPKQHFELG EALGLMDFET ASKLSGARFV VNKGPLARLE 190 200 210 220 230 240 RALGQFFLDM HVEQHGYTEV NPPLLVRDET MYGTAQLPKF FDDQFAVYAG STQRAIGDET 250 260 270 280 290 300 DAERYWLIPT AEVPLTNLVR DSILSEDELP LRFTANTPCF RAEAGSAGRD TRGMIRQHQF 310 320 330 340 350 360 TKVEMVSITT PETSEEEHHR MLACAEAVLQ KLDLHYRVVT LCTGDMGFAS RKTFDIEVWL 370 380 390 400 410 420 PGQNAYREIS SCSVCGDFQA RRMNARYRPA EGKGPRFVHT LNGSGVAVGR ALVAVLETYQ 430 440 QEDGSIVVPD VLQSYMGGVT VIAKA

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43989 / DSM 5975 / ORS 571.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009384 Genomic DNA. Translation: BAF87737.1.
RefSeq	YP_001524655.1. NC_009937.1.
3D structure databases
ProteinModelPortal	A8I4J4.
SMR	A8I4J4. Positions 1-442.
ModBase	Search...
Protein-protein interaction databases
STRING	A8I4J4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5691171.
GenomeReviews	Gene locus AZC_1739 in contig AP009384_GR.
KEGG	azc:AZC_1739.
PATRIC	21020123. VBIAzoCau17976_1824.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG629391.
OMA	ERSIINY.
ProtClustDB	PRK05431.
Enzyme and pathway databases
BioCyc	ACAU438753:AZC_1739-MONOMER.
Family and domain databases
HAMAP	MF_00176. Ser_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR002317. Ser-tRNA-synth_IIa. IPR015866. Ser-tRNA-synth_IIa_N. IPR010978. tRNA-bd_arm. [Graphical view]
Gene3D	G3DSA:1.10.287.40. Ser-tRNA-synth_IIa_N. 1 hit.
KO	K01875.
PANTHER	PTHR11778. tRNA-synt_ser. 1 hit.
Pfam	PF02403. Seryl_tRNA_N. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTS	PR00981. TRNASYNTHSER.
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00414. SerS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYS_AZOC5

Accession

Primary (citable) accession number: A8I4J4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program