A8I438 (SYP_AZOC5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	AZC_1686

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01570
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01570
Subunit structure	Homodimer By similarity. HAMAP MF_01570
Subcellular location	Cytoplasm By similarity HAMAP MF_01570.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Proline--tRNA ligase HAMAP MF_01570		PRO_1000073594

Sequences

	Sequence		Length	Mass (Da)
	A8I438 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: A4D417A80A44552F	FASTA	440	49,023
	10 20 30 40 50 60 MRLSRYFLPI LREVPKEADI VSHRLMLRAG MIRQESAGIY AWLPLGLRVL NKICNIIREE 70 80 90 100 110 120 QNRTGAVELL MPTIQSADLW RESGRYDDYG KEMLRIKDRH ERDMLFGPTN EEMITEIVRG 130 140 150 160 170 180 SIKSYKSLPL NLYHIQWKFR DEVRPRFGVY RSREFLMKDA YSFDLDAEGA KHSYNKMFVA 190 200 210 220 230 240 YLRTFARMGL KAIPMVADTG PIGGNLSHEF IILASTGESE VFCHGDYLEM APPPADVNFD 250 260 270 280 290 300 DAAAIQQVVN DWTTLYAATE EKHDAATFAA IPAERQMAAR GIEVGHIFYF GTKYSAPFNA 310 320 330 340 350 360 KVLGPDGAEH LIHMGSYGIG PSRLVAAMIE ASHDDAGIIW PDAVAPFQVG ILNLKVGDSA 370 380 390 400 410 420 VDAACADLYA KLTAAGVDVL YDDTDERAGS KFATADLIGL PWQILVGPKS LADGKVELKR 430 440 RVDGSRELVT PAEAFERLKG

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43989 / DSM 5975 / ORS 571.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009384 Genomic DNA. Translation: BAF87684.1.
RefSeq	YP_001524602.1. NC_009937.1.
3D structure databases
ProteinModelPortal	A8I438.
SMR	A8I438. Positions 1-438.
ModBase	Search...
Protein-protein interaction databases
STRING	A8I438.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5688875.
GenomeReviews	Gene locus AZC_1686 in contig AP009384_GR.
KEGG	azc:AZC_1686.
PATRIC	21020011. VBIAzoCau17976_1768.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG403504.
OMA	IQPAELW.
ProtClustDB	PRK12325.
Enzyme and pathway databases
BioCyc	ACAU438753:AZC_1686-MONOMER.
Family and domain databases
HAMAP	MF_01570. Pro_tRNA_synth_type2. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023716. Prolyl-tRNA_Synthase_IIa_type2. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_AZOC5

Accession

Primary (citable) accession number: A8I438

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 4, 2007
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program