Reviewed, UniProtKB/Swiss-Prot A8I1V7 (PURA_AZOC5)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AdSS
    AMPSase

Gene names

Name:	purA
Ordered Locus Names:	AZC_4670

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis By similarity.
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Adenylosuccinate synthetase HAMAP MF_00011		PRO_0000336999
Regions
	Nucleotide binding	12 – 18	7	GTP Potential
Sites
	Active site	141	1	By similarity
	Active site	148	1	By similarity
	Metal binding	13	1	Magnesium By similarity
	Metal binding	40	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8I1V7-1 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: 189F74955F4F19FC	FASTA	430	46,513
	10 20 30 40 50 60 MANVVVVGSQ WGDEGKGKIV DWLSEQADVV VRFQGGHNAG HTLVVDGVTY KLSLLPSGVV 70 80 90 100 110 120 RPGKLSVIGN GVVLDPQALV DELARLESQG VHVGPDRLRI AENTPLILPL HRELDALREN 130 140 150 160 170 180 ASEGTRIGTT KRGIGPAYED KVGRRAIRLV DLVDPKALPA KVERLLTHHN LIRRGLGVEE 190 200 210 220 230 240 VNPQALVDEL LALAPKVLPY MDRVWELLDK ARKDGKKILF EGAQGALLDI DHGTYPYVTS 250 260 270 280 290 300 SNTVASSAAG GSGVGPGALD YVLGITKAYT TRVGEGPFPT ELTDEVGKTL GTKGREFGVV 310 320 330 340 350 360 TGRPRRCGWF DAVLVRQTVR TCGIHGIALT KLDVLDGFDE IKVCVGYKLD GKEIDYFPSE 370 380 390 400 410 420 MGAQARVEPI YETIEGWTDS TAGARSWADL PAEAVKYVRW LEELIGCPVA VLSTSPERND 430 TILVHNPFQA

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP009384 Genomic DNA. Translation: BAF90668.1. Different initiation.
RefSeq	YP_001527586.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5687884.
GenomeReviews	Gene locus AZC_4670 in contig AP009384_GR.
KEGG	azc:AZC_4670.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00011. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_AZOC5

Accession

Primary (citable) accession number: A8I1V7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	June 16, 2009
This is version 14 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)