ID   A8I0V5_AZOC5            Unreviewed;      1835 AA.
AC   A8I0V5;
DT   04-DEC-2007, integrated into UniProtKB/TrEMBL.
DT   04-DEC-2007, sequence version 1.
DT   27-MAR-2024, entry version 85.
DE   RecName: Full=cellulase {ECO:0000256|ARBA:ARBA00012601};
DE            EC=3.2.1.4 {ECO:0000256|ARBA:ARBA00012601};
GN   OrderedLocusNames=AZC_1301 {ECO:0000313|EMBL:BAF87299.1};
OS   Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / LMG
OS   6465 / NBRC 14845 / NCIMB 13405 / ORS 571).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC   Xanthobacteraceae; Azorhizobium.
OX   NCBI_TaxID=438753 {ECO:0000313|EMBL:BAF87299.1, ECO:0000313|Proteomes:UP000000270};
RN   [1] {ECO:0000313|EMBL:BAF87299.1, ECO:0000313|Proteomes:UP000000270}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 /
RC   NCIMB 13405 / ORS 571 {ECO:0000313|Proteomes:UP000000270};
RX   PubMed=17720818; DOI=10.1128/AEM.01514-07;
RA   Suzuki S., Aono T., Lee KB., Suzuki T., Liu CT., Miwa H., Wakao S., Iki T.,
RA   Oyaizu H.;
RT   "Rhizobial factors required for stem nodule maturation and maintenance in
RT   Sesbania rostrata-Azorhizobium caulinodans ORS571 symbiosis.";
RL   Appl. Environ. Microbiol. 73:6650-6659(2007).
RN   [2] {ECO:0000313|Proteomes:UP000000270}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 /
RC   NCIMB 13405 / ORS 571 {ECO:0000313|Proteomes:UP000000270};
RA   Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T.,
RA   Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B.,
RA   Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.;
RT   "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium
RT   caulinodans ORS571.";
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000313|EMBL:BAF87299.1, ECO:0000313|Proteomes:UP000000270}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 /
RC   NCIMB 13405 / ORS 571 {ECO:0000313|Proteomes:UP000000270};
RX   PubMed=18522759; DOI=10.1186/1471-2164-9-271;
RA   Lee KB., Backer P.D., Aono T., Liu CT., Suzuki S., Suzuki T., Kaneko T.,
RA   Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B.,
RA   Binnewies T.T., Ussery D.W., D'Haeze W., Herder J.D., Gevers D.,
RA   Vereecke D., Holsters M., Oyaizu H.;
RT   "The genome of the versatile nitrogen fixer Azorhizobium caulinodans
RT   ORS571.";
RL   BMC Genomics 9:271-271(2008).
RN   [4] {ECO:0000313|EMBL:BAF87299.1, ECO:0000313|Proteomes:UP000000270}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 /
RC   NCIMB 13405 / ORS 571 {ECO:0000313|Proteomes:UP000000270};
RX   PubMed=19542345; DOI=10.1128/AEM.00398-09;
RA   Tsukada S., Aono T., Akiba N., Lee KB., Liu CT., Toyazaki H., Oyaizu H.;
RT   "Comparative genome-wide transcriptional profiling of Azorhizobium
RT   caulinodans ORS571 grown under free-living and symbiotic conditions.";
RL   Appl. Environ. Microbiol. 75:5037-5046(2009).
RN   [5] {ECO:0000313|EMBL:BAF87299.1, ECO:0000313|Proteomes:UP000000270}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 /
RC   NCIMB 13405 / ORS 571 {ECO:0000313|Proteomes:UP000000270};
RX   PubMed=20382809; DOI=10.1128/AEM.00238-10;
RA   Akiba N., Aono T., Toyazaki H., Sato S., Oyaizu H.;
RT   "phrR-like gene praR of Azorhizobium caulinodans ORS571 is essential for
RT   symbiosis with Sesbania rostrata and is involved in expression of reb
RT   genes.";
RL   Appl. Environ. Microbiol. 76:3475-3485(2010).
RN   [6] {ECO:0000313|EMBL:BAF87299.1, ECO:0000313|Proteomes:UP000000270}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 /
RC   NCIMB 13405 / ORS 571 {ECO:0000313|Proteomes:UP000000270};
RX   PubMed=21571889; DOI=10.1128/AEM.02327-10;
RA   Liu CT., Lee KB., Wang YS., Peng MH., Lee KT., Suzuki S., Suzuki T.,
RA   Oyaizu H.;
RT   "Involvement of the azorhizobial chromosome partition gene (parA) in the
RT   onset of bacteroid differentiation during Sesbania rostrata stem nodule
RT   development.";
RL   Appl. Environ. Microbiol. 77:4371-4382(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00000966};
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DR   EMBL; AP009384; BAF87299.1; -; Genomic_DNA.
DR   RefSeq; WP_012169829.1; NC_009937.1.
DR   SMR; A8I0V5; -.
DR   STRING; 438753.AZC_1301; -.
DR   CAZy; CBM2; Carbohydrate-Binding Module Family 2.
DR   CAZy; GH5; Glycoside Hydrolase Family 5.
DR   KEGG; azc:AZC_1301; -.
DR   eggNOG; COG2730; Bacteria.
DR   HOGENOM; CLU_237268_0_0_5; -.
DR   Proteomes; UP000000270; Chromosome.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030247; F:polysaccharide binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0007154; P:cell communication; IEA:InterPro.
DR   Gene3D; 2.60.40.2030; -; 8.
DR   Gene3D; 2.60.40.290; -; 2.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR038081; CalX-like_sf.
DR   InterPro; IPR003644; Calx_beta.
DR   InterPro; IPR001919; CBD2.
DR   InterPro; IPR008965; CBM2/CBM3_carb-bd_dom_sf.
DR   InterPro; IPR012291; CBM2_carb-bd_dom_sf.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR018087; Glyco_hydro_5_CS.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR11878:SF65; NA_CA-EXCHANGE PROTEIN, ISOFORM G; 1.
DR   PANTHER; PTHR11878; SODIUM/CALCIUM EXCHANGER; 1.
DR   Pfam; PF03160; Calx-beta; 8.
DR   Pfam; PF00553; CBM_2; 2.
DR   Pfam; PF00150; Cellulase; 1.
DR   SMART; SM00237; Calx_beta; 8.
DR   SMART; SM00637; CBD_II; 2.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF141072; CalX-like; 8.
DR   SUPFAM; SSF49384; Carbohydrate-binding domain; 2.
DR   PROSITE; PS51173; CBM2; 2.
DR   PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
PE   4: Predicted;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00023295};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000270};
KW   Repeat {ECO:0000256|ARBA:ARBA00022737};
KW   Signal {ECO:0000256|ARBA:ARBA00022729}.
FT   DOMAIN          780..888
FT                   /note="CBM2"
FT                   /evidence="ECO:0000259|PROSITE:PS51173"
FT   DOMAIN          1730..1835
FT                   /note="CBM2"
FT                   /evidence="ECO:0000259|PROSITE:PS51173"
FT   REGION          1719..1740
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1835 AA;  187867 MW;  3F0B876797249D8D CRC64;
     MADTSTSSGR IFVVDTAGAD IIGFDPAKDK LNLGDNSVHN FIVVDTPTGV GFMNPWSGET
     LIVQGVSLAQ LTIDSFVPIV NDHLRQTLSG ALAWEHGIVP APHTVYARSH ELGQIDKVAF
     DPATDVVDFR YFGTREQIYM SDSSEGVIIS NSGTGQALIL LGVTKAQLTV NNFVFYSAEV
     REDRVHLQLG FTTVPDSQVI PQGVEIAGTN TWPTAAGSGE APSGVTGTTY KIEWHYDSST
     TLAFNPAVDK LDFGWFKANE FDISEVNGST VISITNNHQT YTLTGVKIGA LAINNIIALD
     TSARAEWQST IDAAPKPVAP PVINLSDAQV VEGDAGAAQM LFTVSLSHAA DGPVSVSYST
     LNGTATAGTD YTSAVGTVTF AAGETQKTIA IAVTGDTLHE LNEAFSLQLS APSGATINDG
     SAVGTIIDND PDPSPGVPPK ISIADISVVE GNTDMAHFRF QVTLDKASTE TITVHYASSD
     LTATGGTDYE ILDGTLTFAP GETTQTIHAH MYSDTVVEPD ETYKVNLSNP TNATIADGTA
     IATIVNDDLA APLPTLALAD AAISEGNSGS KLLSFTVTLS AASATAVSVS YATGDGTATG
     GSDYTAGTGT VTFAPGETSK TIQVAVKGDT VVEANETFTV TLSAPSGATI ADGSAIGTIT
     NDDVAVVVPT VSIADASVVE GNSGTTALSF VVSLSAASTS AVSVSYATAN GTATAGSDYT
     AKTGTVTFAP GETSKTIQVA VTGDTAVEAN ETFTVALSSP SGATLGHGTA TATITNDDVS
     STPGGGAVVD YSVTSNWGAG FTAAMTVEAG AGALAGWTVE FDAGFTITNI WNATIVSHVG
     THYVLKNMSY NASVGAGKDT SFGFQATAGS GGTAASGFLI NGQPSGGGLP PTLPTLSVSD
     ASVLEGNSGT SDLAFTVALS AASATAVTVA YGTANGTATA GSDFTAASGT LTFAPGETSK
     VVHVAVTGDR TVEANETLAL KLSAPTGATL VRATATGTIT NDDAAVVVPT VSVSDASVTE
     GNSGTKDLVF NVTLSQAATG PVTLRYATAD GTATAGSDYG ATSGTLTFAA GETSKQVHVQ
     VTGDTLVEGN ETLSLTLSSP TGATIADGSG AGLITNDDVA PVVPTVSVAD ASVNEGASGI
     TDLAFTVSLS KATTAPVSLH YATADGTATA GSDYVAGSGT ITFAAGETSK VVHVQVNGDT
     AVEANETLKL ILSQPAGATI ATGTATGTIV NDDVTPGGGT PAVSIGDVTV TEGNPAVATA
     TGWFSTSGNQ IIDADGNSVQ IAGVNWFGFE SNTGSPHGLW TRGYKEMMQQ MLDEGFNTIR
     LPFSSDMLHS TGQAGSIDYS KNPDLKGLTS LQVMDKIVQY ADEIGIKIIL DHHRSSAGDG
     TSANGLWYDS SHSEASWIAD WQMLATRYAG DTSVIGADLH NEPYNGTWGD GGTNDWARAA
     TAAGNAIGAV NDNWLIFVEG IGTYQGQNYW WGGNLMGVRD HPIELNVDNK LVYSAHDYPN
     SVYAQPWFQG ANFAADLPAK FDQMWGFIYK EQIAPVYIGE FGTKLTDPKD VAWFKAITAY
     LGGDFNNDGV KDISAGEHGP SWTFWSWNPN SSDTGGILQN DWSTVNENKM VYLTPIEAPF
     PALDGSGGGV PGVHADFQVT LSQAASTAVS VEYHTVAPDA ASAADFTASS GTVVFQPGET
     TKVISIAITA DKAAEATEHF AVVLSNPVGV SVAKGTGTGT ILDNDGTPSS PTTPTTPTHT
     DLHGTLTVVD AWNSGFNANI SLHNDGTIPT SGWQVKVEMP NEISSIWNAQ ILSHDADGYV
     IGNAAWNGTV GADAEISFGF TATGHLDPTH VGLLF
//