Reviewed, UniProtKB/Swiss-Prot A8HZX8 (PYRD_AZOC5)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	AZC_4640

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O(2) = orotate + H(2)O(2).
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6.
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane proteinBy similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Dihydroorotate dehydrogenase		PRO_1000071765
Sites
	Active site	174	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8HZX8-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 291AF104984E9FA6	FASTA	356	37,501
	10 20 30 40 50 60 MDLYPLVRPF LPLFTPEQAH GLSIRALKKG LVPADRSPAD PVLRTRVWNI DFPNPVGLAA 70 80 90 100 110 120 GFDKDAEIID PLLSLGFGFV EAGSVTPRPQ PGNPKPRLFR LDEDEGVINR FGFNSQGLAP 130 140 150 160 170 180 FIYQLGKRKA AGLPGIVGAN VGKNKETEDA SEDYVAGVSA TCRLADYIVC NVSSPNTPGL 190 200 210 220 230 240 RLLQARTEMS ALIGAALSAR NDSLPDAATR PPLLVKVAPD LDDAGLEAVA EVTLELGVDG 250 260 270 280 290 300 IIMGNTTISR PDSLRSRHKG ETGGLSGAPL FTLSTERLGA LYRLVRGRIP LVGAGGIASG 310 320 330 340 350 ADAYAKIRAG ASLVQLYSAL VFHGPALVPR IKADLAARLK ADGFRSVADA VGADIR

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP009384 Genomic DNA. Translation: BAF90638.1.
RefSeq	YP_001527556.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5687762.
GenomeReviews	Gene locus AZC_4640 in contig AP009384_GR.
KEGG	azc:AZC_4640.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. DHO_DHase_1_2. IPR005719. DHO_DHase_2. IPR001295. Dihydroorotate_DHase_core. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_AZOC5

Accession

Primary (citable) accession number: A8HZX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 4, 2007
Last modified:	November 25, 2008
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)