Reviewed, UniProtKB/Swiss-Prot A8HZ95 (MURC_AZOC5)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	AZC_4556

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000071094
Regions
	Nucleotide binding	114 – 120	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A8HZ95-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: 1C4EF1B81BB4DDFC	FASTA	467	50,013
	10 20 30 40 50 60 MKLPEALGSI HFVGIGGIGM SGIAEVLNNL GYTVQGSDVA ENANVKRLRE NGIAVTVGHK 70 80 90 100 110 120 AENIDGAEVV VVSSAIKRDN PELLAARAKR LPVVRRAEML AELMRLKSCV AIAGTHGKTT 130 140 150 160 170 180 TTSLVATLLD KGGFDPTVIN GGIINAYGTN ARLGAGDWMV VEADESDGTF LKLPAEVAII 190 200 210 220 230 240 TNIDPEHLDH FKTFDKVQEA FRTFVENVPF YGFAVMCIDH PVVQALVGRI EDRRIITYGE 250 260 270 280 290 300 NPQADVRLVD VDLRGGITRF GVVFRDRAGT AEHRIDGLKL PMPGKHNALN ATAAIAVARE 310 320 330 340 350 360 LRVPDDRIIE AIGGFGGVKR RFTRTGEWKG ATIFDDYGHH PVEISAVLRA ARAATEGQVI 370 380 390 400 410 420 AVVQPHRYTR LASLFDEFCT CFNDADHVIV APVYAAGESP VPGADSEHLV QGLRARGHRS 430 440 450 460 VTALKGPEEL AGLVAGLAKP GDFVICLGAG TITQWAYALP GELEKLG

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP009384 Genomic DNA. Translation: BAF90554.1.
RefSeq	YP_001527472.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5691773.
GenomeReviews	Gene locus AZC_4556 in contig AP009384_GR.
KEGG	azc:AZC_4556.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EWMVVEA.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_AZOC5

Accession

Primary (citable) accession number: A8HZ95

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 4, 2007
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)