Reviewed, UniProtKB/Swiss-Prot A8HZ57 (MRAY_AZOC5)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	AZC_4541

Organism

Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP]

Taxonomic identifier

438753 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Azorhizobium

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity.
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_1000071045
Regions
	Transmembrane	27 – 47	21	Potential
	Transmembrane	72 – 92	21	Potential
	Transmembrane	94 – 114	21	Potential
	Transmembrane	133 – 153	21	Potential
	Transmembrane	169 – 189	21	Potential
	Transmembrane	200 – 220	21	Potential
	Transmembrane	237 – 257	21	Potential
	Transmembrane	264 – 284	21	Potential
	Transmembrane	289 – 309	21	Potential
	Transmembrane	338 – 358	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	A8HZ57-1 [UniParc]. Last modified December 4, 2007. Version 1. Checksum: E5F1736A49A65503	FASTA	361	38,603
	10 20 30 40 50 60 MLQWLAQLHD TFPAFNVFRY ITFRTGGAIV TAVLFVFLFG PGIISTLRLK QGKGQPIRAD 70 80 90 100 110 120 GPQSHLLTKK GTPTMGGLMI FSGLIVATLL WANLSNLYVW VVLFVTTGFG LIGFYDDYLK 130 140 150 160 170 180 VTRQSHAGFS GKARLAIEAL IAGIAVVLMI NAGRAGLSSS VAFPFFKDLL LDLGWFFVVF 190 200 210 220 230 240 GAFVIVAAGN AVNLTDGLDG LAIVPVMIAA ASFGMISYLS GNVVFADYLQ IHYVAGVGEL 250 260 270 280 290 300 AVICGAIIGA GLGFLWFNAP PAQIFMGDTG SLALGGLLGS IAVATKHEIV LAVIGGLFVL 310 320 330 340 350 360 EAVSVIVQVI SFRLTGKRVF RMAPIHHHFE QLGWTESQVV IRFWIIAVVL ALLGLATLKL R

References

[1]

"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571." Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP009384 Genomic DNA. Translation: BAF90539.1.
RefSeq	YP_001527457.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5687919.
GenomeReviews	Gene locus AZC_4541 in contig AP009384_GR.
KEGG	azc:AZC_4541.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MIAANFL.
Family and domain databases
HAMAP	MF_00038. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. 1 hit. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_AZOC5

Accession

Primary (citable) accession number: A8HZ57

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 4, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)