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Protein

Cyclic pyranopterin monophosphate synthase

Gene

moaA

Organism
Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes, together with MoaC, the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP or molybdopterin precursor Z).UniRule annotationSAAS annotation

Catalytic activityi

GTP = cyclic pyranopterin phosphate + diphosphate.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • S-adenosyl-L-methionineUniRule annotationSAAS annotationNote: Binds 1 S-adenosyl-L-methionine per subunit.UniRule annotationSAAS annotation
  • [4Fe-4S] clusterUniRule annotationSAAS annotationNote: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.UniRule annotationSAAS annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei83 – 831GTPUniRule annotation
Metal bindingi90 – 901Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi94 – 941Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Binding sitei96 – 961S-adenosyl-L-methionineUniRule annotation
Metal bindingi97 – 971Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Binding sitei132 – 1321GTPUniRule annotation
Binding sitei136 – 1361S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei166 – 1661GTPUniRule annotation
Binding sitei190 – 1901S-adenosyl-L-methionineUniRule annotation
Binding sitei226 – 2261GTPUniRule annotation
Binding sitei260 – 2601S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi323 – 3231Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation
Metal bindingi326 – 3261Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation
Metal bindingi340 – 3401Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. cyclic pyranopterin monophosphate synthase activity Source: UniProtKB-EC
  3. GTP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationSAAS annotation

Keywords - Biological processi

Molybdenum cofactor biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

4Fe-4SUniRule annotationSAAS annotation, GTP-bindingUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciACAU438753:GJF3-913-MONOMER.
UniPathwayiUPA00344.
UPA00344.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic pyranopterin monophosphate synthaseUniRule annotation (EC:4.1.99.18UniRule annotation)
Alternative name(s):
Molybdenum cofactor biosynthesis protein AUniRule annotation
Gene namesi
Name:moaAUniRule annotation
Ordered Locus Names:AZC_0899Imported
OrganismiAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571)Imported
Taxonomic identifieri438753 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeAzorhizobium
ProteomesiUP000000270: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. molybdopterin synthase complex Source: InterPro
Complete GO annotation...

Interactioni

Subunit structurei

Monomer and homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi438753.AZC_0899.

Structurei

3D structure databases

ProteinModelPortaliA8HU25.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni328 – 3303GTP bindingUniRule annotation

Sequence similaritiesi

Belongs to the radical SAM superfamily. MoaA family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2896.
HOGENOMiHOG000228680.
KOiK03639.
OMAiTREMHDR.
OrthoDBiEOG64XXNN.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8HU25-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSVPAGCPF AGSLQPPAYQ TAPCIRYKGD ARAGARPAGR ELSGCEEAGV
60 70 80 90 100
AFVTRDLGDR SGGSATALGP LVDTFGRAVS YLRVSVTDRC DFRCVYCMSE
110 120 130 140 150
HMTFLPKPDL LTLEELDRLC SAFVARGVRK LRLTGGEPLV RRDVMTLFRS
160 170 180 190 200
LSRHLETGRL EELTLTTNGS QLARFAGDLA ACGVKRINVS IDTLDPDKFR
210 220 230 240 250
AITRWGDLAK VLEGVKAAQR AGIHVKLNAV ALKDVNEHEI PSLMEWAHGE
260 270 280 290 300
GHDLSLIEVM PLGEVGAERV DQYLPLSKVR EDLATRYTLT DIDYRTGGPA
310 320 330 340 350
RYVSVKETGG RLGFITPMTH NFCEGCNRVR VTCTGTLYMC LGQDDAADLR
360 370 380 390
APLRASADDG VLQAAIDAAI FRKPKGHDFV IDRQTRQPAV SRHMSTTGG
Length:399
Mass (Da):43,426
Last modified:December 4, 2007 - v1
Checksum:i8BFFBE453552CA15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009384 Genomic DNA. Translation: BAF86897.1.
RefSeqiWP_012169430.1. NC_009937.1.
YP_001523815.1. NC_009937.1.

Genome annotation databases

EnsemblBacteriaiBAF86897; BAF86897; AZC_0899.
GeneIDi5688930.
KEGGiazc:AZC_0899.
PATRICi21018325. VBIAzoCau17976_0933.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009384 Genomic DNA. Translation: BAF86897.1.
RefSeqiWP_012169430.1. NC_009937.1.
YP_001523815.1. NC_009937.1.

3D structure databases

ProteinModelPortaliA8HU25.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi438753.AZC_0899.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF86897; BAF86897; AZC_0899.
GeneIDi5688930.
KEGGiazc:AZC_0899.
PATRICi21018325. VBIAzoCau17976_0933.

Phylogenomic databases

eggNOGiCOG2896.
HOGENOMiHOG000228680.
KOiK03639.
OMAiTREMHDR.
OrthoDBiEOG64XXNN.

Enzyme and pathway databases

UniPathwayiUPA00344.
UPA00344.
BioCyciACAU438753:GJF3-913-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571."
    Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43989 / DSM 5975 / ORS 571Imported.

Entry informationi

Entry nameiA8HU25_AZOC5
AccessioniPrimary (citable) accession number: A8HU25
Entry historyi
Integrated into UniProtKB/TrEMBL: December 4, 2007
Last sequence update: December 4, 2007
Last modified: March 4, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.