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A8HU25

- A8HU25_AZOC5

UniProt

A8HU25 - A8HU25_AZOC5

Protein

Cyclic pyranopterin monophosphate synthase

Gene

moaA

Organism
Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Catalyzes, together with MoaC, the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP or molybdopterin precursor Z).UniRule annotationSAAS annotation

    Catalytic activityi

    GTP = cyclic pyranopterin phosphate + diphosphate.UniRule annotationSAAS annotation

    Cofactori

    Binds 1 S-adenosyl-L-methionine per subunit.UniRule annotationSAAS annotation
    Binds 2 4Fe-4S clusters. Binds 1 4Fe-4S cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 4Fe-4S cluster coordinated with 3 cysteines and the GTP-derived substrate.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei83 – 831GTPUniRule annotation
    Metal bindingi90 – 901Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi94 – 941Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Binding sitei96 – 961S-adenosyl-L-methionineUniRule annotation
    Metal bindingi97 – 971Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Binding sitei132 – 1321GTPUniRule annotation
    Binding sitei136 – 1361S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei166 – 1661GTPUniRule annotation
    Binding sitei190 – 1901S-adenosyl-L-methionineUniRule annotation
    Binding sitei226 – 2261GTPUniRule annotation
    Binding sitei260 – 2601S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation
    Metal bindingi323 – 3231Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation
    Metal bindingi326 – 3261Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation
    Metal bindingi340 – 3401Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. cyclic pyranopterin monophosphate synthase activity Source: UniProtKB-EC
    3. GTP binding Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    LyaseUniRule annotationSAAS annotation

    Keywords - Biological processi

    Molybdenum cofactor biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    4Fe-4SUniRule annotationSAAS annotation, GTP-bindingUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionineUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciACAU438753:GJF3-913-MONOMER.
    UniPathwayiUPA00344.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclic pyranopterin monophosphate synthaseUniRule annotation (EC:4.1.99.18UniRule annotation)
    Alternative name(s):
    Molybdenum cofactor biosynthesis protein AUniRule annotation
    Gene namesi
    Name:moaAUniRule annotation
    Ordered Locus Names:AZC_0899Imported
    OrganismiAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571)Imported
    Taxonomic identifieri438753 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeAzorhizobium
    ProteomesiUP000000270: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. molybdopterin synthase complex Source: InterPro

    Interactioni

    Subunit structurei

    Monomer and homodimer.UniRule annotationSAAS annotation

    Protein-protein interaction databases

    STRINGi438753.AZC_0899.

    Structurei

    3D structure databases

    ProteinModelPortaliA8HU25.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni328 – 3303GTP bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. MoaA family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2896.
    HOGENOMiHOG000228680.
    KOiK03639.
    OMAiFRGLSRH.
    OrthoDBiEOG64XXNN.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01225_B. MoaA_B.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR013483. MoaA.
    IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
    IPR010505. Mob_synth_C.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06463. Mob_synth_C. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02666. moaA. 1 hit.
    PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8HU25-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSVPAGCPF AGSLQPPAYQ TAPCIRYKGD ARAGARPAGR ELSGCEEAGV    50
    AFVTRDLGDR SGGSATALGP LVDTFGRAVS YLRVSVTDRC DFRCVYCMSE 100
    HMTFLPKPDL LTLEELDRLC SAFVARGVRK LRLTGGEPLV RRDVMTLFRS 150
    LSRHLETGRL EELTLTTNGS QLARFAGDLA ACGVKRINVS IDTLDPDKFR 200
    AITRWGDLAK VLEGVKAAQR AGIHVKLNAV ALKDVNEHEI PSLMEWAHGE 250
    GHDLSLIEVM PLGEVGAERV DQYLPLSKVR EDLATRYTLT DIDYRTGGPA 300
    RYVSVKETGG RLGFITPMTH NFCEGCNRVR VTCTGTLYMC LGQDDAADLR 350
    APLRASADDG VLQAAIDAAI FRKPKGHDFV IDRQTRQPAV SRHMSTTGG 399
    Length:399
    Mass (Da):43,426
    Last modified:December 4, 2007 - v1
    Checksum:i8BFFBE453552CA15
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009384 Genomic DNA. Translation: BAF86897.1.
    RefSeqiYP_001523815.1. NC_009937.1.

    Genome annotation databases

    EnsemblBacteriaiBAF86897; BAF86897; AZC_0899.
    GeneIDi5688930.
    KEGGiazc:AZC_0899.
    PATRICi21018325. VBIAzoCau17976_0933.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009384 Genomic DNA. Translation: BAF86897.1 .
    RefSeqi YP_001523815.1. NC_009937.1.

    3D structure databases

    ProteinModelPortali A8HU25.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 438753.AZC_0899.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAF86897 ; BAF86897 ; AZC_0899 .
    GeneIDi 5688930.
    KEGGi azc:AZC_0899.
    PATRICi 21018325. VBIAzoCau17976_0933.

    Phylogenomic databases

    eggNOGi COG2896.
    HOGENOMi HOG000228680.
    KOi K03639.
    OMAi FRGLSRH.
    OrthoDBi EOG64XXNN.

    Enzyme and pathway databases

    UniPathwayi UPA00344 .
    BioCyci ACAU438753:GJF3-913-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01225_B. MoaA_B.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR013483. MoaA.
    IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
    IPR010505. Mob_synth_C.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06463. Mob_synth_C. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02666. moaA. 1 hit.
    PROSITEi PS01305. MOAA_NIFB_PQQE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571."
      Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.
      Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43989 / DSM 5975 / ORS 571Imported.

    Entry informationi

    Entry nameiA8HU25_AZOC5
    AccessioniPrimary (citable) accession number: A8HU25
    Entry historyi
    Integrated into UniProtKB/TrEMBL: December 4, 2007
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3