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A8HU25

- A8HU25_AZOC5

UniProt

A8HU25 - A8HU25_AZOC5

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Protein

Cyclic pyranopterin monophosphate synthase

Gene
moaA, AZC_0899
Organism
Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes, together with MoaC, the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP or molybdopterin precursor Z) By similarity.UniRule annotationSAAS annotations

Catalytic activityi

GTP = cyclic pyranopterin phosphate + diphosphate.UniRule annotationSAAS annotations

Cofactori

Binds 1 S-adenosyl-L-methionine per subunit By similarity.UniRule annotationSAAS annotations
Binds 2 4Fe-4S clusters. Binds 1 4Fe-4S cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 4Fe-4S cluster coordinated with 3 cysteines and the GTP-derived substrate By similarity.UniRule annotationSAAS annotations

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei83 – 831GTP By similarityUniRule annotation
Metal bindingi90 – 901Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarityUniRule annotation
Metal bindingi94 – 941Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarityUniRule annotation
Binding sitei96 – 961S-adenosyl-L-methionine By similarityUniRule annotation
Metal bindingi97 – 971Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarityUniRule annotation
Binding sitei132 – 1321GTP By similarityUniRule annotation
Binding sitei136 – 1361S-adenosyl-L-methionine; via carbonyl oxygen By similarityUniRule annotation
Binding sitei166 – 1661GTP By similarityUniRule annotation
Binding sitei190 – 1901S-adenosyl-L-methionine By similarityUniRule annotation
Binding sitei226 – 2261GTP By similarityUniRule annotation
Binding sitei260 – 2601S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarityUniRule annotation
Metal bindingi323 – 3231Iron-sulfur 2 (4Fe-4S-substrate) By similarityUniRule annotation
Metal bindingi326 – 3261Iron-sulfur 2 (4Fe-4S-substrate) By similarityUniRule annotation
Metal bindingi340 – 3401Iron-sulfur 2 (4Fe-4S-substrate) By similarityUniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. cyclic pyranopterin monophosphate synthase activity Source: UniProtKB-EC
  3. GTP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationSAAS annotations

Keywords - Biological processi

Molybdenum cofactor biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

4Fe-4SUniRule annotationSAAS annotations, GTP-bindingUniRule annotationSAAS annotations, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionineUniRule annotationSAAS annotations

Enzyme and pathway databases

BioCyciACAU438753:GJF3-913-MONOMER.
UniPathwayiUPA00344.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic pyranopterin monophosphate synthaseUniRule annotation (EC:4.1.99.18UniRule annotation)
Alternative name(s):
Molybdenum cofactor biosynthesis protein A
Gene namesi
Name:moaAUniRule annotation
Ordered Locus Names:AZC_0899Imported
OrganismiAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571)Imported
Taxonomic identifieri438753 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeAzorhizobium
ProteomesiUP000000270: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. molybdopterin synthase complex Source: InterPro
Complete GO annotation...

Interactioni

Subunit structurei

Monomer and homodimer By similarity.UniRule annotationSAAS annotations

Protein-protein interaction databases

STRINGi438753.AZC_0899.

Structurei

3D structure databases

ProteinModelPortaliA8HU25.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni328 – 3303GTP binding By similarityUniRule annotation

Sequence similaritiesi

Belongs to the radical SAM superfamily. MoaA family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2896.
HOGENOMiHOG000228680.
KOiK03639.
OMAiFRGLSRH.
OrthoDBiEOG64XXNN.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01225_B. MoaA_B.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamiPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02666. moaA. 1 hit.
PROSITEiPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8HU25-1 [UniParc]FASTAAdd to Basket

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MNSVPAGCPF AGSLQPPAYQ TAPCIRYKGD ARAGARPAGR ELSGCEEAGV    50
AFVTRDLGDR SGGSATALGP LVDTFGRAVS YLRVSVTDRC DFRCVYCMSE 100
HMTFLPKPDL LTLEELDRLC SAFVARGVRK LRLTGGEPLV RRDVMTLFRS 150
LSRHLETGRL EELTLTTNGS QLARFAGDLA ACGVKRINVS IDTLDPDKFR 200
AITRWGDLAK VLEGVKAAQR AGIHVKLNAV ALKDVNEHEI PSLMEWAHGE 250
GHDLSLIEVM PLGEVGAERV DQYLPLSKVR EDLATRYTLT DIDYRTGGPA 300
RYVSVKETGG RLGFITPMTH NFCEGCNRVR VTCTGTLYMC LGQDDAADLR 350
APLRASADDG VLQAAIDAAI FRKPKGHDFV IDRQTRQPAV SRHMSTTGG 399
Length:399
Mass (Da):43,426
Last modified:December 4, 2007 - v1
Checksum:i8BFFBE453552CA15
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009384 Genomic DNA. Translation: BAF86897.1.
RefSeqiYP_001523815.1. NC_009937.1.

Genome annotation databases

EnsemblBacteriaiBAF86897; BAF86897; AZC_0899.
GeneIDi5688930.
KEGGiazc:AZC_0899.
PATRICi21018325. VBIAzoCau17976_0933.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009384 Genomic DNA. Translation: BAF86897.1 .
RefSeqi YP_001523815.1. NC_009937.1.

3D structure databases

ProteinModelPortali A8HU25.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 438753.AZC_0899.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAF86897 ; BAF86897 ; AZC_0899 .
GeneIDi 5688930.
KEGGi azc:AZC_0899.
PATRICi 21018325. VBIAzoCau17976_0933.

Phylogenomic databases

eggNOGi COG2896.
HOGENOMi HOG000228680.
KOi K03639.
OMAi FRGLSRH.
OrthoDBi EOG64XXNN.

Enzyme and pathway databases

UniPathwayi UPA00344 .
BioCyci ACAU438753:GJF3-913-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01225_B. MoaA_B.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02666. moaA. 1 hit.
PROSITEi PS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571."
    Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43989 / DSM 5975 / ORS 571.

Entry informationi

Entry nameiA8HU25_AZOC5
AccessioniPrimary (citable) accession number: A8HU25
Entry historyi
Integrated into UniProtKB/TrEMBL: December 4, 2007
Last sequence update: December 4, 2007
Last modified: June 11, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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