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A8HU25 (A8HU25_AZOC5) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Cyclic pyranopterin monophosphate synthase HAMAP-Rule MF_01225

EC=4.1.99.18 HAMAP-Rule MF_01225
Alternative name(s):
Molybdenum cofactor biosynthesis protein A HAMAP-Rule MF_01225
Gene names
Name:moaA HAMAP-Rule MF_01225
Ordered Locus Names:AZC_0899 EMBL BAF86897.1
OrganismAzorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) [Complete proteome] [HAMAP] EMBL BAF86897.1
Taxonomic identifier438753 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeAzorhizobium

Protein attributes

Sequence length399 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes, together with MoaC, the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP or molybdopterin precursor Z) By similarity. HAMAP-Rule MF_01225 SAAS SAAS013483

Catalytic activity

GTP = cyclic pyranopterin phosphate + diphosphate. HAMAP-Rule MF_01225 SAAS SAAS013483

Cofactor

Binds 1 S-adenosyl-L-methionine per subunit By similarity. HAMAP-Rule MF_01225 SAAS SAAS013483

Binds 2 4Fe-4S clusters. Binds 1 4Fe-4S cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 4Fe-4S cluster coordinated with 3 cysteines and the GTP-derived substrate By similarity. HAMAP-Rule MF_01225 SAAS SAAS013483

Pathway

Cofactor biosynthesis; molybdopterin biosynthesis. HAMAP-Rule MF_01225 SAAS SAAS013483

Subunit structure

Monomer and homodimer By similarity. HAMAP-Rule MF_01225 SAAS SAAS013483

Sequence similarities

Belongs to the radical SAM superfamily. MoaA family. HAMAP-Rule MF_01225

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region328 – 3303GTP binding By similarity HAMAP-Rule MF_01225

Sites

Metal binding901Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_01225
Metal binding941Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_01225
Metal binding971Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_01225
Metal binding3231Iron-sulfur 2 (4Fe-4S-substrate) By similarity HAMAP-Rule MF_01225
Metal binding3261Iron-sulfur 2 (4Fe-4S-substrate) By similarity HAMAP-Rule MF_01225
Metal binding3401Iron-sulfur 2 (4Fe-4S-substrate) By similarity HAMAP-Rule MF_01225
Binding site831GTP By similarity HAMAP-Rule MF_01225
Binding site961S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01225
Binding site1321GTP By similarity HAMAP-Rule MF_01225
Binding site1361S-adenosyl-L-methionine; via carbonyl oxygen By similarity HAMAP-Rule MF_01225
Binding site1661GTP By similarity HAMAP-Rule MF_01225
Binding site1901S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01225
Binding site2261GTP By similarity HAMAP-Rule MF_01225
Binding site2601S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity HAMAP-Rule MF_01225

Sequences

Sequence LengthMass (Da)Tools
A8HU25 [UniParc].

Last modified December 4, 2007. Version 1.
Checksum: 8BFFBE453552CA15

FASTA39943,426
        10         20         30         40         50         60 
MNSVPAGCPF AGSLQPPAYQ TAPCIRYKGD ARAGARPAGR ELSGCEEAGV AFVTRDLGDR 

        70         80         90        100        110        120 
SGGSATALGP LVDTFGRAVS YLRVSVTDRC DFRCVYCMSE HMTFLPKPDL LTLEELDRLC 

       130        140        150        160        170        180 
SAFVARGVRK LRLTGGEPLV RRDVMTLFRS LSRHLETGRL EELTLTTNGS QLARFAGDLA 

       190        200        210        220        230        240 
ACGVKRINVS IDTLDPDKFR AITRWGDLAK VLEGVKAAQR AGIHVKLNAV ALKDVNEHEI 

       250        260        270        280        290        300 
PSLMEWAHGE GHDLSLIEVM PLGEVGAERV DQYLPLSKVR EDLATRYTLT DIDYRTGGPA 

       310        320        330        340        350        360 
RYVSVKETGG RLGFITPMTH NFCEGCNRVR VTCTGTLYMC LGQDDAADLR APLRASADDG 

       370        380        390 
VLQAAIDAAI FRKPKGHDFV IDRQTRQPAV SRHMSTTGG 

« Hide

References

[1]"Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium caulinodans ORS571."
Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T., Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B., Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43989 / DSM 5975 / ORS 571.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009384 Genomic DNA. Translation: BAF86897.1.
RefSeqYP_001523815.1. NC_009937.1.

3D structure databases

ProteinModelPortalA8HU25.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING438753.AZC_0899.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF86897; BAF86897; AZC_0899.
GeneID5688930.
KEGGazc:AZC_0899.
PATRIC21018325. VBIAzoCau17976_0933.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2896.
HOGENOMHOG000228680.
KOK03639.
OMAFRGLSRH.
OrthoDBEOG64XXNN.

Enzyme and pathway databases

BioCycACAU438753:GJF3-913-MONOMER.
UniPathwayUPA00344.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01225_B. MoaA_B.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR013483. MoaA.
IPR000385. MoaA_NifB_PqqE_Fe-S-bd_CS.
IPR010505. Mob_synth_C.
IPR007197. rSAM.
[Graphical view]
PfamPF06463. Mob_synth_C. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR02666. moaA. 1 hit.
PROSITEPS01305. MOAA_NIFB_PQQE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA8HU25_AZOC5
AccessionPrimary (citable) accession number: A8HU25
Entry history
Integrated into UniProtKB/TrEMBL: December 4, 2007
Last sequence update: December 4, 2007
Last modified: June 11, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)