A8HAL1 (UBP16_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 16
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 16
Ubiquitin thioesterase 16
Ubiquitin-specific-processing protease 16

Gene names

Name:	usp16
ORF Names:	si:dkey-121n8.2

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	815 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Specifically deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-10' of histone H3, and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Homotetramer By similarity.
Subcellular location	Nucleus By similarity.
Domain	The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin By similarity.
Sequence similarities	Belongs to the peptidase C19 family. USP16 subfamily. Contains 1 UBP-type zinc finger.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Activator Chromatin regulator Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW histone H2A K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB mitosis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of translational elongation Inferred from sequence or structural similarity. Source: UniProtKB protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB response to DNA damage stimulus Inferred from sequence or structural similarity. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	cysteine-type endopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB histone binding Inferred from sequence or structural similarity. Source: UniProtKB transcription coactivator activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin binding Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 815	815	Ubiquitin carboxyl-terminal hydrolase 16		PRO_0000367505
Regions
	Zinc finger	46 – 126	81	UBP-type
Sites
	Active site	217	1	Nucleophile By similarity
	Active site	752	1	Proton acceptor By similarity
	Metal binding	24	1	Zinc 1 By similarity
	Metal binding	26	1	Zinc 1 By similarity
	Metal binding	48	1	Zinc 2 By similarity
	Metal binding	51	1	Zinc 2 By similarity
	Metal binding	76	1	Zinc 3 By similarity
	Metal binding	79	1	Zinc 3 By similarity
	Metal binding	84	1	Zinc 2 By similarity
	Metal binding	91	1	Zinc 2 By similarity
	Metal binding	95	1	Zinc 3 By similarity
	Metal binding	104	1	Zinc 3 By similarity
	Metal binding	117	1	Zinc 1 By similarity
	Metal binding	120	1	Zinc 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8HAL1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 50CF71DC500EE191	FASTA	815	90,450
	10 20 30 40 50 60 MGKKKVKDRS AGTDSSSETA GPSCTHIRKG TENSVLKKAC LNEHWSSCQD CEQDKPEEKQ 70 80 90 100 110 120 ILEDQTDGES PAVWMCLKCG HRGCGRSGNQ HAIKHYETPR SEPHCLVLSL DVWSVWCYIC 130 140 150 160 170 180 DDEVQYSSTG QLAQLITNIR KQVLTAPDKR NASKKSWKED ISVMNSAEQT QDEEKGKKGK 190 200 210 220 230 240 QKSSSKQEDS PKSHQSAAAG SSAVVSVRGL SNLGNTCFFN AVVQSLSQTQ YLRELLKQIA 250 260 270 280 290 300 EEKSSFSITP ALSSELDPLQ IQLERPGSLT LAMCQLMNEI QETKKGVVTP KELFTQVCKK 310 320 330 340 350 360 APRFKGFQQQ DSQELLRYLL DGMRAEEAKR VNSGILEALK SSGKNFEAEQ TKKIVKEYEK 370 380 390 400 410 420 DGAPKNFVDR VFGGAMSSTV MCKECKTVSL VTEMFLDLSL PVADEAYRKK NQKKAVQHRH 430 440 450 460 470 480 SVSDDGDQDT SSLANGNEDM PTGTGSKYQQ KKAKKQAKKQ AKNQRRQQKQ GGKVTLDAIT 490 500 510 520 530 540 NQSSTDPADS SMQTQTVSVN GSADAQPADT NQEDLSLEKH NEDQDDEEPE QEQAASVNNR 550 560 570 580 590 600 FTALSEDQTT EDIAEQVNED EDEIEQNCAE EEELVEELNT MSLTTPSEGD VENGEDTLED 610 620 630 640 650 660 VKEYTVVNRD PELAFRALAS RTAPVKQECS VESCLYQFTE VEHLTENNRL MCVTCTKQQP 670 680 690 700 710 720 GYKDGCKKAV YRDALKQMLI SDPPVVLTLH LKRFQQVAYS VCKVNRHVQF PQILDLAPFC 730 740 750 760 770 780 SLNCTGVKEG ETQVLYSLYG IVEHSGTMRS GHYTAYVKSR PSTHNCVQNG TAAASGDAEA 790 800 810 SKGSWFHISD SSVHPVPEAK VQSSQAYLLF YEKIS

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR854897 Genomic DNA. Translation: CAP09451.1.
IPI	IPI00851694.
RefSeq	NP_001139569.1. NM_001146097.1.
UniGene	Dr.99665.
3D structure databases
ProteinModelPortal	A8HAL1.
SMR	A8HAL1. Positions 22-142.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000085645; ENSDARP00000080080; ENSDARG00000060633.
GeneID	569700.
KEGG	dre:569700.
Organism-specific databases
CTD	10600.
ZFIN	ZDB-GENE-030131-4153. usp16.
Phylogenomic databases
eggNOG	COG5560.
GeneTree	ENSGT00690000102047.
HOGENOM	HOG000154755.
HOVERGEN	HBG062704.
KO	K11844.
OMA	CKNVAEE.
OrthoDB	EOG495ZRD.
Gene expression databases
ArrayExpress	A8HAL1.
Bgee	A8HAL1.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR013083. Znf_RING/FYVE/PHD. IPR001607. Znf_UBP. [Graphical view]
Pfam	PF00443. UCH. 1 hit. PF02148. zf-UBP. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20889807.

Entry information

Entry name

UBP16_DANRE

Accession

Primary (citable) accession number: A8HAL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 13, 2007
Last modified:	April 3, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program