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Protein

Ubiquitin carboxyl-terminal hydrolase 16

Gene

usp16

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.IEP:

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).IEP:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi24Zinc 1IEP:1
Metal bindingi26Zinc 1IEP:1
Metal bindingi48Zinc 2IEP:1
Metal bindingi51Zinc 2IEP:1
Metal bindingi76Zinc 3IEP:1
Metal bindingi79Zinc 3IEP:1
Metal bindingi84Zinc 2IEP:1
Metal bindingi91Zinc 2IEP:1
Metal bindingi95Zinc 3IEP:1
Metal bindingi104Zinc 3IEP:1
Metal bindingi117Zinc 1IEP:1
Metal bindingi120Zinc 1IEP:1
Active sitei217NucleophileIEP:1
Active sitei752Proton acceptorIEP:1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri46 – 126UBP-typeIEP:Add BLAST81

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Chromatin regulator, Hydrolase, Protease, Thiol protease
Biological processCell cycle, Cell division, Mitosis, Transcription, Transcription regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 16IEP: (EC:3.4.19.12IEP:)
Alternative name(s):
Deubiquitinating enzyme 16IEP:
Ubiquitin thioesterase 16IEP:
Ubiquitin-specific-processing protease 16IEP:
Gene namesi
Name:usp16
ORF Names:si:dkey-121n8.2
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 15

Organism-specific databases

ZFINiZDB-GENE-030131-4153. usp16.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003675051 – 815Ubiquitin carboxyl-terminal hydrolase 16Add BLAST815

Proteomic databases

PaxDbiA8HAL1.
PeptideAtlasiA8HAL1.

Expressioni

Gene expression databases

BgeeiENSDARG00000060633.
ExpressionAtlasiA8HAL1. baseline.

Interactioni

Subunit structurei

Homotetramer.IEP:

GO - Molecular functioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000080080.

Structurei

3D structure databases

ProteinModelPortaliA8HAL1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini208 – 814USPAdd BLAST607

Domaini

The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin.IEP:

Sequence similaritiesi

Belongs to the peptidase C19 family. USP16 subfamily.IEP:

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri46 – 126UBP-typeIEP:Add BLAST81

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1873. Eukaryota.
COG5560. LUCA.
GeneTreeiENSGT00860000133682.
HOGENOMiHOG000154755.
HOVERGENiHBG062704.
InParanoidiA8HAL1.
KOiK11844.
OMAiNTYECEK.
OrthoDBiEOG091G01WT.
PhylomeDBiA8HAL1.
TreeFamiTF326075.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
HAMAPiMF_03062. UBP16. 1 hit.
InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR030849. UBP16.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
PfamiView protein in Pfam
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
SMARTiView protein in SMART
SM00290. ZnF_UBP. 1 hit.
PROSITEiView protein in PROSITE
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.

Sequencei

Sequence statusi: Complete.

A8HAL1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKKVKDRS AGTDSSSETA GPSCTHIRKG TENSVLKKAC LNEHWSSCQD
60 70 80 90 100
CEQDKPEEKQ ILEDQTDGES PAVWMCLKCG HRGCGRSGNQ HAIKHYETPR
110 120 130 140 150
SEPHCLVLSL DVWSVWCYIC DDEVQYSSTG QLAQLITNIR KQVLTAPDKR
160 170 180 190 200
NASKKSWKED ISVMNSAEQT QDEEKGKKGK QKSSSKQEDS PKSHQSAAAG
210 220 230 240 250
SSAVVSVRGL SNLGNTCFFN AVVQSLSQTQ YLRELLKQIA EEKSSFSITP
260 270 280 290 300
ALSSELDPLQ IQLERPGSLT LAMCQLMNEI QETKKGVVTP KELFTQVCKK
310 320 330 340 350
APRFKGFQQQ DSQELLRYLL DGMRAEEAKR VNSGILEALK SSGKNFEAEQ
360 370 380 390 400
TKKIVKEYEK DGAPKNFVDR VFGGAMSSTV MCKECKTVSL VTEMFLDLSL
410 420 430 440 450
PVADEAYRKK NQKKAVQHRH SVSDDGDQDT SSLANGNEDM PTGTGSKYQQ
460 470 480 490 500
KKAKKQAKKQ AKNQRRQQKQ GGKVTLDAIT NQSSTDPADS SMQTQTVSVN
510 520 530 540 550
GSADAQPADT NQEDLSLEKH NEDQDDEEPE QEQAASVNNR FTALSEDQTT
560 570 580 590 600
EDIAEQVNED EDEIEQNCAE EEELVEELNT MSLTTPSEGD VENGEDTLED
610 620 630 640 650
VKEYTVVNRD PELAFRALAS RTAPVKQECS VESCLYQFTE VEHLTENNRL
660 670 680 690 700
MCVTCTKQQP GYKDGCKKAV YRDALKQMLI SDPPVVLTLH LKRFQQVAYS
710 720 730 740 750
VCKVNRHVQF PQILDLAPFC SLNCTGVKEG ETQVLYSLYG IVEHSGTMRS
760 770 780 790 800
GHYTAYVKSR PSTHNCVQNG TAAASGDAEA SKGSWFHISD SSVHPVPEAK
810
VQSSQAYLLF YEKIS
Length:815
Mass (Da):90,450
Last modified:November 13, 2007 - v1
Checksum:i50CF71DC500EE191
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR854897 Genomic DNA. Translation: CAP09451.1.
RefSeqiNP_001139569.1. NM_001146097.1.
XP_009290187.1. XM_009291912.2.
UniGeneiDr.24329.
Dr.99665.

Genome annotation databases

EnsembliENSDART00000085645; ENSDARP00000080080; ENSDARG00000060633.
GeneIDi569700.
KEGGidre:569700.

Similar proteinsi

Entry informationi

Entry nameiUBP16_DANRE
AccessioniPrimary (citable) accession number: A8HAL1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 13, 2007
Last modified: November 22, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families