Reviewed, UniProtKB/Swiss-Prot A8HAF6 (GUAC_SHEPA)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	Spea_4233

Organism

Shewanella pealeana (strain ATCC 700345 / ANG-SQ1) [Complete proteome] [HAMAP]

Taxonomic identifier

398579 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH(3) + NADP(+) = guanosine 5'-phosphate + NADPH.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 1 subfamily.

Ontologies

Keywords
Ligand	Metal-binding NADP Potassium
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	GMP reductase		PRO_1000082442
Regions
	Nucleotide binding	108 – 131	24	NADP By similarity
	Nucleotide binding	216 – 239	24	NADP; ribose moiety By similarity
Sites
	Active site	186	1	Thioimidate intermediate By similarity
	Metal binding	181	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	183	1	Potassium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8HAF6-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 2F0CE4113BD5832B	FASTA	347	37,061
	10 20 30 40 50 60 MRIEQDLKLG FKDVLIRPKR STLKSRSQVD LNRQFTFKHS GKTWSGVPII AANMDSVASF 70 80 90 100 110 120 EMAASLAQHN VMTAVHKHYS VEQWGEFVAS QTAEVLQHVM VSSGTSDTDF IKLSEILAKS 130 140 150 160 170 180 EDLNFICIDI ANGYSEHLVD YVRKVRQAHP QAVISAGNVV TGDMVEELII AGADIVKVGI 190 200 210 220 230 240 GPGSVCTTRV KTGVGYPQLS AIIECADAAH GLGGQIIGDG GCSCAGDVAK AFGGGADFVM 250 260 270 280 290 300 LGGMLAGHEQ SGGEVVEQDG KMMVKFYGMS SQSAMDKHSG GVAKYRAAEG KTVLLPFKGS 310 320 330 340 VDNTINDIMG GVRSTCTYVG AASLKELTKR TTFIRVQEQE NNVYGKE

References

[1]

"Complete sequence of Shewanella pealeana ATCC 700345." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Zhao J.-S.Z., Manno D., Hawari J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000851 Genomic DNA. Translation: ABV89543.1.
RefSeq	YP_001504078.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5664617.
GenomeReviews	Gene locus Spea_4233 in contig CP000851_GR.
KEGG	spl:Spea_4233.
NMPDR	fig|398579.3.peg.3674.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00596. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005993. GMP_reduct1. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DHase_GMPRtase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF000235. GMP_reductase. 1 hit.
TIGRFAMs	TIGR01305. GMP_reduct_1. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_SHEPA

Accession

Primary (citable) accession number: A8HAF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 13, 2007
Last modified:	November 4, 2008
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)