Reviewed, UniProtKB/Swiss-Prot A8H8E0 (ASTD_SHEPA)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	Spea_3514

Organism

Shewanella pealeana (strain ATCC 700345 / ANG-SQ1) [Complete proteome] [HAMAP]

Taxonomic identifier

398579 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD(+) + H(2)O = N-succinyl-L-glutamate + NADH.
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5.
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	N-succinylglutamate 5-semialdehyde dehydrogenase		PRO_1000085408
Regions
	Nucleotide binding	220 – 225	6	NAD By similarity
Sites
	Active site	243	1	By similarity
	Active site	277	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8H8E0-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 1D504D6C975E80A0	FASTA	486	51,660
	10 20 30 40 50 60 MTQFIEGKWV EGLGHEVTSI NPANQKVIWN SKTATPEQVG VAVDAARNAQ FDWFMLGFDA 70 80 90 100 110 120 RLAIVEAYRD QLEANKAEMA EVIAQETGKP QWETATEAGA MIGKIGLSVA AYHKRTGTSE 130 140 150 160 170 180 NDTPAGRAVL RHKPHGVVAV FGPYNFPGHL PNGHIVPALL AGNTVVFKPS ELTPKVAELM 190 200 210 220 230 240 LKLWEKAGLP KGVINLVQGE VETGKALAAH PQIDGLFFTG SSRTGHILHQ QYAGDPGKIL 250 260 270 280 290 300 ALEMGGNNPL IIKDVADTKA AVHDIIQSAY ISAGQRCTCA RRLYVEKGAA GDALLEQLVA 310 320 330 340 350 360 AVKQIQVGPW NAQPQPFMGS MISETAAKGM VEAQRNLVNL GGNALVELTH LEVGTGLVSP 370 380 390 400 410 420 GLIDVTGVIE LPDEEYFGPL LQVVRYSDFD EAIKLANATR YGLSAGILAD SREDYDYFLA 430 440 450 460 470 480 RIRAGIVNWN KQITGASGAA PFGGVGASGN HRASAFYAAD YCAYPVASVE ADAVSLPATL SPGLSI

References

[1]

"Complete sequence of Shewanella pealeana ATCC 700345." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Zhao J.-S.Z., Manno D., Hawari J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000851 Genomic DNA. Translation: ABV88827.1.
RefSeq	YP_001503362.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5663900.
GenomeReviews	Gene locus Spea_3514 in contig CP000851_GR.
KEGG	spl:Spea_3514.
NMPDR	fig|398579.3.peg.3029.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01174. [Tree]
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_SHEPA

Accession

Primary (citable) accession number: A8H8E0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 13, 2007
Last modified:	November 25, 2008
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)