A8H7G5 (MAO1_SHEPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAD-dependent malic enzyme
Short name=NAD-ME
EC=1.1.1.38

Gene names

Name:	maeA
Ordered Locus Names:	Spea_3186

Organism

Shewanella pealeana (strain ATCC 700345 / ANG-SQ1) [Complete proteome] [HAMAP]

Taxonomic identifier

398579 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity. HAMAP MF_01619
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	NAD-dependent malic enzyme HAMAP MF_01619		PRO_1000088076
Sites
	Active site	101	1	Proton donor By similarity
	Active site	172	1	Proton acceptor By similarity
	Metal binding	243	1	Divalent metal cation By similarity
	Metal binding	244	1	Divalent metal cation By similarity
	Metal binding	267	1	Divalent metal cation By similarity
	Binding site	154	1	NAD By similarity
	Binding site	267	1	NAD By similarity
	Binding site	415	1	NAD By similarity
	Site	267	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8H7G5 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 18FD5B188CC63DEF	FASTA	562	62,323
	10 20 30 40 50 60 MDDNKRPLYL PFAGPAILEA PLINKGSAFT DEERIFFNLE GLLPHVIETI EEQASRAYDQ 70 80 90 100 110 120 YKNFSNDLDK HIYLRNIQDT NETLYYRLVQ NHITEMMPII YTPTVGMACE RFSKDYRRNR 130 140 150 160 170 180 GLFISYANKD RIDDILNNST RQKVKIIVVT DGERILGLGD QGIGGMGIPI GKLSLYTSCG 190 200 210 220 230 240 GISPAYTLPI TLDVGTDNPH LLEDPMYMGM RSPRIGGEEY TEFVEAFMQA VHRRWPDALI 250 260 270 280 290 300 QFEDFAQKNA MPLLERYKDQ YCCFNDDIQG TAAVTVGSLL AACKAAKTQL SEQRITFLGA 310 320 330 340 350 360 GSAGCGIAEA IVAQMVSEGI SEAQARKQVF MVDRWGMLQS NMPNLLPFQQ KLAQNCDDIT 370 380 390 400 410 420 SWDNFSDNIS LLDVVNNAKP TVLIGVSGAP GLFTEEIIKA MHSHCKRPIV FPLSNPTSRV 430 440 450 460 470 480 EATPKDILHW TQGQALVATG SPFEPVVVDD VTYEIAQCNN SYIFPGIGLG VLASGAKRVS 490 500 510 520 530 540 DAMLMASSRA LAECSPLAID GEGSLLPKLE DIHKVSKHIA FAVARTAIEE GHALPTTNEL 550 560 LTYAIEDNFW TAEYRSYKRT AF

References

[1]

"Complete sequence of Shewanella pealeana ATCC 700345." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Zhao J.-S.Z., Manno D., Hawari J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700345 / ANG-SQ1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000851 Genomic DNA. Translation: ABV88502.1.
RefSeq	YP_001503037.1. NC_009901.1.
3D structure databases
ProteinModelPortal	A8H7G5.
ModBase	Search...
Protein-protein interaction databases
STRING	A8H7G5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5663575.
GenomeReviews	Gene locus Spea_3186 in contig CP000851_GR.
KEGG	spl:Spea_3186.
NMPDR	fig|398579.3.peg.2759.
PATRIC	23536507. VBIShePea72822_3332.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0281.
HOGENOM	HBG289821.
OMA	QDNNETL.
ProtClustDB	PRK13529.
Enzyme and pathway databases
BioCyc	SPEA398579:SPEA_3186-MONOMER.
Family and domain databases
HAMAP	MF_01619. NAD_malic_enz. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD-bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. IPR023667. NAD_malic_enz_proteobac. [Graphical view]
Gene3D	G3DSA:3.40.50.10380. G3DSA:3.40.50.10380. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00027.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PIRSF	PIRSF000106. ME. 1 hit.
PRINTS	PR00072. MALOXRDTASE.
SMART	SM00919. Malic_M. 1 hit. [Graphical view]
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_SHEPA

Accession

Primary (citable) accession number: A8H7G5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program