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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Shewanella pealeana (strain ATCC 700345 / ANG-SQ1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei621 – 6211ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPEA398579:GHG5-3301-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Spea_3143
OrganismiShewanella pealeana (strain ATCC 700345 / ANG-SQ1)
Taxonomic identifieri398579 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000002608 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 859859Leucine--tRNA ligasePRO_0000334819Add
BLAST

Proteomic databases

PRIDEiA8H7C2.

Interactioni

Protein-protein interaction databases

STRINGi398579.Spea_3143.

Structurei

3D structure databases

ProteinModelPortaliA8H7C2.
SMRiA8H7C2. Positions 228-412.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi618 – 6225"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8H7C2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEQYTPSEI EAKVQQHWQD TKTFEVTEDE NKEKFYCLSM FPYPSGRLHM
60 70 80 90 100
GHVRNYTIGD VVSRYQRLQG KNVLQPIGWD SFGLPAENAA IKNNTAPAPW
110 120 130 140 150
TYENIDYMKN QLKMLGFGYD WSREIATCTP EYYRWEQWFF TKLYEKGLVY
160 170 180 190 200
KKTSSVNWCP NDETVLANEQ VIDGCCWRCD TTVEQKEIPQ WFIKITEYAE
210 220 230 240 250
ELLNDIDTLE EWPEQVKTMQ RNWIGRSEGI EMTFQVAGSD QSFDIYTTRP
260 270 280 290 300
DTVMGVTYVA IAAGHPLAEQ AAINNPALVE FIEECKNADT TEAAMAAMEK
310 320 330 340 350
KGVATGLNAI HPITGKEVPI WVGNFVLMNY GTGAVMSVPA HDQRDYEFAK
360 370 380 390 400
KYGLNIEAVI KPVDGEVDIS EEAYTEKGVL FNSAEFDGLD FQAAFDAIDA
410 420 430 440 450
KLTAEGKGKR QVNFRLRDWG VSRQRYWGAP IPMVTLADGT VMPTPEDQLP
460 470 480 490 500
VILPEDVVMD GIQSPIKSDK EWAKTTINGQ EAFRETDTFD TFMESSWYYA
510 520 530 540 550
RYCSPHADEM LDPAKANYWL PVDQYIGGIE HACMHLLYFR FFHKLLRDTG
560 570 580 590 600
LVNSNEPAKR LLTQGMVLAD AYYYNNEKGA RVWVAPSDVT VQETDDKGRT
610 620 630 640 650
VKAVDSEGHE LVYTGMSKMS KSKNNGIDPQ EMVDKYGADT VRLFMMFAAP
660 670 680 690 700
PELTLEWQES SVEGAHRFIK RLWKTAHDHI ANGTTAELDV KSLNAAQKEL
710 720 730 740 750
RRELHKTIAK VGDDIERRQM FNTAIASIME LMNRLQKAPS ETDQDKALMQ
760 770 780 790 800
EALNAVIRLL YPIIPHTCFV LWNELGNQGA IEEVLWPEVD ESALVEDSKL
810 820 830 840 850
IIVQVNGKLR AKITVAADAS KEEVEAAGMA EEGVVKHTED KTVRKVIYVP

GKLLNIVAN
Length:859
Mass (Da):97,391
Last modified:May 20, 2008 - v2
Checksum:iBE2D4C6317A1466E
GO

Sequence cautioni

The sequence ABV88459.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000851 Genomic DNA. Translation: ABV88459.1. Different initiation.
RefSeqiYP_001502994.1. NC_009901.1.

Genome annotation databases

EnsemblBacteriaiABV88459; ABV88459; Spea_3143.
KEGGispl:Spea_3143.
PATRICi23536403. VBIShePea72822_3286.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000851 Genomic DNA. Translation: ABV88459.1. Different initiation.
RefSeqiYP_001502994.1. NC_009901.1.

3D structure databases

ProteinModelPortaliA8H7C2.
SMRiA8H7C2. Positions 228-412.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi398579.Spea_3143.

Proteomic databases

PRIDEiA8H7C2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV88459; ABV88459; Spea_3143.
KEGGispl:Spea_3143.
PATRICi23536403. VBIShePea72822_3286.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciSPEA398579:GHG5-3301-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700345 / ANG-SQ1.

Entry informationi

Entry nameiSYL_SHEPA
AccessioniPrimary (citable) accession number: A8H7C2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: April 1, 2015
This is version 52 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.