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A8H7C2 (SYL_SHEPA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Spea_3143
OrganismShewanella pealeana (strain ATCC 700345 / ANG-SQ1) [Complete proteome] [HAMAP]
Taxonomic identifier398579 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length859 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence ABV88459.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 859859Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334819

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif618 – 6225"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6211ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8H7C2 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: BE2D4C6317A1466E

FASTA85997,391
        10         20         30         40         50         60 
MQEQYTPSEI EAKVQQHWQD TKTFEVTEDE NKEKFYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VVSRYQRLQG KNVLQPIGWD SFGLPAENAA IKNNTAPAPW TYENIDYMKN QLKMLGFGYD 

       130        140        150        160        170        180 
WSREIATCTP EYYRWEQWFF TKLYEKGLVY KKTSSVNWCP NDETVLANEQ VIDGCCWRCD 

       190        200        210        220        230        240 
TTVEQKEIPQ WFIKITEYAE ELLNDIDTLE EWPEQVKTMQ RNWIGRSEGI EMTFQVAGSD 

       250        260        270        280        290        300 
QSFDIYTTRP DTVMGVTYVA IAAGHPLAEQ AAINNPALVE FIEECKNADT TEAAMAAMEK 

       310        320        330        340        350        360 
KGVATGLNAI HPITGKEVPI WVGNFVLMNY GTGAVMSVPA HDQRDYEFAK KYGLNIEAVI 

       370        380        390        400        410        420 
KPVDGEVDIS EEAYTEKGVL FNSAEFDGLD FQAAFDAIDA KLTAEGKGKR QVNFRLRDWG 

       430        440        450        460        470        480 
VSRQRYWGAP IPMVTLADGT VMPTPEDQLP VILPEDVVMD GIQSPIKSDK EWAKTTINGQ 

       490        500        510        520        530        540 
EAFRETDTFD TFMESSWYYA RYCSPHADEM LDPAKANYWL PVDQYIGGIE HACMHLLYFR 

       550        560        570        580        590        600 
FFHKLLRDTG LVNSNEPAKR LLTQGMVLAD AYYYNNEKGA RVWVAPSDVT VQETDDKGRT 

       610        620        630        640        650        660 
VKAVDSEGHE LVYTGMSKMS KSKNNGIDPQ EMVDKYGADT VRLFMMFAAP PELTLEWQES 

       670        680        690        700        710        720 
SVEGAHRFIK RLWKTAHDHI ANGTTAELDV KSLNAAQKEL RRELHKTIAK VGDDIERRQM 

       730        740        750        760        770        780 
FNTAIASIME LMNRLQKAPS ETDQDKALMQ EALNAVIRLL YPIIPHTCFV LWNELGNQGA 

       790        800        810        820        830        840 
IEEVLWPEVD ESALVEDSKL IIVQVNGKLR AKITVAADAS KEEVEAAGMA EEGVVKHTED 

       850 
KTVRKVIYVP GKLLNIVAN 

« Hide

References

[1]"Complete sequence of Shewanella pealeana ATCC 700345."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., Zhao J.-S.Z., Manno D., Hawari J., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700345 / ANG-SQ1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000851 Genomic DNA. Translation: ABV88459.1. Different initiation.
RefSeqYP_001502994.1. NC_009901.1.

3D structure databases

ProteinModelPortalA8H7C2.
SMRA8H7C2. Positions 228-412.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING398579.Spea_3143.

Proteomic databases

PRIDEA8H7C2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV88459; ABV88459; Spea_3143.
GeneID5663532.
KEGGspl:Spea_3143.
PATRIC23536403. VBIShePea72822_3286.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycSPEA398579:GHG5-3301-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_SHEPA
AccessionPrimary (citable) accession number: A8H7C2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: April 16, 2014
This is version 47 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries