A8H786 (RLMG_SHEPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	Spea_3107

Organism

Shewanella pealeana (strain ATCC 700345 / ANG-SQ1) [Complete proteome] [HAMAP]

Taxonomic identifier

398579 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.
Sequence caution	The sequence ABV88423.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366513

Sequences

	Sequence		Length	Mass (Da)
	A8H786 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: 8269BA2EA8FCBE94	FASTA	413	45,683
	10 20 30 40 50 60 MTTQFSVSGI ELELFRYPSR QESNLQAWDA ADEHLIKHLI DTEQTSISTA VVNDGFGALT 70 80 90 100 110 120 AGLLSINPSW PLTLETDAKT SQLGTSQNLT RNQLSQESIT WVNSRDELPQ VIELVLMKLP 130 140 150 160 170 180 KNLNYFSHQL NRLSHVLPAG TQVLIGAKAK SINKSLLETI EKNLGPASAS LTWKKTRVIT 190 200 210 220 230 240 CIADGKARAL PKATTWSVPE FKLQISNLSN VFAANKLDIG ARIMLDNMPK GDYKSIVDLG 250 260 270 280 290 300 CGNGILGLHA KQVFPEAYIH FIDDSEMAVA SARENWALNK LDNPALVGEQ ATFGWDDCLT 310 320 330 340 350 360 NMSEGFRPDL VLCNPPFHQG EAITDHIAWQ MFLDAFRRLK NGGILHVVGN RHLAYHVKLQ 370 380 390 400 410 RIFKNCTTVA SNGKFVILQA QKISKKAQEA EVEQAFDTET PHPQSALYGK PKA

References

[1]

"Complete sequence of Shewanella pealeana ATCC 700345." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Zhao J.-S.Z., Manno D., Hawari J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700345 / ANG-SQ1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000851 Genomic DNA. Translation: ABV88423.1. Different initiation.
RefSeq	YP_001502958.1. NC_009901.1.
3D structure databases
ProteinModelPortal	A8H786.
ModBase	Search...
Protein-protein interaction databases
STRING	A8H786.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5663496.
GenomeReviews	Gene locus Spea_3107 in contig CP000851_GR.
KEGG	spl:Spea_3107.
NMPDR	fig|398579.3.peg.2691.
PATRIC	23536303. VBIShePea72822_3249.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HBG321931.
ProtClustDB	CLSK906089.
Enzyme and pathway databases
BioCyc	SPEA398579:SPEA_3107-MONOMER.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K00564.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_SHEPA

Accession

Primary (citable) accession number: A8H786

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program