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A8H5E3 (A8H5E3_SHEPA) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Imidazole glycerol phosphate synthase subunit HisH HAMAP-Rule MF_00278
EC=2.4.2.- HAMAP-Rule MF_00278
Alternative name(s):
IGP synthase glutamine amidotransferase subunit HAMAP-Rule MF_00278
IGP synthase subunit HisH HAMAP-Rule MF_00278
ImGP synthase subunit HisH HAMAP-Rule MF_00278
Gene names
Name:hisH HAMAP-Rule MF_00278
Ordered Locus Names:Spea_2460 EMBL ABV87780.1
OrganismShewanella pealeana (strain ATCC 700345 / ANG-SQ1) [Complete proteome] [HAMAP] EMBL ABV87780.1
Taxonomic identifier398579 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella ›

Protein attributes

Sequence length247 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR By similarity. HAMAP-Rule MF_00278 SAAS SAAS010139

Catalytic activity

5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O. HAMAP-Rule MF_00278 SAAS SAAS010139

Pathway

Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9. HAMAP-Rule MF_00278 SAAS SAAS010139

Subunit structure

Heterodimer of HisH and HisF By similarity. HAMAP-Rule MF_00278 SAAS SAAS010139

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00278 SAAS SAAS010139.

Sequence similarities

Contains 1 glutamine amidotransferase type-1 domain. HAMAP-Rule MF_00278 SAAS SAAS010139

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain24 – 233210Glutamine amidotransferase type-1 By similarity HAMAP-Rule MF_00278

Sites

Active site1041Nucleophile By similarity HAMAP-Rule MF_00278
Active site2081 By similarity HAMAP-Rule MF_00278
Active site2101 By similarity HAMAP-Rule MF_00278

Sequences

Sequence LengthMass (Da)Tools
A8H5E3 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: 74C956E356FC6A0B

FASTA24726,921
        10         20         30         40         50         60 
MSQQDKAGLS DDRSLNNNKA IVGNTVIVDT GCANLSSVRY AFERLMTDIE RERLFVTDDI 

        70         80         90        100        110        120 
EMIRAAERVI LPGVGTAAAA MSALQSKGLV QLIPELTQPV LGVCLGMQML TRVSKERGSQ 

       130        140        150        160        170        180 
SEDCPCLNII PTDINLLDTK GLPLPHMGWN QLVPGSHPIF DGITAGSYVY FVHSYRAPIS 

       190        200        210        220        230        240 
DYTIATAQHG ETFSAAIAKD NFIGLQFHPE KSAEVGARML KNFLTMDQAT FSPTNNQLAY 


GQQEAQL

« Hide

References

[1]"Complete sequence of Shewanella pealeana ATCC 700345."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., Zhao J.-S.Z., Manno D., Hawari J., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700345 / ANG-SQ1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000851 Genomic DNA. Translation: ABV87780.1.
RefSeqYP_001502315.1. NC_009901.1.

3D structure databases

ProteinModelPortalA8H5E3.
ModBaseSearch...

Protein-protein interaction databases

STRING398579.Spea_2460.

Protein family/group databases

MEROPSC26.965.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV87780; ABV87780; Spea_2460.
GeneID5662853.
KEGGspl:Spea_2460.
PATRIC23534922. VBIShePea72822_2576.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0118.
HOGENOMHOG000025030.
KOK02501.
OMAYSEEDDI.
ProtClustDBPRK13170.

Enzyme and pathway databases

UniPathwayUPA00031; UER00010.

Family and domain databases

HAMAPMF_00278. HisH.
InterProIPR017926. GATASE_1.
IPR010139. Imidazole-glycPsynth_HisH.
[Graphical view]
PfamPF00117. GATase. 1 hit.
[Graphical view]
PIRSFPIRSF000495. Amidotransf_hisH. 1 hit.
TIGRFAMsTIGR01855. IMP_synth_hisH. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA8H5E3_SHEPA
AccessionPrimary (citable) accession number: A8H5E3
Entry history
Integrated into UniProtKB/TrEMBL: November 13, 2007
Last sequence update: November 13, 2007
Last modified: May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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