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A8H5A5 (DDL_SHEPA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Spea_2422
OrganismShewanella pealeana (strain ATCC 700345 / ANG-SQ1) [Complete proteome] [HAMAP]
Taxonomic identifier398579 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000074790

Regions

Domain124 – 329206ATP-grasp
Nucleotide binding154 – 20956ATP By similarity

Sites

Metal binding2831Magnesium or manganese 1 By similarity
Metal binding2961Magnesium or manganese 1 By similarity
Metal binding2961Magnesium or manganese 2 By similarity
Metal binding2981Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A8H5A5 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: EDC1BB08771F0D50

FASTA33437,223
        10         20         30         40         50         60 
MTQKNLLLLC GGGGDEHSIS LLSANFFEAQ LATLPHFNVL RVELNAKGQY HTQAGELCEL 

        70         80         90        100        110        120 
TNSQQLRFDD ETKAPWNVDY VIPCIHGYPG ETGDIQSYFE LINLPYFGCD SESSSNCFNK 

       130        140        150        160        170        180 
VTAKMWFSAL GIPNTPYIFL SEFNQQSLEQ AEAALAKWGS IFIKAASQGS SVGCYRVDSI 

       190        200        210        220        230        240 
EQLASSLEEA FTFSPYVVIE KTITARELEV AAYEADGEII ATKPGEIICA SNTFYSFDEK 

       250        260        270        280        290        300 
YAENSQAQTV IEADVDEAIA KQIQEYAIKA FKGMKLRHLS RIDFFLTEDN EILLNEINTF 

       310        320        330 
PGQTQISMFP KMLQNHGHNF AQYLSGNILA QLKS 

« Hide

References

[1]"Complete sequence of Shewanella pealeana ATCC 700345."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., Zhao J.-S.Z., Manno D., Hawari J., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700345 / ANG-SQ1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000851 Genomic DNA. Translation: ABV87742.1.
RefSeqYP_001502277.1. NC_009901.1.

3D structure databases

ProteinModelPortalA8H5A5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING398579.Spea_2422.

Proteomic databases

PRIDEA8H5A5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV87742; ABV87742; Spea_2422.
GeneID5662815.
KEGGspl:Spea_2422.
PATRIC23534844. VBIShePea72822_2537.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAMEFCVLF.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycSPEA398579:GHG5-2549-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_SHEPA
AccessionPrimary (citable) accession number: A8H5A5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 13, 2007
Last modified: February 19, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways