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A8H547

- RUVC_SHEPA

UniProt

A8H547 - RUVC_SHEPA

Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Shewanella pealeana (strain ATCC 700345 / ANG-SQ1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi8 – 81MagnesiumUniRule annotation
    Metal bindingi67 – 671MagnesiumUniRule annotation
    Metal bindingi139 – 1391MagnesiumUniRule annotation
    Metal bindingi142 – 1421MagnesiumUniRule annotation

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-HAMAP
    2. DNA repair Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciSPEA398579:GHG5-2487-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
    Alternative name(s):
    Holliday junction nuclease RuvCUniRule annotation
    Holliday junction resolvase RuvCUniRule annotation
    Gene namesi
    Name:ruvCUniRule annotation
    Ordered Locus Names:Spea_2364
    OrganismiShewanella pealeana (strain ATCC 700345 / ANG-SQ1)
    Taxonomic identifieri398579 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000002608: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 173173Crossover junction endodeoxyribonuclease RuvCPRO_1000074501Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi398579.Spea_2364.

    Structurei

    3D structure databases

    ProteinModelPortaliA8H547.
    SMRiA8H547. Positions 2-156.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuvC family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0817.
    HOGENOMiHOG000012181.
    KOiK01159.
    OMAiCIRTASD.
    OrthoDBiEOG6RG044.

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    HAMAPiMF_00034. RuvC.
    InterProiIPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view]
    PfamiPF02075. RuvC. 1 hit.
    [Graphical view]
    PRINTSiPR00696. RSOLVASERUVC.
    SUPFAMiSSF53098. SSF53098. 1 hit.
    TIGRFAMsiTIGR00228. ruvC. 1 hit.
    PROSITEiPS01321. RUVC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8H547-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAIILGVDPG SRITGYGVIK CVGRQQVYVG SGCIRTSSDE LPLRLKQIFD    50
    GLSEIIRQYQ PDEFAIERVF MAKNADSALK LGQARGAAIV AATAANLPVA 100
    EYSATQIKSA VVGTGRAQKT QVQHMIQQLL KLPSAPQADA ADALGVAVCH 150
    YHTSQSLVAL AGRANVRTYG RYK 173
    Length:173
    Mass (Da):18,458
    Last modified:November 13, 2007 - v1
    Checksum:i34DEDE4DE8B211DC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000851 Genomic DNA. Translation: ABV87684.1.
    RefSeqiYP_001502219.1. NC_009901.1.

    Genome annotation databases

    EnsemblBacteriaiABV87684; ABV87684; Spea_2364.
    GeneIDi5662757.
    KEGGispl:Spea_2364.
    PATRICi23534710. VBIShePea72822_2474.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000851 Genomic DNA. Translation: ABV87684.1 .
    RefSeqi YP_001502219.1. NC_009901.1.

    3D structure databases

    ProteinModelPortali A8H547.
    SMRi A8H547. Positions 2-156.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 398579.Spea_2364.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV87684 ; ABV87684 ; Spea_2364 .
    GeneIDi 5662757.
    KEGGi spl:Spea_2364.
    PATRICi 23534710. VBIShePea72822_2474.

    Phylogenomic databases

    eggNOGi COG0817.
    HOGENOMi HOG000012181.
    KOi K01159.
    OMAi CIRTASD.
    OrthoDBi EOG6RG044.

    Enzyme and pathway databases

    BioCyci SPEA398579:GHG5-2487-MONOMER.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    HAMAPi MF_00034. RuvC.
    InterProi IPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view ]
    Pfami PF02075. RuvC. 1 hit.
    [Graphical view ]
    PRINTSi PR00696. RSOLVASERUVC.
    SUPFAMi SSF53098. SSF53098. 1 hit.
    TIGRFAMsi TIGR00228. ruvC. 1 hit.
    PROSITEi PS01321. RUVC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700345 / ANG-SQ1.

    Entry informationi

    Entry nameiRUVC_SHEPA
    AccessioniPrimary (citable) accession number: A8H547
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3