Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A8H3I7 (BIOB_SHEPA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Spea_1801
OrganismShewanella pealeana (strain ATCC 700345 / ANG-SQ1) [Complete proteome] [HAMAP]
Taxonomic identifier398579 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 350350Biotin synthase HAMAP-Rule MF_01694
PRO_0000381622

Sites

Metal binding561Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding601Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1001Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1311Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1911Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2631Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A8H3I7 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: 9990C404A26AC659

FASTA35038,864
        10         20         30         40         50         60 
MPEVQLRNNW KREEIEALFA LPMNDLLFQA HSIHRQEFDP NEVQVSRLLS IKTGACPEDC 

        70         80         90        100        110        120 
KYCPQSARYD TGLEKERLLA METVLTEARS AKAAGASRFC MGAAWRNPKE RDMPYLKTMV 

       130        140        150        160        170        180 
EEVKALGMET CMTLGMLSAE QANTLADAGL DYYNHNLDTS PEYYGDVITT RTYQSRLDTL 

       190        200        210        220        230        240 
TNVRASGMKV CSGGIVGMGE KATDRAGLIQ QLANLEQHPD SVPINMLVKV EGTPFEKLDD 

       250        260        270        280        290        300 
LDPLEFVRTI AVARITMPKS RVRLSAGREN MSDELQAMCF FAGANSIFYG CKLLTTPNPE 

       310        320        330        340        350 
ENDDMSLFKR LGLHPEQGIA ATKEQDEAML AKAAAHQDKK SAAFYDAGAL 

« Hide

References

[1]"Complete sequence of Shewanella pealeana ATCC 700345."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., Zhao J.-S.Z., Manno D., Hawari J., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700345 / ANG-SQ1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000851 Genomic DNA. Translation: ABV87124.1.
RefSeqYP_001501659.1. NC_009901.1.

3D structure databases

ProteinModelPortalA8H3I7.
SMRA8H3I7. Positions 7-317.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING398579.Spea_1801.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV87124; ABV87124; Spea_1801.
GeneID5662196.
KEGGspl:Spea_1801.
PATRIC23533449. VBIShePea72822_1879.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2304484.

Enzyme and pathway databases

BioCycSPEA398579:GHG5-1890-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_SHEPA
AccessionPrimary (citable) accession number: A8H3I7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: November 13, 2007
Last modified: February 19, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways