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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Shewanella pealeana (strain ATCC 700345 / ANG-SQ1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68S-adenosyl-L-methionineUniRule annotation1
Binding sitei93S-adenosyl-L-methionineUniRule annotation1
Binding sitei120S-adenosyl-L-methionineUniRule annotation1
Active sitei143By similarity1
Binding sitei143S-adenosyl-L-methionineUniRule annotation1
Binding sitei147SubstrateUniRule annotation1
Binding sitei179SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Spea_1109
OrganismiShewanella pealeana (strain ATCC 700345 / ANG-SQ1)
Taxonomic identifieri398579 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000002608 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000844511 – 237tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST237

Interactioni

Protein-protein interaction databases

STRINGi398579.Spea_1109.

Structurei

3D structure databases

ProteinModelPortaliA8H1J9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni216 – 219Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073968.
KOiK03439.
OMAiIKLGHGV.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8H1J9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDVTTAEFN EEGKYLRKVR SFVLREGRLT KGQAQAMEQQ WPKIGLDYTP
60 70 80 90 100
EPIDLVEVFG READTVLEIG FGMGASLVAM AKAAPELNFI GIEVHKPGVG
110 120 130 140 150
ACLAEAAEAG VTNLRVYHHD AIEVLENSIA EGSLACVQLF FPDPWHKTRH
160 170 180 190 200
HKRRIVQAPF AELIRSKLKV GGVFHLATDW ENYSEHMLEV MTAAPGYKNQ
210 220 230
SATGDVVERP AHRPLTKFEA RGHRLGHGVW DLMFERV
Length:237
Mass (Da):26,457
Last modified:November 13, 2007 - v1
Checksum:iFCF1A1C112CFCAD6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000851 Genomic DNA. Translation: ABV86436.1.
RefSeqiWP_012154365.1. NC_009901.1.

Genome annotation databases

EnsemblBacteriaiABV86436; ABV86436; Spea_1109.
KEGGispl:Spea_1109.
PATRICi23531922. VBIShePea72822_1157.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000851 Genomic DNA. Translation: ABV86436.1.
RefSeqiWP_012154365.1. NC_009901.1.

3D structure databases

ProteinModelPortaliA8H1J9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi398579.Spea_1109.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV86436; ABV86436; Spea_1109.
KEGGispl:Spea_1109.
PATRICi23531922. VBIShePea72822_1157.

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073968.
KOiK03439.
OMAiIKLGHGV.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_SHEPA
AccessioniPrimary (citable) accession number: A8H1J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 13, 2007
Last modified: November 2, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.