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A8H1H3

- SYI_SHEPA

UniProt

A8H1H3 - SYI_SHEPA

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Shewanella pealeana (strain ATCC 700345 / ANG-SQ1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
Binding sitei608 – 6081ATPUniRule annotation
Metal bindingi903 – 9031ZincUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi923 – 9231ZincUniRule annotation
Metal bindingi926 – 9261ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSPEA398579:GHG5-1124-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Spea_1083
OrganismiShewanella pealeana (strain ATCC 700345 / ANG-SQ1)
Taxonomic identifieri398579 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000002608: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 940940Isoleucine--tRNA ligasePRO_1000088555Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi398579.Spea_1083.

Structurei

3D structure databases

ProteinModelPortaliA8H1H3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi605 – 6095"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKKRIELM.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8H1H3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKSTLNL PETEFPMRGN LANREPEMLK SWTENGLYQQ IRESRKDAKP
60 70 80 90 100
FILHDGPPYA NGDIHIGHSV NKILKDIIIK SKTMSGFDAP YIPGWDCHGL
110 120 130 140 150
PIELKVEQKV GKPGHKVTAA EFRQKCREYA AKQVDGQRDD FIRLGVFADW
160 170 180 190 200
QNPYLTMDYS TEANIVRSLS KVIDNGHLHK GVKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYEDKMSPAI DVAFTVVDKP ALLAKFGVNE YPHNISMVIW TTTPWTLPAN
260 270 280 290 300
RALAVGANVE YTLVEMVKEG QAQALVLATD LYESCVERFG AESHTVLGTV
310 320 330 340 350
AGSDLELLRF NHPFYDFDVP VILGDHVTVD SGTGVVHTAP GHGQDDFVVG
360 370 380 390 400
QKYGLEVANP VGDNGVYKPD TEIFAGLHVF KANKNVVELL EEKGALLNHV
410 420 430 440 450
QIKHSYPHCW RHKTPIIFRA TPQWFISMDQ KGLRQQALGE IEQTQWIPDW
460 470 480 490 500
GQSRIEKMVE NRPDWCISRQ RTWGVPITLF VHRETEELHP DSVSLMERVA
510 520 530 540 550
ARIEQEGIQA WWDLDASELL GDEADQYRKV TDTLDVWYDS GSTFSSVVAA
560 570 580 590 600
RPEFNGHPID LYLEGSDQHR GWFMSSLMIS TAMNGKAPYK QVLTHGFTVD
610 620 630 640 650
GQGRKMSKSV GNVIAPQHVT NKLGADILRL WVAATDYSGE MTVSDQILNR
660 670 680 690 700
SADAYRRIRN TARFLLANIN GFNPETDMVA VEDMVALDRW VVRRAAALQQ
710 720 730 740 750
ELLEAYDQYN FHLVTQKLMQ FCSVELGSFY LDIIKDRQYT AKEDSNARRS
760 770 780 790 800
CQSALYLISE AMVRWIAPIL SFTADEIWKL LPGERGEYVF TETWYQGLQS
810 820 830 840 850
VALESDLSDE YWDQLLAVRA EVNKVIENAR REKQVGGSLE AEVTLYADEA
860 870 880 890 900
LQAVLENLGD ELRFVLLTSK TEVVALAQAP SDAIETELAS LKLSLKKSEA
910 920 930 940
EKCERCWHHR EDVGAVAEHP TLCTRCVTNI EGEGETRQFA
Length:940
Mass (Da):105,917
Last modified:November 13, 2007 - v1
Checksum:iF02F559EEF10B1EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000851 Genomic DNA. Translation: ABV86410.1.
RefSeqiYP_001500945.1. NC_009901.1.

Genome annotation databases

EnsemblBacteriaiABV86410; ABV86410; Spea_1083.
GeneIDi5661482.
KEGGispl:Spea_1083.
PATRICi23531857. VBIShePea72822_1131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000851 Genomic DNA. Translation: ABV86410.1 .
RefSeqi YP_001500945.1. NC_009901.1.

3D structure databases

ProteinModelPortali A8H1H3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 398579.Spea_1083.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV86410 ; ABV86410 ; Spea_1083 .
GeneIDi 5661482.
KEGGi spl:Spea_1083.
PATRICi 23531857. VBIShePea72822_1131.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KKRIELM.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SPEA398579:GHG5-1124-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700345 / ANG-SQ1.

Entry informationi

Entry nameiSYI_SHEPA
AccessioniPrimary (citable) accession number: A8H1H3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 13, 2007
Last modified: November 26, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3