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A8H1H3

- SYI_SHEPA

UniProt

A8H1H3 - SYI_SHEPA

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Spea_1083
Organism
Shewanella pealeana (strain ATCC 700345 / ANG-SQ1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylate By similarity
Binding sitei608 – 6081ATP By similarity
Metal bindingi903 – 9031Zinc By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi923 – 9231Zinc By similarity
Metal bindingi926 – 9261Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSPEA398579:GHG5-1124-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Spea_1083
OrganismiShewanella pealeana (strain ATCC 700345 / ANG-SQ1)
Taxonomic identifieri398579 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000002608: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 940940Isoleucine--tRNA ligaseUniRule annotationPRO_1000088555Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi398579.Spea_1083.

Structurei

3D structure databases

ProteinModelPortaliA8H1H3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi605 – 6095"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKKRIELM.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8H1H3-1 [UniParc]FASTAAdd to Basket

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MSDYKSTLNL PETEFPMRGN LANREPEMLK SWTENGLYQQ IRESRKDAKP    50
FILHDGPPYA NGDIHIGHSV NKILKDIIIK SKTMSGFDAP YIPGWDCHGL 100
PIELKVEQKV GKPGHKVTAA EFRQKCREYA AKQVDGQRDD FIRLGVFADW 150
QNPYLTMDYS TEANIVRSLS KVIDNGHLHK GVKPVHWCTD CGSALAEAEV 200
EYEDKMSPAI DVAFTVVDKP ALLAKFGVNE YPHNISMVIW TTTPWTLPAN 250
RALAVGANVE YTLVEMVKEG QAQALVLATD LYESCVERFG AESHTVLGTV 300
AGSDLELLRF NHPFYDFDVP VILGDHVTVD SGTGVVHTAP GHGQDDFVVG 350
QKYGLEVANP VGDNGVYKPD TEIFAGLHVF KANKNVVELL EEKGALLNHV 400
QIKHSYPHCW RHKTPIIFRA TPQWFISMDQ KGLRQQALGE IEQTQWIPDW 450
GQSRIEKMVE NRPDWCISRQ RTWGVPITLF VHRETEELHP DSVSLMERVA 500
ARIEQEGIQA WWDLDASELL GDEADQYRKV TDTLDVWYDS GSTFSSVVAA 550
RPEFNGHPID LYLEGSDQHR GWFMSSLMIS TAMNGKAPYK QVLTHGFTVD 600
GQGRKMSKSV GNVIAPQHVT NKLGADILRL WVAATDYSGE MTVSDQILNR 650
SADAYRRIRN TARFLLANIN GFNPETDMVA VEDMVALDRW VVRRAAALQQ 700
ELLEAYDQYN FHLVTQKLMQ FCSVELGSFY LDIIKDRQYT AKEDSNARRS 750
CQSALYLISE AMVRWIAPIL SFTADEIWKL LPGERGEYVF TETWYQGLQS 800
VALESDLSDE YWDQLLAVRA EVNKVIENAR REKQVGGSLE AEVTLYADEA 850
LQAVLENLGD ELRFVLLTSK TEVVALAQAP SDAIETELAS LKLSLKKSEA 900
EKCERCWHHR EDVGAVAEHP TLCTRCVTNI EGEGETRQFA 940
Length:940
Mass (Da):105,917
Last modified:November 13, 2007 - v1
Checksum:iF02F559EEF10B1EB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000851 Genomic DNA. Translation: ABV86410.1.
RefSeqiWP_012154341.1. NC_009901.1.
YP_001500945.1. NC_009901.1.

Genome annotation databases

EnsemblBacteriaiABV86410; ABV86410; Spea_1083.
GeneIDi5661482.
KEGGispl:Spea_1083.
PATRICi23531857. VBIShePea72822_1131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000851 Genomic DNA. Translation: ABV86410.1 .
RefSeqi WP_012154341.1. NC_009901.1.
YP_001500945.1. NC_009901.1.

3D structure databases

ProteinModelPortali A8H1H3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 398579.Spea_1083.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV86410 ; ABV86410 ; Spea_1083 .
GeneIDi 5661482.
KEGGi spl:Spea_1083.
PATRICi 23531857. VBIShePea72822_1131.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KKRIELM.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SPEA398579:GHG5-1124-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700345 / ANG-SQ1.

Entry informationi

Entry nameiSYI_SHEPA
AccessioniPrimary (citable) accession number: A8H1H3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 13, 2007
Last modified: September 3, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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