A8H029 (TPMT_SHEPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiopurine S-methyltransferase
EC=2.1.1.67

Alternative name(s):
Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	Spea_0588

Organism

Shewanella pealeana (strain ATCC 700345 / ANG-SQ1) [Complete proteome] [HAMAP]

Taxonomic identifier

398579 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity HAMAP MF_00812.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_1000083764
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	45	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	66	1	S-adenosyl-L-methionine By similarity
	Binding site	123	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8H029 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 384F99E3F20C6DBF	FASTA	219	24,972
	10 20 30 40 50 60 MQPSFWHDKW DAQQVGFHLS AVNPLLVKYW PQLELDANAQ VFVPLCGKSL DMCFLAEQGH 70 80 90 100 110 120 EVLGCELNEL AVQQFYRENE LAFDISTLAE HQRFHTEQVT IYQGDIFSLD AAEMPNTQAF 130 140 150 160 170 180 YDRAALIAWP EEMRSAYARQ LAKLVPANST GLLITLDYPQ AELNGPPFAV SDDWVQANLA 190 200 210 TDFEIERLSS EDVLSENPRF VNKQVSWLTE SVYKLIRKG

References

[1]

"Complete sequence of Shewanella pealeana ATCC 700345." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Zhao J.-S.Z., Manno D., Hawari J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700345 / ANG-SQ1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000851 Genomic DNA. Translation: ABV85916.1.
RefSeq	YP_001500451.1. NC_009901.1.
3D structure databases
ProteinModelPortal	A8H029.
ModBase	Search...
Protein-protein interaction databases
STRING	A8H029.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5660988.
GenomeReviews	Gene locus Spea_0588 in contig CP000851_GR.
KEGG	spl:Spea_0588.
NMPDR	fig|398579.3.peg.517.
PATRIC	23530819. VBIShePea72822_0614.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0500.
HOGENOM	HBG444929.
OMA	FAVSNDW.
ProtClustDB	PRK13255.
Enzyme and pathway databases
BioCyc	SPEA398579:SPEA_0588-MONOMER.
Family and domain databases
HAMAP	MF_00812. Thiopur_methtran. [Tree]
InterPro	IPR022474. Thiopur_S-MeTfrase_Se/Te_detox. IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
KO	K00569.
PANTHER	PTHR10259. PTHR10259. 1 hit.
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
TIGRFAMs	TIGR03840. TMPT_Se_Te. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_SHEPA

Accession

Primary (citable) accession number: A8H029

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program