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A8GZ21 (ASSY_SHEPA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:Spea_0230
OrganismShewanella pealeana (strain ATCC 700345 / ANG-SQ1) [Complete proteome] [HAMAP]
Taxonomic identifier398579 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 412412Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_1000073831

Regions

Nucleotide binding20 – 289ATP By similarity

Sites

Binding site481ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site1001Citrulline By similarity
Binding site1051Citrulline By similarity
Binding site1301ATP; via amide nitrogen By similarity
Binding site1321Aspartate By similarity
Binding site1361Aspartate By similarity
Binding site1361Citrulline By similarity
Binding site1371Aspartate By similarity
Binding site1401Citrulline By similarity
Binding site1891Citrulline By similarity
Binding site1981Citrulline By similarity
Binding site2741Citrulline By similarity
Binding site2861Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
A8GZ21 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: D58FE5D0D8FC23D6

FASTA41244,979
        10         20         30         40         50         60 
MSIENSAVSD HSGVNKVVLA YSGGLDTSAI IPWLKETYDN CEIVAFCADV GQGDAELEGL 

        70         80         90        100        110        120 
YEKAIASGAS ECYIVDLKEE LVADYIYPTI ATGAIYEGTY LLGTSMARPI IAKAQVEVAR 

       130        140        150        160        170        180 
KVGADAVCHG CTGKGNDQVR FEGCFAALAP DLKVIAPWRE WEMVSREDLL DYLAERNIAT 

       190        200        210        220        230        240 
AASATKIYSR DANAWHISHE GGELEDPWNE PSKGVWTMTV APEDAPNQPE YVSLELEQGK 

       250        260        270        280        290        300 
ITKVNGEALS PYKALMVLNE VAGAHGVGRI DITENRLVGM KSRGCYETPG GTVMFAALRA 

       310        320        330        340        350        360 
IEELVLDKTS REWREQVGAQ MAHLVYDGRW FTPLCESLLG ASKPLADLVN GEVVVKLYKG 

       370        380        390        400        410 
QASVVKKRSP NSLYSEEFAT FGADDVYNQK DAEGFIRLYS LSSRIRALHS QK 

« Hide

References

[1]"Complete sequence of Shewanella pealeana ATCC 700345."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., Zhao J.-S.Z., Manno D., Hawari J., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700345 / ANG-SQ1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000851 Genomic DNA. Translation: ABV85558.1.
RefSeqYP_001500093.1. NC_009901.1.

3D structure databases

ProteinModelPortalA8GZ21.
SMRA8GZ21. Positions 16-410.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING398579.Spea_0230.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV85558; ABV85558; Spea_0230.
GeneID5660630.
KEGGspl:Spea_0230.
PATRIC23530036. VBIShePea72822_0238.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMAIYNGYWW.
OrthoDBEOG6K9QCV.
ProtClustDBPRK00509.

Enzyme and pathway databases

BioCycSPEA398579:GHG5-253-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_SHEPA
AccessionPrimary (citable) accession number: A8GZ21
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 13, 2007
Last modified: February 19, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways