A8GYH2 (FMT_SHEPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase
EC=2.1.2.9

Gene names

Name:	fmt
Ordered Locus Names:	Spea_0030

Organism

Shewanella pealeana (strain ATCC 700345 / ANG-SQ1) [Complete proteome] [HAMAP]

Taxonomic identifier

398579 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella › Shewanella pealeana

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182
Sequence similarities	Belongs to the fmt family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translational initiation Inferred from electronic annotation. Source: GOC
Molecular_function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: HAMAP methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182		PRO_1000077321
Regions
	Region	112 – 115	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8GYH2 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 4FF201030708FF06	FASTA	321	34,667
	10 20 30 40 50 60 MKPLNVIFAG TPDFAARHLQ ALIDSEHNVI AVYTQPDRPA GRGKKLQASP VKALALENDI 70 80 90 100 110 120 AVFQPKSLRD EDAQAELAAL NADIMVVVAY GLILPKVVLD TPRLGCINVH GSILPRWRGA 130 140 150 160 170 180 APIQRALWAG DTETGVTIMQ MDIGLDTGDM LLKTLLPIED NDTSSTLYEK LAEQGPTALV 190 200 210 220 230 240 EALAGIAEGT LPAEKQDESL ANYAEKLSKE EARLDWSKPA SALWREIRAF NPWPVSHYEH 250 260 270 280 290 300 EGNTIKVWQS SVSDETSSQQ PGTILSADKS GISIATGEGV LTITQMQLPG KKPLSVADIL 310 320 NSRADWFTPG TVLASTDNQE A

References

[1]

"Complete sequence of Shewanella pealeana ATCC 700345." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Zhao J.-S.Z., Manno D., Hawari J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700345 / ANG-SQ1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000851 Genomic DNA. Translation: ABV85359.1.
RefSeq	YP_001499894.1. NC_009901.1.
3D structure databases
ProteinModelPortal	A8GYH2.
SMR	A8GYH2. Positions 4-313.
ModBase	Search...
Protein-protein interaction databases
STRING	398579.Spea_0030.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV85359; ABV85359; Spea_0030.
GeneID	5660431.
KEGG	spl:Spea_0030.
PATRIC	23529574. VBIShePea72822_0030.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0223.
HOGENOM	HOG000261177.
KO	K00604.
OMA	GITLMQM.
ProtClustDB	CLSK905552.
Family and domain databases
Gene3D	3.10.25.10. 1 hit. 3.40.50.170. 1 hit.
HAMAP	MF_00182. Formyl_trans.
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR001555. GART_AS. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
PANTHER	PTHR11138. PTHR11138. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. fmt. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMT_SHEPA

Accession

Primary (citable) accession number: A8GYH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program