Reviewed, UniProtKB/Swiss-Prot A8GYG0 (FADB_SHEPA)

Names and origin

Protein names

Recommended name:
    Fatty acid oxidation complex subunit alpha
Including the following 2 domains:
    1- Recommended name:
            Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase
              EC=4.2.1.17
              EC=5.3.3.8
              EC=5.1.2.3
    2- Recommended name:
            3-hydroxyacyl-CoA dehydrogenase
              EC=1.1.1.35

Gene names

Name:	fadB
Ordered Locus Names:	Spea_0018

Organism

Shewanella pealeana (strain ATCC 700345 / ANG-SQ1) [Complete proteome] [HAMAP]

Taxonomic identifier

398579 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	717 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities By similarity. HAMAP MF_01621
Catalytic activity	(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH. HAMAP MF_01621 (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O. HAMAP MF_01621 (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA. HAMAP MF_01621 (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA. HAMAP MF_01621
Pathway	Lipid metabolism; fatty acid beta-oxidation. HAMAP MF_01621
Subunit structure	Heterotetramer of two alpha chains (fadB) and two beta chains (fadA) By similarity. HAMAP MF_01621
Sequence similarities	In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid degradation Lipid metabolism
Ligand	NAD
Molecular function	Isomerase Lyase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	fatty acid beta-oxidation multienzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	3-hydroxyacyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: HAMAP 3-hydroxybutyryl-CoA epimerase activity Inferred from electronic annotation. Source: HAMAP coenzyme binding Inferred from electronic annotation. Source: InterPro dodecenoyl-CoA delta-isomerase activity Inferred from electronic annotation. Source: HAMAP enoyl-CoA hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 717	717	Fatty acid oxidation complex subunit alpha HAMAP MF_01621		PRO_1000088084
Regions
	Nucleotide binding	400 – 402	3	NAD By similarity
	Nucleotide binding	427 – 429	3	NAD By similarity
	Region	1 – 189	189	Enoyl-CoA hydratase/isomerase By similarity
	Region	311 – 717	407	3-hydroxyacyl-CoA dehydrogenase By similarity
Sites
	Binding site	296	1	Substrate By similarity
	Binding site	324	1	NAD; via amide nitrogen By similarity
	Binding site	343	1	NAD By similarity
	Binding site	407	1	NAD By similarity
	Binding site	453	1	NAD By similarity
	Binding site	500	1	Substrate By similarity
	Binding site	660	1	Substrate By similarity
	Site	119	1	Important for catalytic activity By similarity
	Site	139	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8GYG0-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 6A5A3D3EB9EB3C68	FASTA	717	77,331
	10 20 30 40 50 60 MIYQSPTIEV ELLEDNIAHL CFKAQGSVNK FDRETIDSLN AALDSIKQDS SIKALMLSSA 70 80 90 100 110 120 KDAFVVGADI TEFLGLFAEE DTVLQSWLEQ ANVVFNKLED LPFPTISAIN GFALGAGCET 130 140 150 160 170 180 ILATDFRIAD TTARIGLPET KLGIIPGFGG TVRLPRVIGA DNALEWITSG KDQRPEAALK 190 200 210 220 230 240 VGAIDAVVAP EQLKPAALKM LKDALIEKLD WQTRRAKKQA PLTLPKLEAM MSFATAKGMV 250 260 270 280 290 300 FKVAGKHYPA PMAVISVIEQ AAQLDRAGAL QVEHQAFIKL AKTEVAQALI GIFLNDQLVK 310 320 330 340 350 360 GKAKKAGKLA KKVNSAAVLG AGIMGGGIAY QSASKGTPIV MKDIAQPALD LGLGEAAKLL 370 380 390 400 410 420 TAQVKRGRST PAKMAAVLNN ITPALDYAPV KDADVVVEAV VEHPKVKSMV LAEVEQHVSE 430 440 450 460 470 480 DAIITSNTST ISINLLAKSL KKPERFCGMH FFNPVHKMPL VEVIRGENSS DETVASVVAY 490 500 510 520 530 540 ASKMGKTPIV VNDCPGFFVN RVLFPYFAGF SGLLADGADF AAIDKVMEKQ FGWPMGPAYL 550 560 570 580 590 600 LDVVGLDTGH HAQAVMAEGF PDRMGKTGKD AIDVMFEAER FGQKNSKGFY QYSVDRRGKP 610 620 630 640 650 660 KKDLDPTSYE LLQAEFGEQK AFESDEIIAR TMIPMIIETV RCLEEGIIAS PAEADMGLVY 670 680 690 700 710 GLGFPPFRGG VFRYLDTMGV ANFVALADKY AHLGGLYQVT DTMRELAANN GSYYQQA

References

[1]

"Complete sequence of Shewanella pealeana ATCC 700345." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Zhao J.-S.Z., Manno D., Hawari J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000851 Genomic DNA. Translation: ABV85347.1.
RefSeq	YP_001499882.1.
3D structure databases
SMR	A8GYG0. Positions 1-714.
ModBase	Search...
Protein-protein interaction databases
STRING	A8GYG0.
Genome annotation databases
GeneID	5660419.
GenomeReviews	Gene locus Spea_0018 in contig CP000851_GR.
KEGG	spl:Spea_0018.
NMPDR	fig|398579.3.peg.18.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1250.
HOGENOM	HBG691737.
OMA	ASRFGQK.
Family and domain databases
HAMAP	MF_01621. FadB. [Tree]
InterPro	IPR006180. 3-OHacyl-CoA_DH_CS. IPR006176. 3-OHacyl-CoA_DH_NAD-bd. IPR006108. 3HC_DH_C. IPR008927. 6-PGluconate_DH_C-like. IPR001753. Crotonase_core. IPR013328. DH_multihelical. IPR018376. Enoyl-CoA_hyd/isom_CS. IPR012799. FadB. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF00725. 3HCDH. 1 hit. PF02737. 3HCDH_N. 1 hit. PF00378. ECH. 1 hit. [Graphical view]
TIGRFAMs	TIGR02437. FadB. 1 hit.
PROSITE	PS00067. 3HCDH. 1 hit. PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FADB_SHEPA

Accession

Primary (citable) accession number: A8GYG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 13, 2007
Last modified:	February 9, 2010
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)