Reviewed, UniProtKB/Swiss-Prot A8GYE1 (LEP_RICB8)

Names and origin

Protein names

Recommended name:
    Signal peptidase I
      Short name=SPase I
    EC=3.4.21.89
Alternative name(s):
    Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	A1I_01370

Organism

Rickettsia bellii (strain OSU 85-389) [Complete proteome] [HAMAP]

Taxonomic identifier

391896 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell inner membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Signal peptidase I		PRO_0000316272
Regions
	Topological domain	1 – 43	43	Cytoplasmic Potential
	Transmembrane	44 – 64	21	Potential
	Topological domain	65 – 289	225	Periplasmic Potential
Sites
	Active site	68	1	By similarity
	Active site	131	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8GYE1-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: F8A30C279F34650F	FASTA	289	33,889
	10 20 30 40 50 60 MKKLTSTTTT LWDNKLFINN LKNFMQTNTE SNNNKTTAQE WKSFILVVVI ALMIRILIIE 70 80 90 100 110 120 SFVVPTGSMK ATILENDRIF GTKYSYGYSN YSLSFFDFIH LFKGRIFART PERGDIIIFR 130 140 150 160 170 180 PPHEMNTRYI KRLIGLPGDK VQLIDDVIYI NDEKIERVES GIYVSEEGRK YLKFKETLPN 190 200 210 220 230 240 GKTYFSYKLA PVLGIMFNDK YGNTDAFYVP EGEYFFLGDN RDQSNDSRID LGFVPFENFI 250 260 270 280 AKAQFIWFST KITWWDSDIG VINLILKLKP WAESIRFNRI FRNLYSIED

References

[1]	"Complete genome sequencing of Rickettsia bellii." Madan A., Lee H., Madan A., Yoon J.-G., Ryu G.-Y., Dasch G., Ereemeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000849 Genomic DNA. Translation: ABV78666.1.
RefSeq	YP_001495703.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8GYE1.
Genome annotation databases
GeneID	5647291.
GenomeReviews	Gene locus A1I_01370 in contig CP000849_GR.
KEGG	rbo:A1I_01370.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KPWIESV.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_RICB8

Accession

Primary (citable) accession number: A8GYE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)