A8GY58 (SYS_RICB8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine--tRNA ligase
EC=6.1.1.11

Alternative name(s):
Seryl-tRNA synthetase
Short name=SerRS
Seryl-tRNA(Ser/Sec) synthetase

Gene names

Name:	serS
Ordered Locus Names:	A1I_07550

Organism

Rickettsia bellii (strain OSU 85-389) [Complete proteome] [HAMAP]

Taxonomic identifier

391896 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › belli group › Rickettsia bellii

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity. HAMAP-Rule MF_00176
Catalytic activity	ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). HAMAP-Rule MF_00176 ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec). HAMAP-Rule MF_00176
Pathway	Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. HAMAP-Rule MF_00176
Subunit structure	Homodimer. The tRNA molecule binds across the dimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity. HAMAP-Rule MF_00176
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	selenocysteine biosynthetic process Inferred from electronic annotation. Source: HAMAP selenocysteinyl-tRNA(Sec) biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway seryl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP serine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Serine--tRNA ligase HAMAP-Rule MF_00176		PRO_1000019799
Regions
	Nucleotide binding	260 – 262	3	ATP By similarity
	Nucleotide binding	347 – 350	4	ATP By similarity
	Region	229 – 231	3	Serine binding By similarity
Sites
	Binding site	283	1	Serine By similarity
	Binding site	383	1	Serine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8GY58 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 9564D3B0DF4149A5	FASTA	426	48,519
	10 20 30 40 50 60 MLNIKWIREN QELFDDKLRQ RFIEPMAKRI EELDGKKRKI TNLIQEFQHA RKVKSKILGN 70 80 90 100 110 120 INPKSGEEFE GLQRDVKDIN EKLEELEQDL NNNNELNELL NTLPNIPDEE VPYGIDESMN 130 140 150 160 170 180 KLIRTHGEVD LNAQNKKQHF ELGVKLDLMD FEQTAKISGA RFVTLKGDLA KLERALANFM 190 200 210 220 230 240 LDVHTGEFGF LEVSPPVLVR DNAMYNSGQL PKFADESFAT TNGYRLIPTA EVSLVNMVAD 250 260 270 280 290 300 TIIPREKLPM RLVAYTPCFR SEAGSSGRDT RGMIRLHQFS KVELVSITTP EESKNEHEYM 310 320 330 340 350 360 TNASETILQK LGLPYRTMLL CTGDMGFASQ KTYDIEVWLP GQKQYREIAS CSNCGDFQAR 370 380 390 400 410 420 RMKARYKEFG SHDTTLVHTL NASGLPIGRT MVAILENYQN EDGSITVPDV LVNYMGGLQK ITAYKE

References

[1]	"Complete genome sequencing of Rickettsia bellii." Madan A., Lee H., Madan A., Yoon J.-G., Ryu G.-Y., Dasch G., Ereemeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OSU 85-389.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000849 Genomic DNA. Translation: ABV79808.1.
RefSeq	YP_001496845.1. NC_009883.1.
3D structure databases
ProteinModelPortal	A8GY58.
ModBase	Search...
Protein-protein interaction databases
STRING	391896.A1I_07550.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV79808; ABV79808; A1I_07550.
GeneID	5646013.
KEGG	rbo:A1I_07550.
PATRIC	17881064. VBIRicBel35792_1578.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0172.
HOGENOM	HOG000035938.
KO	K01875.
OMA	EESWEWH.
ProtClustDB	PRK05431.
Enzyme and pathway databases
BioCyc	RBEL391896:GH75-1431-MONOMER.
UniPathway	UPA00906; UER00895.
Family and domain databases
Gene3D	1.10.287.40. 1 hit.
HAMAP	MF_00176. Ser_tRNA_synth_type1.
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR002317. Ser-tRNA-ligase_type_1. IPR015866. Ser-tRNA-synth_1_N. IPR010978. tRNA-bd_arm. [Graphical view]
PANTHER	PTHR11778. PTHR11778. 1 hit.
Pfam	PF02403. Seryl_tRNA_N. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTS	PR00981. TRNASYNTHSER.
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00414. serS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYS_RICB8

Accession

Primary (citable) accession number: A8GY58

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program