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Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Rickettsia bellii (strain OSU 85-389)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei243 – 2431ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRBEL391896:GH75-1018-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetase 2UniRule annotation
Short name:
GluRS 2UniRule annotation
Gene namesi
Name:gltX2UniRule annotation
Ordered Locus Names:A1I_05405
OrganismiRickettsia bellii (strain OSU 85-389)
Taxonomic identifieri391896 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group
ProteomesiUP000001152 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 464464Glutamate--tRNA ligase 2PRO_0000367755Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi391896.A1I_05405.

Structurei

3D structure databases

ProteinModelPortaliA8GX08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi11 – 2111"HIGH" regionAdd
BLAST
Motifi240 – 2445"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A8GX08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNNVITRFA PSPTGFLHIG SARTALFNYL FAKHNNGKFL LRIEDTDKER
60 70 80 90 100
STEAAVEAIF SGLKWLGLNW DDEVVFQSKR NDLYKEAALK LLAEGKAYYC
110 120 130 140 150
FTPQEEIEKQ RQEALENKQH FIFNSKWRDK TSDTYPKDIK PVIRLKTPSS
160 170 180 190 200
GSITIHDTLQ GDVVIENCHI DDMVLLRSDG TATYMLAVVV DDHDMGITHI
210 220 230 240 250
IRGDDHLTNA ARQIAIYNAF GYHVPIMTHI PLIHGADGAK LSKRHGALGV
260 270 280 290 300
EAYKDMGYLP ESLCNYLLRL GWSHGDDEII QMDQAIEWFN LDSLGKSPAR
310 320 330 340 350
LDFTKMNSLN SHYLRMLDED SLITKILEIL NRNYKVSEQE VNYIRRGLQG
360 370 380 390 400
LLVRSETLLD LAKLAKIYLV NIPVAYESEA KEIIANCDKN LINNVVQGLE
410 420 430 440 450
KLERFDKESV QDEFKKIAAA NSLKLNEVMK PVRALITGMV GSPSVFEIAE
460
ILGKENILKR LEIK
Length:464
Mass (Da):52,642
Last modified:March 23, 2009 - v2
Checksum:i3287F4E1269D22A0
GO

Sequence cautioni

The sequence ABV79408.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000849 Genomic DNA. Translation: ABV79408.1. Different initiation.
RefSeqiYP_001496445.1. NC_009883.1.

Genome annotation databases

EnsemblBacteriaiABV79408; ABV79408; A1I_05405.
KEGGirbo:A1I_05405.
PATRICi17880139. VBIRicBel35792_1126.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000849 Genomic DNA. Translation: ABV79408.1. Different initiation.
RefSeqiYP_001496445.1. NC_009883.1.

3D structure databases

ProteinModelPortaliA8GX08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391896.A1I_05405.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV79408; ABV79408; A1I_05405.
KEGGirbo:A1I_05405.
PATRICi17880139. VBIRicBel35792_1126.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciRBEL391896:GH75-1018-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequencing of Rickettsia bellii."
    Madan A., Lee H., Madan A., Yoon J.-G., Ryu G.-Y., Dasch G., Ereemeva M.
    Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OSU 85-389.

Entry informationi

Entry nameiSYE2_RICB8
AccessioniPrimary (citable) accession number: A8GX08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2009
Last sequence update: March 23, 2009
Last modified: March 31, 2015
This is version 47 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.