A8GWP2 (SYE1_RICB8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	A1I_04650

Organism

Rickettsia bellii (strain OSU 85-389) [Complete proteome] [HAMAP]

Taxonomic identifier

391896 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	Glutamate--tRNA ligase 1 HAMAP MF_00022_B		PRO_0000367754
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022_B
	Motif	240 – 244	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	243	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8GWP2 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 30EBB08D2FBF8905	FASTA	445	51,851
	10 20 30 40 50 60 MTKVITRFAP SPTGMLHVGN IRAALLNWLY AKKHDGQFIL RFDDTDLERS KQEYKDAIRA 70 80 90 100 110 120 DLKFLNLNWD QTFNQLSRLS RYDEIKKLLL DKKRLYACYE TPEELELKRK FQLSKGLPPI 130 140 150 160 170 180 YDRAALNLTE DQIQKYIEQG RKPHYRFLVN HEPITWHDMI KGEVKYEGKA LSDPIVIRAD 190 200 210 220 230 240 GSMTYMLCSV IDDVDYEITH IIRGEDHVSN TAIQIQMFEA LDKYPPTFGH LSLIINKDEK 250 260 270 280 290 300 ISKRVGGFEI ATLREEVGLE AMAIASFFSL LGSSAQIIPH KKMDELVKHF EISSFSKSPT 310 320 330 340 350 360 IYQPEDLERL NHKLLISLEF NEVKDRLKEI DAEYIDENFW LSVRPNLKKL FDAKDWWEIC 370 380 390 400 410 420 HKTPNIQDLN LDKEYLKQAA ELLPEEEITT ETWGIWTKKL AAITNRKGKE LFLPLRLALT 430 440 GKESGPEISK VLPLIKREEI VKRLT

References

[1]	"Complete genome sequencing of Rickettsia bellii." Madan A., Lee H., Madan A., Yoon J.-G., Ryu G.-Y., Dasch G., Ereemeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OSU 85-389.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000849 Genomic DNA. Translation: ABV79269.1.
RefSeq	YP_001496306.1. NC_009883.1.
3D structure databases
ProteinModelPortal	A8GWP2.
ModBase	Search...
Protein-protein interaction databases
STRING	A8GWP2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5646444.
GenomeReviews	Gene locus A1I_04650 in contig CP000849_GR.
KEGG	rbo:A1I_04650.
PATRIC	17879816. VBIRicBel35792_0967.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	LLYPCYE.
ProtClustDB	PRK12558.
Enzyme and pathway databases
BioCyc	RBEL391896:A1I_04650-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_RICB8

Accession

Primary (citable) accession number: A8GWP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program