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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Rickettsia bellii (strain OSU 85-389)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation.IEP:

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.IEP:

Cofactori

Mg2+HMP:, Mn2+HMP:Note: Binds 2 magnesium or manganese ions per subunit.HMP:

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.IEP:
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi268Magnesium or manganese 1IEP:1
Metal bindingi282Magnesium or manganese 1IEP:1
Metal bindingi282Magnesium or manganese 2IEP:1
Metal bindingi284Magnesium or manganese 2IEP:1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi148 – 199ATPIEP:Add BLAST52

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseIEP: (EC:6.3.2.4IEP:)
Alternative name(s):
D-Ala-D-Ala ligaseIEP:
D-alanylalanine synthetaseIEP:
Gene namesi
Name:ddlIEP:
Ordered Locus Names:A1I_03850
OrganismiRickettsia bellii (strain OSU 85-389)
Taxonomic identifieri391896 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000304871 – 321D-alanine--D-alanine ligaseAdd BLAST321

Structurei

3D structure databases

ProteinModelPortaliA8GW96.
SMRiA8GW96.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini121 – 315ATP-graspIEP:Add BLAST195

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.IEP:

Phylogenomic databases

eggNOGiENOG4105CPF. Bacteria.
COG1181. LUCA.
HOGENOMiHOG000011592.
KOiK01921.
OMAiYETKYTE.
OrthoDBiPOG091H00GT.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
HAMAPiMF_00047. Dala_Dala_lig. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom_sf.
PfamiView protein in Pfam
PF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
PIRSFiPIRSF039102. Ddl/VanB. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A8GW96-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHKYQTHWVE SSEIKILSDK GKKHIALVAG GMSAEREVSL ISAEGVGKAL
60 70 80 90 100
IEAGYKVTFI DMGADIAVKL HEIKPDIVFN CLHGTYGEDG CLPGLLNIMR
110 120 130 140 150
IPYTHSGVLA SSLAFDKVHS RSWFLTNNIN MAESIVISKG DNIKTDPIKR
160 170 180 190 200
PYVIKPFTQG SSIGVEVIFE EDDFNFANYD FPYGDEVIIE KYIKGRELQV
210 220 230 240 250
AILNGKALGA LEIKLLKNRF YDYETKYTEG FAEHLCPAPL PTDIYDKLLK
260 270 280 290 300
ESEKIYNTMN CKGAARAEFI LEDGTNKLYA LEINTHPGMT PLSIVPEIAA
310 320
YHGIDFVNLI EEILKTASFE S
Length:321
Mass (Da):35,791
Last modified:November 13, 2007 - v1
Checksum:i895D787E47700786
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000849 Genomic DNA. Translation: ABV79123.1.
RefSeqiWP_012151853.1. NC_009883.1.

Genome annotation databases

EnsemblBacteriaiABV79123; ABV79123; A1I_03850.
KEGGirbo:A1I_03850.

Similar proteinsi

Entry informationi

Entry nameiDDL_RICB8
AccessioniPrimary (citable) accession number: A8GW96
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 13, 2007
Last modified: November 22, 2017
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families