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A8GW18

- SYI_RICB8

UniProt

A8GW18 - SYI_RICB8

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Protein
Isoleucine--tRNA ligase
Gene
ileS, A1I_03430
Organism
Rickettsia bellii (strain OSU 85-389)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei657 – 6571ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciRBEL391896:GH75-644-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:A1I_03430
OrganismiRickettsia bellii (strain OSU 85-389)
Taxonomic identifieri391896 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group
ProteomesiUP000001152: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11081108Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022159Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi391896.A1I_03430.

Structurei

3D structure databases

ProteinModelPortaliA8GW18.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionUniRule annotation
Add
BLAST
Motifi654 – 6585"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiYETLYEC.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR022439. RPE4.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
TIGR03777. RPE4. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8GW18-1 [UniParc]FASTAAdd to Basket

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MTNTKYYPEV SSNADFATIE KEILKFWQDN NIFQKSIDIR EGDAEFIFYD     50
GPPFANGLPH YGHLLTGFIK DVYARYQTVR GKKVERRFGW DCHGLPAEMQ 100
SEKELGISGH LAITNFGIEK FNAHCRDSVM KYAGEWEQYV TRQARWVDFK 150
NSYKTMDKNF MESVLWAFKE LYNKGLLYES MRVMPYSWAC ETPLSNFETR 200
LDNSYRERAD KAVTVSFVLC HPVSKSTLDV IPWLDHGIQK TINNDSANCS 250
MDPVDKPRYD TENFLGITAN YKEYRILAWT TTTWTLPSNL ALAVGSDIDY 300
ALVPKGDVCY IIAASSVSKY AKELELKGDE QFTIIKGSEL QGLSYKPLFD 350
YFKNHPNSFK IFAGDFVVEG DGTGVVHMAP GFGEDDQILC ESKGIKLVCP 400
VDNSGKFTKE IPDLEGLQVF DANDKIIIKL KEQGNWLKTE QYIHNYPHCW 450
RTDTPLIYKA VPSWYVKVTE FKDRMVELNQ QINWIPTHVK DNLFGKWLEN 500
ARDWSISRNR FWGTPLPVWK SDDPKYPRID VYGSIEELEK DFGVKITDLH 550
RPFIDELTRA NPDDPTGKST MRRIEDVFDC WFESGSMPYG QAHYPFENKQ 600
WFEEHFPADF IVEYSAQTRG WFYTLMVLST ALFDRPPFLN CICHGVILDA 650
TGQKLSKRLN NYADPLELFD KYGSDALRVT MLSSNVVKGQ ELLIDKDGKM 700
VFDTLRLFIK PIWNAYHFFT MYANADHIKG ELNFTSENVL DVYILSKLKI 750
AVEKIKESLD SFDTGTAYHA VSEFFEVLNN WYIRRSRARF WKSEKDADKQ 800
SAYNTLYTCL ETMAIAMSSL VPLISEAIYL GLYCHPRKSG DPEKPECLDS 850
RLCGNDNLSV HLCDYPDLSK FKINSELVDT MDTVLDICNH SLFIRSSENA 900
RVRQPLSSIT IISKNNDKLK SFEDLIKDEI NVKSVIYRDD LENYAVKKLS 950
INFPLLGKRL PAKMKDIIAA SKKNEWEVTS GSLIICNETL NSDEYKLILE 1000
PHSHIKGASS FAHNSSLLIL DLELTPELID EGIARDIVRF IQQARKNADF 1050
AITDRILIDI NLPKITDIYG EFIKEQTLGK FAKDFIPDHI SEIELDNHKL 1100
QLKIKKVN 1108
Length:1,108
Mass (Da):127,564
Last modified:November 13, 2007 - v1
Checksum:i641A2AE9DC46BAB4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000849 Genomic DNA. Translation: ABV79045.1.
RefSeqiYP_001496082.1. NC_009883.1.

Genome annotation databases

EnsemblBacteriaiABV79045; ABV79045; A1I_03430.
GeneIDi5646403.
KEGGirbo:A1I_03430.
PATRICi17879292. VBIRicBel35792_0716.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000849 Genomic DNA. Translation: ABV79045.1 .
RefSeqi YP_001496082.1. NC_009883.1.

3D structure databases

ProteinModelPortali A8GW18.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 391896.A1I_03430.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV79045 ; ABV79045 ; A1I_03430 .
GeneIDi 5646403.
KEGGi rbo:A1I_03430.
PATRICi 17879292. VBIRicBel35792_0716.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi YETLYEC.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci RBEL391896:GH75-644-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR022439. RPE4.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
TIGR03777. RPE4. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequencing of Rickettsia bellii."
    Madan A., Lee H., Madan A., Yoon J.-G., Ryu G.-Y., Dasch G., Ereemeva M.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OSU 85-389.

Entry informationi

Entry nameiSYI_RICB8
AccessioniPrimary (citable) accession number: A8GW18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 13, 2007
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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