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A8GW18

- SYI_RICB8

UniProt

A8GW18 - SYI_RICB8

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rickettsia bellii (strain OSU 85-389)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei657 – 6571ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciRBEL391896:GH75-644-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:A1I_03430
    OrganismiRickettsia bellii (strain OSU 85-389)
    Taxonomic identifieri391896 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group
    ProteomesiUP000001152: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11081108Isoleucine--tRNA ligasePRO_1000022159Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi391896.A1I_03430.

    Structurei

    3D structure databases

    ProteinModelPortaliA8GW18.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi53 – 6311"HIGH" regionAdd
    BLAST
    Motifi654 – 6585"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiYETLYEC.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR022439. RPE4.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    TIGR03777. RPE4. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8GW18-1 [UniParc]FASTAAdd to Basket

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    MTNTKYYPEV SSNADFATIE KEILKFWQDN NIFQKSIDIR EGDAEFIFYD     50
    GPPFANGLPH YGHLLTGFIK DVYARYQTVR GKKVERRFGW DCHGLPAEMQ 100
    SEKELGISGH LAITNFGIEK FNAHCRDSVM KYAGEWEQYV TRQARWVDFK 150
    NSYKTMDKNF MESVLWAFKE LYNKGLLYES MRVMPYSWAC ETPLSNFETR 200
    LDNSYRERAD KAVTVSFVLC HPVSKSTLDV IPWLDHGIQK TINNDSANCS 250
    MDPVDKPRYD TENFLGITAN YKEYRILAWT TTTWTLPSNL ALAVGSDIDY 300
    ALVPKGDVCY IIAASSVSKY AKELELKGDE QFTIIKGSEL QGLSYKPLFD 350
    YFKNHPNSFK IFAGDFVVEG DGTGVVHMAP GFGEDDQILC ESKGIKLVCP 400
    VDNSGKFTKE IPDLEGLQVF DANDKIIIKL KEQGNWLKTE QYIHNYPHCW 450
    RTDTPLIYKA VPSWYVKVTE FKDRMVELNQ QINWIPTHVK DNLFGKWLEN 500
    ARDWSISRNR FWGTPLPVWK SDDPKYPRID VYGSIEELEK DFGVKITDLH 550
    RPFIDELTRA NPDDPTGKST MRRIEDVFDC WFESGSMPYG QAHYPFENKQ 600
    WFEEHFPADF IVEYSAQTRG WFYTLMVLST ALFDRPPFLN CICHGVILDA 650
    TGQKLSKRLN NYADPLELFD KYGSDALRVT MLSSNVVKGQ ELLIDKDGKM 700
    VFDTLRLFIK PIWNAYHFFT MYANADHIKG ELNFTSENVL DVYILSKLKI 750
    AVEKIKESLD SFDTGTAYHA VSEFFEVLNN WYIRRSRARF WKSEKDADKQ 800
    SAYNTLYTCL ETMAIAMSSL VPLISEAIYL GLYCHPRKSG DPEKPECLDS 850
    RLCGNDNLSV HLCDYPDLSK FKINSELVDT MDTVLDICNH SLFIRSSENA 900
    RVRQPLSSIT IISKNNDKLK SFEDLIKDEI NVKSVIYRDD LENYAVKKLS 950
    INFPLLGKRL PAKMKDIIAA SKKNEWEVTS GSLIICNETL NSDEYKLILE 1000
    PHSHIKGASS FAHNSSLLIL DLELTPELID EGIARDIVRF IQQARKNADF 1050
    AITDRILIDI NLPKITDIYG EFIKEQTLGK FAKDFIPDHI SEIELDNHKL 1100
    QLKIKKVN 1108
    Length:1,108
    Mass (Da):127,564
    Last modified:November 13, 2007 - v1
    Checksum:i641A2AE9DC46BAB4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000849 Genomic DNA. Translation: ABV79045.1.
    RefSeqiYP_001496082.1. NC_009883.1.

    Genome annotation databases

    EnsemblBacteriaiABV79045; ABV79045; A1I_03430.
    GeneIDi5646403.
    KEGGirbo:A1I_03430.
    PATRICi17879292. VBIRicBel35792_0716.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000849 Genomic DNA. Translation: ABV79045.1 .
    RefSeqi YP_001496082.1. NC_009883.1.

    3D structure databases

    ProteinModelPortali A8GW18.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 391896.A1I_03430.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV79045 ; ABV79045 ; A1I_03430 .
    GeneIDi 5646403.
    KEGGi rbo:A1I_03430.
    PATRICi 17879292. VBIRicBel35792_0716.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi YETLYEC.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci RBEL391896:GH75-644-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR022439. RPE4.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    TIGR03777. RPE4. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequencing of Rickettsia bellii."
      Madan A., Lee H., Madan A., Yoon J.-G., Ryu G.-Y., Dasch G., Ereemeva M.
      Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: OSU 85-389.

    Entry informationi

    Entry nameiSYI_RICB8
    AccessioniPrimary (citable) accession number: A8GW18
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3