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A8GW18

- SYI_RICB8

UniProt

A8GW18 - SYI_RICB8

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rickettsia bellii (strain OSU 85-389)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei657 – 6571ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciRBEL391896:GH75-644-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:A1I_03430
OrganismiRickettsia bellii (strain OSU 85-389)
Taxonomic identifieri391896 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group
ProteomesiUP000001152: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11081108Isoleucine--tRNA ligasePRO_1000022159Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi391896.A1I_03430.

Structurei

3D structure databases

ProteinModelPortaliA8GW18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionAdd
BLAST
Motifi654 – 6585"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiYETLYEC.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR022439. RPE4.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
TIGR03777. RPE4. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8GW18-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTNTKYYPEV SSNADFATIE KEILKFWQDN NIFQKSIDIR EGDAEFIFYD
60 70 80 90 100
GPPFANGLPH YGHLLTGFIK DVYARYQTVR GKKVERRFGW DCHGLPAEMQ
110 120 130 140 150
SEKELGISGH LAITNFGIEK FNAHCRDSVM KYAGEWEQYV TRQARWVDFK
160 170 180 190 200
NSYKTMDKNF MESVLWAFKE LYNKGLLYES MRVMPYSWAC ETPLSNFETR
210 220 230 240 250
LDNSYRERAD KAVTVSFVLC HPVSKSTLDV IPWLDHGIQK TINNDSANCS
260 270 280 290 300
MDPVDKPRYD TENFLGITAN YKEYRILAWT TTTWTLPSNL ALAVGSDIDY
310 320 330 340 350
ALVPKGDVCY IIAASSVSKY AKELELKGDE QFTIIKGSEL QGLSYKPLFD
360 370 380 390 400
YFKNHPNSFK IFAGDFVVEG DGTGVVHMAP GFGEDDQILC ESKGIKLVCP
410 420 430 440 450
VDNSGKFTKE IPDLEGLQVF DANDKIIIKL KEQGNWLKTE QYIHNYPHCW
460 470 480 490 500
RTDTPLIYKA VPSWYVKVTE FKDRMVELNQ QINWIPTHVK DNLFGKWLEN
510 520 530 540 550
ARDWSISRNR FWGTPLPVWK SDDPKYPRID VYGSIEELEK DFGVKITDLH
560 570 580 590 600
RPFIDELTRA NPDDPTGKST MRRIEDVFDC WFESGSMPYG QAHYPFENKQ
610 620 630 640 650
WFEEHFPADF IVEYSAQTRG WFYTLMVLST ALFDRPPFLN CICHGVILDA
660 670 680 690 700
TGQKLSKRLN NYADPLELFD KYGSDALRVT MLSSNVVKGQ ELLIDKDGKM
710 720 730 740 750
VFDTLRLFIK PIWNAYHFFT MYANADHIKG ELNFTSENVL DVYILSKLKI
760 770 780 790 800
AVEKIKESLD SFDTGTAYHA VSEFFEVLNN WYIRRSRARF WKSEKDADKQ
810 820 830 840 850
SAYNTLYTCL ETMAIAMSSL VPLISEAIYL GLYCHPRKSG DPEKPECLDS
860 870 880 890 900
RLCGNDNLSV HLCDYPDLSK FKINSELVDT MDTVLDICNH SLFIRSSENA
910 920 930 940 950
RVRQPLSSIT IISKNNDKLK SFEDLIKDEI NVKSVIYRDD LENYAVKKLS
960 970 980 990 1000
INFPLLGKRL PAKMKDIIAA SKKNEWEVTS GSLIICNETL NSDEYKLILE
1010 1020 1030 1040 1050
PHSHIKGASS FAHNSSLLIL DLELTPELID EGIARDIVRF IQQARKNADF
1060 1070 1080 1090 1100
AITDRILIDI NLPKITDIYG EFIKEQTLGK FAKDFIPDHI SEIELDNHKL

QLKIKKVN
Length:1,108
Mass (Da):127,564
Last modified:November 13, 2007 - v1
Checksum:i641A2AE9DC46BAB4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000849 Genomic DNA. Translation: ABV79045.1.
RefSeqiYP_001496082.1. NC_009883.1.

Genome annotation databases

EnsemblBacteriaiABV79045; ABV79045; A1I_03430.
GeneIDi5646403.
KEGGirbo:A1I_03430.
PATRICi17879292. VBIRicBel35792_0716.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000849 Genomic DNA. Translation: ABV79045.1 .
RefSeqi YP_001496082.1. NC_009883.1.

3D structure databases

ProteinModelPortali A8GW18.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 391896.A1I_03430.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV79045 ; ABV79045 ; A1I_03430 .
GeneIDi 5646403.
KEGGi rbo:A1I_03430.
PATRICi 17879292. VBIRicBel35792_0716.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi YETLYEC.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci RBEL391896:GH75-644-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR022439. RPE4.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
TIGR03777. RPE4. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequencing of Rickettsia bellii."
    Madan A., Lee H., Madan A., Yoon J.-G., Ryu G.-Y., Dasch G., Ereemeva M.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OSU 85-389.

Entry informationi

Entry nameiSYI_RICB8
AccessioniPrimary (citable) accession number: A8GW18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 13, 2007
Last modified: November 26, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3